Smolke:Protocols/dNTP Mixture: Difference between revisions
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*100 mM TTP | *100 mM TTP | ||
*dH<sub>2</sub>O, preferably DEPC treated | *dH<sub>2</sub>O, preferably DEPC treated | ||
==Protocol== | |||
#Add 125 μL each NTP. | |||
#Add 750 μL dH<sub>2</sub>O. | |||
#Mix, Vortex. | |||
#Aliquot out 50 μL of mixture into 0.6 mL microfuge tubes. | |||
Revision as of 12:12, 21 July 2009
Summary
dNTP mixture: 40 mM NTPs, 10 mM each. Makes 1.25mL for a total of 25, 50 μL aliquots.
Ingredients
- 100 mM ATP
- 100 mM CTP
- 100 mM GTP
- 100 mM TTP
- dH2O, preferably DEPC treated
Protocol
- Add 125 μL each NTP.
- Add 750 μL dH2O.
- Mix, Vortex.
- Aliquot out 50 μL of mixture into 0.6 mL microfuge tubes.
