Sortostat/Experiments/Two reactor chip

This is a summary of successful experiments done with the new version of the chip (two reactors).

Feb2006

• What was the date of this experiment
• post image

4/21/07

• post images of %CFP and cell counts

5/29/07

• Media:
  • 0.4% glycerol
  • Amp
  • BSA(2mg/ml)
• Temp: 37C (set to 40.4 on the incubator).
  • What was the offset?
• Fluid: 5.1 psi
• Push down: 21.9 psi
• Push up: 20.2 psi

• Clean period:15.9 min
  1. of sorts / clean: 10
• Number of screens / sort: 2
• Cleaning block length (min): 1
• Pump cycle period (ms): 400
• Ratio history length: 15
• Pump period between screens (sec): 5

• Wash sorting chamber period (ms): 1000
• Media inlet source: center
• Feeder fill period (ms): 1000
• Clean period (ms): 15000
• Pressurize period (ms): 1000

Refocus

• 4465, 4756, 6863, 8375, 9924, 10643, 10814, 11581, 12265, 13087

Flips

• 4888
• 6864 - against YFP
• 9068 - against CFP
• 10324 - against YFP
• 11586 - random sorting
• 13087 - against CFP

7/11/07

7/11/07

• cleaning period = 15.9
• same as 5/29/07, otherwise

7/11/07b

fluid 5.5 pushup: 19.64 pushdown: 21.80

• cleaning period = 14.8
• Focus: 1375, 1984, 2102, 3648, 4068, 5600, 6022, 7502, 8008, 9100, 9718, 9927, 11062, 13036, 13638, 13662, 15358
• 1436 - Started sorting againstYFP
• 9927 - turned off sorting
• 13708 - started sorting againstCFP

9/11/07

• 40 to 60% zig-zag experiment.
  • If possible do pulse selection as well
• cleaning period = 15.9
• same as 5/29/07, otherwise
• Focus and flipped the 2x magnification at #63 (I was seeing cells outside the sorting chamber).

12/2/07

• 1418 - focused
• 3832 - focused for BF images.

12/18/07

• 64 - switched to sorting against CFP, since it looked like they might be growing faster (though too early to tell, really)
• 94 - turned off directional sorting
• 748 - focused
• 752 - directional sorting against CFP
• 1321 - focused
• 3228 - got through >3000 w/o a memory leak crash, as a point of reference. (though PF at 1.2MB)
• Also, note that this chip has been in use since 12/2 (16days), although i did have to reload the pump valves at day 14, b/c one had dried out.