Sortostat - Revision history

Dr. Richard Gordon at 01:53, 5 May 2008

Dr. Richard Gordon — Mon, 05 May 2008 01:53:26 GMT

←Older revision		Revision as of 01:53, 5 May 2008
Line 11:		Line 11:

	The design of the Sortostat is an extension of the microchemostat design created by Frederick Balagadde. ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15994559&query_hl=1&itool=pubmed_docsum REF])		The design of the Sortostat is an extension of the microchemostat design created by Frederick Balagadde. ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15994559&query_hl=1&itool=pubmed_docsum REF])
		+
		+	A proposal for a somewhat similar device is:
		+
		+	Gordon, R. 1996. Computer controlled evolution of diatoms: design for a compustat. Nova Hedwigia 112: 213-216.

	==Protocols==		==Protocols==

Jason R. Kelly — Thu, 07 Jun 2007 23:45:33 GMT

Experimental Results

←Older revision		Revision as of 23:45, 7 June 2007
Line 36:		Line 36:
	*[[Sortostat/Experiments/Brian growth tests]]		*[[Sortostat/Experiments/Brian growth tests]]
	*[[Sortostat/Experiments/Two reactor chip]]		*[[Sortostat/Experiments/Two reactor chip]]
		+	*[[Sortostat/Experiments/Comparison of CFP and YFP cells growth rate]]

	==Posters==		==Posters==

Jason R. Kelly — Thu, 07 Jun 2007 23:45:00 GMT

Known Issues

←Older revision		Revision as of 23:45, 7 June 2007
Line 30:		Line 30:
	#Control lines		#Control lines
	#*Over time the control lines empty of water. This was observed with green food dye.		#*Over time the control lines empty of water. This was observed with green food dye.
		+	#*This problem can be addressed with teflon tubing says the foundry.

	==Experimental Results==		==Experimental Results==

Jason R. Kelly — Tue, 29 May 2007 21:22:37 GMT

Known Issues: seems to be solved with the new chip design (less control lines)

←Older revision		Revision as of 21:22, 29 May 2007
Line 28:		Line 28:
	#*This can happen as a result of closing both the ClockWise and CounterClockWise valves, resulting in a pressure build up in the chambers between push-UP valves. It is not clear how pervasive a problem this is, but I have made changes to the [[Microfluidic chemostat labview program]] to close the CW and CCW sequentially to help the problem.		#*This can happen as a result of closing both the ClockWise and CounterClockWise valves, resulting in a pressure build up in the chambers between push-UP valves. It is not clear how pervasive a problem this is, but I have made changes to the [[Microfluidic chemostat labview program]] to close the CW and CCW sequentially to help the problem.
	#Cells stop producing fluorescent proteins		#Cells stop producing fluorescent proteins
-	~~#O2 transfer in the chip~~
-	#*After growing cells for ~1 week in the reactor the growth rate begins to slow and the cells proceed slowly towards washout. We suspect this is due to oxygen transfer issues in the chip though we are in the process of confirming that as the source of the problem.
	#Control lines		#Control lines
	#*Over time the control lines empty of water. This was observed with green food dye.		#*Over time the control lines empty of water. This was observed with green food dye.

Jason R. Kelly — Tue, 29 May 2007 21:21:29 GMT

←Older revision		Revision as of 21:21, 29 May 2007
Line 40:		Line 40:
	==Posters==		==Posters==
	[[:Image:JK ICSB 2005.pdf\|ICSB 2005 Poster]]		[[:Image:JK ICSB 2005.pdf\|ICSB 2005 Poster]]
		+
		+	==Notebook==
		+	*[[Sortostat/Notebook]] - this is where ongoing work with the sortostat is stored.

	==Researchers==		==Researchers==
	[[Jason Kelly]], [[Josh Michener]], [[Bryan Hernandez]]		[[Jason Kelly]], [[Josh Michener]], [[Bryan Hernandez]]

Jason R. Kelly — Tue, 29 May 2007 21:19:12 GMT

Design

←Older revision		Revision as of 21:19, 29 May 2007
Line 8:		Line 8:
	[[Sortostat/Optimal sorting cutoffs\|Optimal sorting cutoffs]]		[[Sortostat/Optimal sorting cutoffs\|Optimal sorting cutoffs]]

-	[[Sortostat/Design/~~v2.0\|Sortostat~~ v2.0]]	+	[[Sortostat/Design/v2.0]]

	The design of the Sortostat is an extension of the microchemostat design created by Frederick Balagadde. ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15994559&query_hl=1&itool=pubmed_docsum REF])		The design of the Sortostat is an extension of the microchemostat design created by Frederick Balagadde. ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15994559&query_hl=1&itool=pubmed_docsum REF])

Jason R. Kelly — Tue, 29 May 2007 21:10:50 GMT

Design

←Older revision		Revision as of 21:10, 29 May 2007
Line 8:		Line 8:
	[[Sortostat/Optimal sorting cutoffs\|Optimal sorting cutoffs]]		[[Sortostat/Optimal sorting cutoffs\|Optimal sorting cutoffs]]

-	[[Sortostat/~~Chip schematic~~\|~~Chip schematic~~]]	+	[[Sortostat/Design/v2.0\|Sortostat v2.0]]

	The design of the Sortostat is an extension of the microchemostat design created by Frederick Balagadde. ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15994559&query_hl=1&itool=pubmed_docsum REF])		The design of the Sortostat is an extension of the microchemostat design created by Frederick Balagadde. ([http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15994559&query_hl=1&itool=pubmed_docsum REF])

Jason R. Kelly — Tue, 29 May 2007 21:03:58 GMT

Experimental Results

←Older revision		Revision as of 21:03, 29 May 2007
Line 34:		Line 34:

	==Experimental Results==		==Experimental Results==
-	*[[~~SortoStat~~/Experiments~~\|SortoStat Experiments~~]]	+	*[[Sortostat/Experiments/Early experiments]]
-	*[[Sortostat/~~Growth Tests\|SortoStat Growth Tests~~]]	+	*[[Sortostat/Experiments/Brian growth tests]]
	*[[Sortostat/Experiments/Two reactor chip]]		*[[Sortostat/Experiments/Two reactor chip]]

Jason R. Kelly — Tue, 29 May 2007 20:48:21 GMT

Experimental Results

←Older revision		Revision as of 20:48, 29 May 2007
Line 36:		Line 36:
	*[[SortoStat/Experiments\|SortoStat Experiments]]		*[[SortoStat/Experiments\|SortoStat Experiments]]
	*[[Sortostat/Growth Tests\|SortoStat Growth Tests]]		*[[Sortostat/Growth Tests\|SortoStat Growth Tests]]
		+	*[[Sortostat/Experiments/Two reactor chip]]

	==Posters==		==Posters==

Jason R. Kelly — Thu, 18 May 2006 16:29:56 GMT

Known Issues

←Older revision		Revision as of 16:29, 18 May 2006
Line 30:		Line 30:
	#O2 transfer in the chip		#O2 transfer in the chip
	#*After growing cells for ~1 week in the reactor the growth rate begins to slow and the cells proceed slowly towards washout. We suspect this is due to oxygen transfer issues in the chip though we are in the process of confirming that as the source of the problem.		#*After growing cells for ~1 week in the reactor the growth rate begins to slow and the cells proceed slowly towards washout. We suspect this is due to oxygen transfer issues in the chip though we are in the process of confirming that as the source of the problem.
		+	#Control lines
		+	#*Over time the control lines empty of water. This was observed with green food dye.

	==Experimental Results==		==Experimental Results==