SortoStat/Operation: Difference between revisions
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lysis buffer | =Steps= | ||
Pierce B-per Reagent | |||
#Prepare lysis buffer by adding 10mg/ml [[Lysozyme]] to Pierce B-per Reagent. | |||
#Spin down an overnight culture of cells at 2000rpm for 2 minutes to bring down large clumps of debris. The cells in the supernatant (which should still be cloudy) will serve as the cells to innoculate the device. | |||
Revision as of 22:11, 28 April 2005
Steps
- Prepare lysis buffer by adding 10mg/ml Lysozyme to Pierce B-per Reagent.
- Spin down an overnight culture of cells at 2000rpm for 2 minutes to bring down large clumps of debris. The cells in the supernatant (which should still be cloudy) will serve as the cells to innoculate the device.
