Sortostat/Experiments/Brian growth tests

03/12/06

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

• Strain: MC4100
• Device: I6032
• Media: Supplemented M9 + Amp
• Temp: 37C

Chemostat Settings

There are no sorts in this run.

• Dilution Rate: .69/hr
• Clean Chemostat Period: 1.45hr
• Minimum Pumping Time: 30s
• Dead Time per Screen: 20s
• Dead Time per Sort: 47s
• Pump Cycle Period: 400ms

• Actual Cleaning Period: 1.32hr
• Sorting Perdiod: 4.95

• Feeder Fill: 10s
• Wash: 10s
• Clean: 15s
• Pressurize: 5s
• Fluid Pressure: 4.47 psi
• Push-UP: 13.91 psi
• Push-DOWN: 19.7 psi

Results

Catastrophic Failure! Airline blew off actuator and all tank pressure was lost.

Cells look to stabilize at ~100, then the pressure fails and cells diverge from steady state

01/24/06

Purpose: To establish an approximate life expectancy for E.Coli in the sortostat under chemostatic operation.

Experimental Conditions

• Strain: MC4100
• Device: I6032
• Media: Supplemented M9 + Amp
• Temp: 37C

Chemostat Settings

There are no sorts in this run.

• Dilution Rate: Varies, (see graph)
• Clean Chemostat Period: 1/D.R.
• Minimum Pumping Time: Changed from 60s to 50s when the dilution rate changed from 0.4/hr to 0.5/hr
• Dead Time per Screen: 10s
• Dead Time per Sort: 75s
• Pump Cycle Period: 400ms

• Actual Cleaning Period: ?
• Sorting Perdiod: ?

• Feeder Fill: 10s
• Wash: 10s
• Clean: 15s
• Pressurize: 5s
• Fluid Pressure: 5.19 psi
• Push-UP: 15.47 psi
• Push-DOWN: 19.39 psi

Results

Equipment failure

Previous Work

10/09/05

Cell death in long term experiment due to possible O2 depletion