Smolke:Protocols/Pfu Buffer

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Contents

Summary

10x buffer for PCR with Pfu. Makes 10mL.

Ingredients

  • 2 mL 1M Tris-HCl pH 8.8
  • 333 μL 3M KCl
  • 500 μL 20% Triton X-100
  • 1 mL 100x BSA
  • 200 μL 1M MgSO4
  • 5.967 mL H2O

Protocol

  1. Mix, vortex.
  2. Store at -20°C

Notes

  • We couldn't find any Triton X-100 in the lab. Our best guess is that NP-40 was (previously) used instead. -Jkm 18:18, 2 August 2006 (EDT)
  • Should be supplemented with MgCl2 when used. A working concentration of 2.5mM (a 1/20 dilution of 50mM stock) works for most applications.

Source

Unknown. Ask Kristy.

Contact

Kristy

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