Smolke:Protocols/Pfu Buffer: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
m (Smolke:Pfu Buffer moved to Smolke:Protocols/Pfu Buffer) |
||
(2 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
{{Smolke_Top}} | |||
==Summary== | ==Summary== | ||
Line 18: | Line 20: | ||
==Notes== | ==Notes== | ||
*We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -[[User:Jkm|Jkm]] 18:18, 2 August 2006 (EDT) | *We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -[[User:Jkm|Jkm]] 18:18, 2 August 2006 (EDT) | ||
*Should be supplemented with MgCl<sub>2</sub> | *Should be supplemented with MgCl<sub>2</sub> when used. A working concentration of 2.5mM (a 1/20 dilution of 50mM stock) works for most applications. | ||
==Source== | ==Source== |
Latest revision as of 14:08, 20 October 2009
Summary10x buffer for PCR with Pfu. Makes 10mL. Ingredients
Protocol
Notes
SourceUnknown. Ask Kristy. Contact |