Sosnick:Protocols: Difference between revisions
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[[Sosnick: | ==Protein== | ||
===Expression and Purification=== | |||
[[Protein Expression and Purification | Protein Expression]] | |||
[[Sosnick:Purification of CBP tagged proteins | Calmodulin affinity purification]] | |||
[[Purification_of_His-tagged_proteins | His-tag purification]] | |||
[[Knight:Purification_of_MBP-tagged_proteins | Maltose Binding Protein tag purification]] | |||
[[Purifying Ubiquitin from expression lysates | Purifying Ubiquitin]] | |||
[[Purifying ctAcP]] | |||
===Chemical Synthesis and Modification=== | |||
[[Free Sulfhydryl Determination]] | |||
[[Solid Phase Peptide Synthesis]] | |||
[[Cyanogen Bromide Cleavage of Ubiquitin]] | |||
===Analysis=== | |||
[[Sauer:bis-Tris SDS-PAGE, the very best | Bis-tris SDS-PAGE]] | |||
[[Sosnick:Rapid Coomassie Staining]] | |||
==Nucleic Acid== | |||
[[Agarose gel electrophoresis]] | |||
[[Designing primers]] | |||
[[PCR]] | |||
[[PNK Treatment of DNA Ends]] | |||
[[Phosphatase_treatment_of_linearized_vector | CIPing the vector]] | |||
[[Ethanol precipitation of nucleic acids]] | |||
[[Jamesh008:consensus DNA ligation protocol | DNA Ligation]] | |||
[[Site-directed mutagenesis]] | |||
[[Sauer:Stitching_Genes_by_PCR | Overlap extension PCR]] | |||
==E. coli== | |||
[[Preparing_chemically_competent_cells | Chemically Competent cells (TSS)]] | |||
[[Electrocompetent cells]] | |||
Latest revision as of 11:16, 8 September 2007
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Protein
Expression and Purification
Calmodulin affinity purification
Maltose Binding Protein tag purification
Chemical Synthesis and Modification
Cyanogen Bromide Cleavage of Ubiquitin
Analysis
Sosnick:Rapid Coomassie Staining
Nucleic Acid
Ethanol precipitation of nucleic acids
