Smolke:Protocols/Screening: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
(→Notes) |
||
Line 42: | Line 42: | ||
===Notes=== | ===Notes=== | ||
*Ideally, use primer sets that would generate a band for both positive and negative colonies but with different sizes. | *Ideally, use primer sets that would generate a band for both positive and negative colonies but with different sizes. | ||
*Should always include a negative control using the | *Should always include a negative control using the parent vector, no DNA template, or another appropriate choice. This would let you know if you have primer dimer or other nonspecific amplification. | ||
*Should include a positive control if possible. This is especially important if your chosen primers would only generate a band for positive but not negative colonies. | *Should include a positive control if possible. This is especially important if your chosen primers would only generate a band for positive but not negative colonies. |
Revision as of 14:41, 15 October 2009
Josh's ProtocolSample Preparation
Reaction Mixture
Yvonne's ProtocolProcedure
Notes
|