Sosnick:Protocols

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{{SosnickLab_logo_nav}}
{{SosnickLab_logo_nav}}
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===Protein===
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==Protein==
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[[Sauer:bis-Tris SDS-PAGE, the very best]]
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[[Free Sulfhydryl Determination]]
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===Expression and Purification===
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[[Protein Expression and Purification | Protein Expression]]
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[[Knight:Purification of His-tagged proteins]]
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[[Purification_of_His-tagged_proteins | His-tag purification]]
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[[Knight:Purification of His-tagged proteins/Denaturing]]
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[[Knight:Purification_of_MBP-tagged_proteins | Maltose Binding Protein tag purification]]
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[[Knight:Purification of His-tagged proteins/Denaturing with refolding]]
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[[Purifying Ubiquitin from expression lysates | Purifying Ubiquitin]]
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[[Knight:Purification of His-tagged proteins/Native]]
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[[Protein Expression and Purification]]
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[[Purifying Ubiquitin from expression lysates]]
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[[Purifying ctAcP]]
[[Purifying ctAcP]]
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===Chemical Synthesis and Modification===
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[[Free Sulfhydryl Determination]]
[[Solid Phase Peptide Synthesis]]
[[Solid Phase Peptide Synthesis]]
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[[Cyanogen Bromide Cleavage of Ubiquitin]]
[[Cyanogen Bromide Cleavage of Ubiquitin]]
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===Analysis===
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===Nucleic Acid===
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[[Sauer:bis-Tris SDS-PAGE, the very best | Bis-tris SDS-PAGE]]
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==Nucleic Acid==
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[[Agarose gel electrophoresis]]
[[Designing primers]]
[[Designing primers]]
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[[PCR]]
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[[PNK Treatment of DNA Ends]]
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[[Phosphatase_treatment_of_linearized_vector | CIPing the vector]]
[[Ethanol precipitation of nucleic acids]]
[[Ethanol precipitation of nucleic acids]]
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[[Site-directed mutagenesis]]
[[Site-directed mutagenesis]]
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===E. coli===
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[[Sauer:Stitching_Genes_by_PCR | Overlap extension PCR]]
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==E. coli==
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[[Preparing_chemically_competent_cells | Chemically competent cells]]
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[[Preparing_chemically_competent_cells | Chemically Competent cells (TSS)]]
[[Electrocompetent cells]]
[[Electrocompetent cells]]

Revision as of 13:01, 8 September 2007

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Contents

Protein

Expression and Purification

Protein Expression

His-tag purification

Maltose Binding Protein tag purification

Purifying Ubiquitin

Purifying ctAcP

Chemical Synthesis and Modification

Free Sulfhydryl Determination

Solid Phase Peptide Synthesis

Cyanogen Bromide Cleavage of Ubiquitin

Analysis

Bis-tris SDS-PAGE

Nucleic Acid

Agarose gel electrophoresis

Designing primers

PCR

PNK Treatment of DNA Ends

CIPing the vector

Ethanol precipitation of nucleic acids

DNA Ligation

Site-directed mutagenesis

Overlap extension PCR

E. coli

Chemically Competent cells (TSS)

Electrocompetent cells

Personal tools