Sobeck Lab

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==Lab News==
 
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More information on its way...
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===WELCOME TO THE SOBECK LAB===
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We are interested in signaling pathways that contribute to the maintenance of genomic stability in our cells and prevent cancer susceptibility in humans. We are studying DNA repair pathways
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that are activated during the S-phase of the cell cycle when chromosomes replicate.
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Summer is almost here :) [[image:Frog10.gif]]
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Our research centers on the  Fanconi Anemia (FA) and Breast Cancer associated (BRCA1, BRCA2) repair pathways that are suspected have crucial functions in DNA repair and cell cycle checkpoint activation during S-phase. Humans born with mutations in FA genes have a highly elevated risk to develop certain types of cancers including acute myeloid leukemia (AML) and various types of solid tumors. Acquired mutations and silencing of FA genes have been found in different cancers in the general population and the FA genes are thus considered "caretakers" of our genome. Our goal is to understand the molecular functions of the FA/BRCA proteins (currently 15 FA/BRCA genes are known) and to elucidate how these proteins communicate with other members of the DNA damage response network to control and suppress genomic instability.
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==Research Interest==
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Our lab uses a combination of human cell-based assays and extracts prepared from eggs of the African clawed frog,'' Xenopus laevis''. These extracts are naturally cell-cycle synchronized and able to replicate and repair DNA in a test tube, providing us with a cell-free system that can be manipulated in many ways and that allows us to ask specific questions about functions of individual FA/BRCA proteins during the DNA damage response.
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We and others have shown that the upstream FA core complex is required for replication-dependent activation (monoubiquitination) of the downstream FANCD2 protein in response to DNA damaging agents that stall the replication fork. Our lab could also demonstrate that depletion of FA proteins from Xenopus laevis egg extracts results in accumulation of chromosomal breaks during replicative DNA synthesis.
 
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To further elucidate FA pathway function(s) in the replication-coupled DNA damage response I am interested in determining which DNA repair intermediates lead to specific recruitment and activation of the FA proteins. I am currently developing an activation assay in Xenopus egg extracts to test DNA minisubstrates for their ability to recruit and activate the FA core complex and/or FANCD2. Within the same experimental setting, I am using single-protein depletion strategies to investigate how the FA pathway is intertwined with other central DNA repair pathways in the DNA structure-specific damage response.
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[[Image:Sobeck GROUPsmaller.jpg]]
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'''May 2013 (left to right): Maya Raghunandan, Dan Stroik, Alex Sobeck, Jung-Eun Yeo, Indrajit Chaudhury, Sarah Riman, Niki Sathish Kumar'''
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===Lab News===
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<span style="color:blue">!! Mark your calendars for the Fanconi Anemia Research Fund Symposium from October 24-27, 2013 in Houston, Texas</span>
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  [[image:Frog10.gif]]
==Useful links==
==Useful links==
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]
*[[Help|OpenWetWare help pages]]

Revision as of 14:44, 23 May 2013

Home/Research        Contact        Lab Members        Publications        Talks        Protocols        Internal       



WELCOME TO THE SOBECK LAB

We are interested in signaling pathways that contribute to the maintenance of genomic stability in our cells and prevent cancer susceptibility in humans. We are studying DNA repair pathways that are activated during the S-phase of the cell cycle when chromosomes replicate.

Our research centers on the Fanconi Anemia (FA) and Breast Cancer associated (BRCA1, BRCA2) repair pathways that are suspected have crucial functions in DNA repair and cell cycle checkpoint activation during S-phase. Humans born with mutations in FA genes have a highly elevated risk to develop certain types of cancers including acute myeloid leukemia (AML) and various types of solid tumors. Acquired mutations and silencing of FA genes have been found in different cancers in the general population and the FA genes are thus considered "caretakers" of our genome. Our goal is to understand the molecular functions of the FA/BRCA proteins (currently 15 FA/BRCA genes are known) and to elucidate how these proteins communicate with other members of the DNA damage response network to control and suppress genomic instability.

Our lab uses a combination of human cell-based assays and extracts prepared from eggs of the African clawed frog, Xenopus laevis. These extracts are naturally cell-cycle synchronized and able to replicate and repair DNA in a test tube, providing us with a cell-free system that can be manipulated in many ways and that allows us to ask specific questions about functions of individual FA/BRCA proteins during the DNA damage response.




Image:Sobeck GROUPsmaller.jpg



May 2013 (left to right): Maya Raghunandan, Dan Stroik, Alex Sobeck, Jung-Eun Yeo, Indrajit Chaudhury, Sarah Riman, Niki Sathish Kumar

Lab News


!! Mark your calendars for the Fanconi Anemia Research Fund Symposium from October 24-27, 2013 in Houston, Texas

 image:Frog10.gif

Useful links

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