Sosnick:Protocols: Difference between revisions
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==Protein== | |||
===Expression and Purification=== | |||
[[Protein Expression and Purification | Protein Expression]] | |||
[[ | [[Sosnick:Purification of CBP tagged proteins | Calmodulin affinity purification]] | ||
[[ | [[Purification_of_His-tagged_proteins | His-tag purification]] | ||
[[Knight: | [[Knight:Purification_of_MBP-tagged_proteins | Maltose Binding Protein tag purification]] | ||
[[ | [[Purifying Ubiquitin from expression lysates | Purifying Ubiquitin]] | ||
[[ | [[Purifying ctAcP]] | ||
===Chemical Synthesis and Modification=== | |||
[[Free Sulfhydryl Determination]] | |||
[[Solid Phase Peptide Synthesis]] | [[Solid Phase Peptide Synthesis]] | ||
| Line 26: | Line 25: | ||
[[Cyanogen Bromide Cleavage of Ubiquitin]] | [[Cyanogen Bromide Cleavage of Ubiquitin]] | ||
===Analysis=== | |||
[[Sauer:bis-Tris SDS-PAGE, the very best | Bis-tris SDS-PAGE]] | |||
[[Sosnick:Rapid Coomassie Staining]] | |||
==Nucleic Acid== | |||
[[Agarose gel electrophoresis]] | |||
[[Designing primers]] | [[Designing primers]] | ||
[[PCR]] | |||
[[PNK Treatment of DNA Ends]] | |||
[[Phosphatase_treatment_of_linearized_vector | CIPing the vector]] | |||
[[Ethanol precipitation of nucleic acids]] | [[Ethanol precipitation of nucleic acids]] | ||
| Line 37: | Line 49: | ||
[[Site-directed mutagenesis]] | [[Site-directed mutagenesis]] | ||
[[Sauer:Stitching_Genes_by_PCR | Overlap extension PCR]] | |||
==E. coli== | |||
[[Preparing_chemically_competent_cells | Chemically | [[Preparing_chemically_competent_cells | Chemically Competent cells (TSS)]] | ||
[[Electrocompetent cells]] | [[Electrocompetent cells]] | ||
Latest revision as of 11:16, 8 September 2007
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Protein
Expression and Purification
Calmodulin affinity purification
Maltose Binding Protein tag purification
Chemical Synthesis and Modification
Cyanogen Bromide Cleavage of Ubiquitin
Analysis
Sosnick:Rapid Coomassie Staining
Nucleic Acid
Ethanol precipitation of nucleic acids
