Sortostat/Experiments/Two reactor chip
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This is a summary of successful experiments done with the new version of the chip (two reactors).
Feb2006
- What was the date of this experiment
- post image
4/21/07
- post images of %CFP and cell counts
5/29/07
- Media:
- 0.4% glycerol
- Amp
- BSA(2mg/ml)
- Temp: 37C (set to 40.4 on the incubator).
- What was the offset?
- Fluid: 5.1 psi
- Push down: 21.9 psi
- Push up: 20.2 psi
- Clean period:15.9 min
- of sorts / clean: 10
- Number of screens / sort: 2
- Cleaning block length (min): 1
- Pump cycle period (ms): 400
- Ratio history length: 15
- Pump period between screens (sec): 5
- Wash sorting chamber period (ms): 1000
- Media inlet source: center
- Feeder fill period (ms): 1000
- Clean period (ms): 15000
- Pressurize period (ms): 1000
Refocus
- 4465, 4756, 6863, 8375, 9924, 10643, 10814, 11581, 12265, 13087
Flips
- 4888
- 6864 - against YFP
- 9068 - against CFP
- 10324 - against YFP
- 11586 - random sorting
- 13087 - against CFP
7/11/07
7/11/07
- cleaning period = 15.9
- same as 5/29/07, otherwise
7/11/07b
fluid 5.5 pushup: 19.64 pushdown: 21.80
- cleaning period = 14.8
- Focus: 1375, 1984, 2102, 3648, 4068, 5600, 6022, 7502, 8008, 9100, 9718, 9927, 11062, 13036, 13638, 13662, 15358
- 1436 - Started sorting againstYFP
- 9927 - turned off sorting
- 13708 - started sorting againstCFP
9/11/07
- 40 to 60% zig-zag experiment.
- If possible do pulse selection as well
- cleaning period = 15.9
- same as 5/29/07, otherwise
- Focus and flipped the 2x magnification at #63 (I was seeing cells outside the sorting chamber).
12/2/07
- 1418 - focused
- 3832 - focused for BF images.
12/18/07
- 64 - switched to sorting against CFP, since it looked like they might be growing faster (though too early to tell, really)
- 94 - turned off directional sorting
- 748 - focused
- 752 - directional sorting against CFP
- 1321 - focused
- 3228 - got through >3000 w/o a memory leak crash, as a point of reference. (though PF at 1.2MB)
- Also, note that this chip has been in use since 12/2 (16days), although i did have to reload the pump valves at day 14, b/c one had dried out.