Sortostat/Experiments/Early experiments: Difference between revisions
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This 2 hours at dilution rate of ~.7 hr-1 (~1hr doubling time). | This 2 hours at dilution rate of ~.7 hr-1 (~1hr doubling time). | ||
[[Image:SSchemo.PNG|SteadState?|Early Steady-stateish results]] | [[Image:SSchemo.PNG|SteadState?|Early Steady-stateish results|thumb|500px|left]] | ||
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==Experiment on 06/07/2005== | ==Experiment on 06/07/2005== | ||
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*Strain: [[Genotypes#MC4100|MC4100]] | *Strain: [[Genotypes#MC4100|MC4100]] | ||
*Device: I7101 | *Device: I7101 | ||
*Media: [[M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | *Media: [[Knight:M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | ||
*Temp: 37C | *Temp: 37C | ||
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===Results=== | ===Results=== | ||
[[Image:050607Chemo12hr.PNG|Chemostat Run]] | [[Image:050607Chemo12hr.PNG|Chemostat Run|thumb|500px|left]] | ||
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Looks like hit SS @ ~15,000 cells in the reactor volume ( | Looks like hit SS @ ~15,000 cells in the reactor volume (10nL). This is 1.5*10^9 cells/ml. | ||
[[Sortostat Image Processing]] | [[Sortostat Image Processing]] | ||
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*Strain: [[Genotypes#MC4100|MC4100]] | *Strain: [[Genotypes#MC4100|MC4100]] | ||
*Device: I7101 | *Device: I7101 | ||
*Media: [[M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | *Media: [[Knight:M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | ||
*Temp: 37C | *Temp: 37C | ||
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===Results=== | ===Results=== | ||
[[Image:90hrRunf.PNG]] | [[Image:90hrRunf.PNG|thumb|500px|left]] | ||
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''Darker lines are moving average with 10 time point window.'' | |||
Looked pretty good. Probably should have left longer at D=0.5 (first time) to see where the SS was exactly since it looks like the cell count may still have been decreasing. Cell counts were determined by processing of FL images as shown [[Sortostat Image Processing|here]]. | |||
== Experiment 8/8/1005 to == | |||
===Experimental Conditions for GRwoth Test=== | |||
*Strain: [[E. coli genotypes#MC4100|MC4100]] | |||
*Device: I13602, | |||
*Media: [[Knight:M9 supplemented media|Supplemented M9]] + [[Ampicillin|Amp]] | |||
*Temp: 37C | |||
Dilution Rate: 0.05 | |||
*Clean Chemostat Period: 30hr | |||
*Minimum Pumping Time: 300s | |||
*Dead Time per Screen: 10s | |||
*Dead Time per Sort: 40s | |||
*Pump Cycle Period: 400s | |||
*Actual Cleaning Period: 29.6hr | |||
*Sorting Perdiod: 37min | |||
*Feeder Fill: 10s | |||
*Wash: 10s | |||
*Clean: 15s | |||
*Pressurize: 5s | |||
*Fluid Pressure: 5.8 psi | |||
*Push-UP: 15 psi | |||
*Push-DOWN: 15 psi | |||
Latest revision as of 14:02, 29 May 2007
Early Results
This 2 hours at dilution rate of ~.7 hr-1 (~1hr doubling time).
Experiment on 06/07/2005
Experimental Conditions
- Strain: MC4100
- Device: I7101
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Operating Conditions
- Dilution Rate: 0.3 hr-1
- Clean Chemostat Period: 5hr
- Minimum Pumping Time: 150s
- Dead Time per Screen: 10s
- Dead Time per Sort: 40s
- Pump Cycle Period: 400s
- Actual Cleaning Period: 4.9333hr
- Sorting Perdiod: 6.1667 m
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 5.2 psi
- Push-UP: 15.6 psi
- Push-DOWN: 16.7 psi
Results
Looks like hit SS @ ~15,000 cells in the reactor volume (10nL). This is 1.5*10^9 cells/ml.
Experiment on 6/9 to 6/13/05
Experimental Conditions
- Strain: MC4100
- Device: I7101
- Media: Supplemented M9 + Amp
- Temp: 37C
Chemostat Operating Conditions
- Same as 6/7 experiment.
- Except dilution rate varied from D=0.3 to 0.6 and the min pump period for D=0.6 was set to 50s. Cleaning period and sorting period varied for each dilution rate.
Results
Darker lines are moving average with 10 time point window.
Looked pretty good. Probably should have left longer at D=0.5 (first time) to see where the SS was exactly since it looks like the cell count may still have been decreasing. Cell counts were determined by processing of FL images as shown here.
Experiment 8/8/1005 to
Experimental Conditions for GRwoth Test
- Strain: MC4100
- Device: I13602,
- Media: Supplemented M9 + Amp
- Temp: 37C
Dilution Rate: 0.05
- Clean Chemostat Period: 30hr
- Minimum Pumping Time: 300s
- Dead Time per Screen: 10s
- Dead Time per Sort: 40s
- Pump Cycle Period: 400s
- Actual Cleaning Period: 29.6hr
- Sorting Perdiod: 37min
- Feeder Fill: 10s
- Wash: 10s
- Clean: 15s
- Pressurize: 5s
- Fluid Pressure: 5.8 psi
- Push-UP: 15 psi
- Push-DOWN: 15 psi
