Smolke:Protocols/Plasmid prep

Home        Contact        Internal        Protocols        Lab Members        Publications        Research       

Follow Qiagen's QiaPrep Miniprep protocol, but skip step 7 (the Buffer PB wash). Aliquot buffers from main stock prepared by Andy. When you aliquot from the stocks please use careful, sterile technique (even if you don't use sterile technique on your own stocks). Take aliquots of ~15 ml of P1, P2, N3 in Falcon tubes and keep on your bench. PE is made as a 5x stock (specifically, ethanol is not added). So, take an aliquot and add 4 volumes of EtOH to it (i.e., take 3 ml of stock and add 15 ml of EtOH). Make this up in 10-15 ml (final 1x volume) and make a new stock each month. Make aliquots of EB in 1-2 ml.