Smolke:Protocols/Plasmid prep: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: *Follow Qiagen's QiaPrep Miniprep protocol, but skip step 7 (the Buffer PB wash). *Aliquot buffers from main stock prepared by Andy. **When you aliquot from the stocks please use careful, ...) |
m (Smolke:Plasmid prep moved to Smolke:Protocols/Plasmid prep) |
(No difference)
|
Revision as of 11:31, 15 October 2009
- Follow Qiagen's QiaPrep Miniprep protocol, but skip step 7 (the Buffer PB wash).
- Aliquot buffers from main stock prepared by Andy.
- When you aliquot from the stocks please use careful, sterile technique (even if you don't use sterile technique on your own stocks).
- Take aliquots of ~15 ml of P1, P2, N3 in Falcon tubes and keep on your bench.
- PE is made as a 5x stock (specifically, ethanol is not added). So, take an aliquot and add 4 volumes of EtOH to it (i.e., take 3 ml of stock and add 15 ml of EtOH). Make this up in 10-15 ml (final 1x volume) and make a new stock each month.
- Make aliquots of EB in 1-2 ml.