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lysis buffer [[Lysozyme]]
Revision as of 01:11, 29 April 2005
- Prepare lysis buffer by adding 10mg/ml Lysozyme to Pierce B-per Reagent.
- Spin down an overnight culture of cells at 2000rpm for 2 minutes to bring down large clumps of debris. The cells in the supernatant (which should still be cloudy) will serve as the cells to innoculate the device.