Smolke:Protocols/dNTP Mixture

From OpenWetWare

< Smolke:Protocols
Revision as of 16:09, 20 October 2009 by Josh K. Michener (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Home        Contact        Internal        Protocols        Lab Members        Publications        Research       


Contents

Summary

dNTP mixture: 40 mM NTPs, 10 mM each. Makes 1.25mL for a total of 25, 50 μL aliquots.

Ingredients

  • 100 mM ATP
  • 100 mM CTP
  • 100 mM GTP
  • 100 mM TTP
  • dH2O, preferably DEPC treated

Protocol

  1. Add 125 μL each NTP.
  2. Add 750 μL dH2O.
  3. Mix, Vortex.
  4. Aliquot out 50 μL of mixture into 0.6 mL microfuge tubes.

Source

Final concentrations based on NEB dNTP pre-mixed solution. Additional questions, ask Midori.

Personal tools