User contributions
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- 23:15, 28 November 2009 (hist) (diff) Silver: Lab Meeting Shedule
- 15:19, 19 November 2009 (hist) (diff) Silver: Lab Meeting Shedule
- 08:13, 25 July 2009 (hist) (diff) User:KevinShee (top)
- 21:30, 18 May 2008 (hist) (diff) IGEM:Harvard/2007 (top)
- 21:29, 18 May 2008 (hist) (diff) IGEM:Harvard/2007
- 21:29, 18 May 2008 (hist) (diff) IGEM:Harvard/2007
- 21:28, 18 May 2008 (hist) (diff) IGEM:Harvard/2007
- 21:28, 18 May 2008 (hist) (diff) IGEM:Harvard/2007
- 21:27, 18 May 2008 (hist) (diff) IGEM:Harvard/2007
- 16:53, 29 November 2007 (hist) (diff) IGEM:Harvard/2007/Kevin's 91r Notebook (top)
- 16:32, 26 November 2007 (hist) (diff) N IGEM:Harvard/2007/Kevin's 91r Notebook (New page: *Step 1: Overnight culture from Perry's frozen stock of bacteria with LppOmpA plasmid. *Step 2: Plasmid prep of LppOmpA plasmid. *Step 3: Purification of plasmid. *Step 4: Design primers ...)
- 16:22, 26 November 2007 (hist) (diff) User:KMagic246 (top)
- 17:42, 30 October 2007 (hist) (diff) IGEM:Harvard/2007/FinalPresentation
- 17:41, 30 October 2007 (hist) (diff) N Image:Harvardigem07finalppt v23.ppt (top)
- 13:00, 23 August 2007 (hist) (diff) IGEM:Harvard/2007
- 15:49, 21 August 2007 (hist) (diff) IGEM:Harvard/2007
- 11:33, 17 August 2007 (hist) (diff) N IGEM:Harvard/2007/To Do List (New page: #PCR Amplify AIDA1 template #Digest PCR product/vectors #Ligate digested products #Transform ligation product plate and add selection to liquid media #Run cells in columns #Design T-shirt ...) (top)
- 11:32, 17 August 2007 (hist) (diff) IGEM:Harvard/2007
- 11:31, 17 August 2007 (hist) (diff) IGEM:Harvard/2007
- 11:20, 17 August 2007 (hist) (diff) N IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 9 (New page: ===Transformation of OMPA loop his and strep (8/14/07)=== #Add 1 µl of the DNA solution directly to the cells. Stir gently to mix. #Place the tubes on ice for a little more than 5 minute...) (top)
- 11:15, 17 August 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol (top)
- 16:52, 16 August 2007 (hist) (diff) N IGEM:Harvard/2007/Pictures/Week 9 (New page: 250px 250px<br> 250px 250px<br> 250px [[Image:Wee...) (top)
- 16:52, 16 August 2007 (hist) (diff) N Image:Week9-10.jpg (top)
- 16:51, 16 August 2007 (hist) (diff) N Image:Week9-9.jpg (top)
- 16:51, 16 August 2007 (hist) (diff) N Image:Week9-8.jpg (top)
- 16:50, 16 August 2007 (hist) (diff) N Image:Week9-7.jpg (top)
- 16:50, 16 August 2007 (hist) (diff) N Image:Week9-6.jpg (top)
- 16:49, 16 August 2007 (hist) (diff) N Image:Week9-5.jpg (top)
- 16:48, 16 August 2007 (hist) (diff) N Image:Week9-4.jpg (top)
- 16:48, 16 August 2007 (hist) (diff) N Image:Week9-3.jpg (top)
- 16:47, 16 August 2007 (hist) (diff) N Image:Week9-2.jpg (top)
- 16:47, 16 August 2007 (hist) (diff) N Image:Week9-1.jpg (top)
- 16:45, 16 August 2007 (hist) (diff) IGEM:Harvard/2007/Pictures/Week 6 (top)
- 16:45, 16 August 2007 (hist) (diff) N IGEM:Harvard/2007/Pictures/Week 6 (New page: 250px 250px 250px 250px 250px [[Image:Week6-6.jpg...)
- 16:45, 16 August 2007 (hist) (diff) N Image:Week6-7.jpg (top)
- 16:44, 16 August 2007 (hist) (diff) N Image:Week6-6.jpg (top)
- 16:44, 16 August 2007 (hist) (diff) N Image:Week6-5.jpg (top)
- 16:43, 16 August 2007 (hist) (diff) N Image:Week6-4.jpg (top)
- 16:43, 16 August 2007 (hist) (diff) N Image:Week6-3.jpg (top)
- 16:43, 16 August 2007 (hist) (diff) N Image:Week6-2.jpg (top)
- 16:41, 16 August 2007 (hist) (diff) N Image:Week6-1.jpg (top)
- 10:55, 13 August 2007 (hist) (diff) IGEM:Harvard/2007
- 10:55, 13 August 2007 (hist) (diff) IGEM:Harvard/2007
- 10:54, 13 August 2007 (hist) (diff) IGEM:Harvard/2007
- 10:53, 13 August 2007 (hist) (diff) IGEM:Harvard/2007
- 10:40, 13 August 2007 (hist) (diff) IGEM:Harvard/2007/Plates (→MACS Cell Sort with AIDA Sender Constructs) (top)
- 15:23, 11 August 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 8 (→MACS Assay 2- his and strep AIDA sender cells (08/05/07))
- 15:20, 11 August 2007 (hist) (diff) IGEM:Harvard/2007/Plates (→MACS Cell Sort with AIDA Construct-All Induced)
- 13:20, 10 August 2007 (hist) (diff) N IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 8 (New page: ===MACS Assay 1- his and strep AIDA sender cells (08/05/07)=== Purpose: Testing the AIDA sender cell constructs. #OD equilibriated to 0.3 for induced #Followed [[IGEM:Harvard/2007/Protoc...)
- 13:17, 10 August 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 10:25, 7 August 2007 (hist) (diff) IGEM:Harvard/2007/Plates (→MACS and FACS Cell Sort with AIDA Construct)
- 10:25, 7 August 2007 (hist) (diff) IGEM:Harvard/2007/Plates
- 10:23, 7 August 2007 (hist) (diff) IGEM:Harvard/2007/Plates (→MACS Cell Sort with AIDA Construct-All Induced)
- 10:22, 7 August 2007 (hist) (diff) IGEM:Harvard/2007/Plates
- 16:16, 5 August 2007 (hist) (diff) IGEM:Harvard/2007
- 14:34, 5 August 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 7 (→Digest AIDA1 in pET29b+ for library insertion (8/2/07)) (top)
- 14:32, 5 August 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 7 (→MACS Assay - his and strep AIDA cells with smaller concentration (7/31/07))
- 13:09, 2 August 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 7 (→Results of Electroporation 7/31/07)
- 10:27, 1 August 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 7
- 10:27, 1 August 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 7
- 13:49, 30 July 2007 (hist) (diff) N IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 7 (New page: ===Gel Purification of AIDA Digest (07/30/07)=== #Add 150 ml 1x TBS #Add 1.8 g of agarose #Heat to a boil, let cool to a warm/hot temperature. #Add 1.5 ul EtBr #Pour into gel and wait unti...)
- 13:47, 30 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 10:41, 30 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 6 (→Run E-Gel with PCR Products (07/26/07)) (top)
- 10:40, 30 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 6 (→Run E-Gel with PCR Products (07/26/07))
- 10:39, 30 July 2007 (hist) (diff) IGEM:Harvard/2007/Gels (→7/09 Egel, Alex) (top)
- 10:39, 30 July 2007 (hist) (diff) IGEM:Harvard/2007/Gels (→7/09 Egel, Alex)
- 10:38, 30 July 2007 (hist) (diff) IGEM:Harvard/2007/Gels (→7/09 Egel, Alex)
- 10:37, 30 July 2007 (hist) (diff) N Image:KevinMikeGel.jpg (top)
- 10:36, 30 July 2007 (hist) (diff) IGEM:Harvard/2007/Gels
- 10:34, 30 July 2007 (hist) (diff) N Image:KevinMikePCR.jpg (top)
- 15:08, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 6 (→Run E-Gel with PCR Products (07/26/07))
- 15:08, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 6 (→PCR Reaction with AIDA construct)
- 14:31, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 6 (→PCR Reaction with AIDA construct)
- 14:28, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 6
- 12:39, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Video Clips
- 12:38, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Video Clips
- 12:38, 26 July 2007 (hist) (diff) N Image:IGEM Lab Tour.wmv (top)
- 11:06, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Video Clips
- 10:56, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Video Clips
- 10:55, 26 July 2007 (hist) (diff) N Image:The Fly.MPG (top)
- 10:39, 26 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 6
- 16:25, 24 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 6
- 10:43, 24 July 2007 (hist) (diff) IGEM:Harvard/2007/Plates (→MACS and FACS Cell Sort with AIDA Construct)
- 13:43, 23 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 5 (→FACS and MACS with AIDA Construct Results (7/22/07)) (top)
- 13:41, 23 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 5 (→FACS Cell Sorting Results (7/21/07))
- 13:38, 23 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 13:35, 23 July 2007 (hist) (diff) IGEM:Harvard/2007/Plates (→MACS Cell Sort with AIDA Construct)
- 13:35, 23 July 2007 (hist) (diff) IGEM:Harvard/2007/Plates (→FACS Cell Sort with AIDA Construct)
- 13:34, 23 July 2007 (hist) (diff) IGEM:Harvard/2007/Plates
- 13:30, 23 July 2007 (hist) (diff) IGEM:Harvard/2007/Plates
- 11:51, 21 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 11:51, 21 July 2007 (hist) (diff) N IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 5 (New page: ===MACS Assay w/o RFP cells - BL21DE3 cells vs his and strep OmpA1 cells (7/17/07)=== Purpose: To test binding with OmpA1 cells with a lower concentration of cells, using BL21DE3 with his...)
- 11:51, 21 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 11:50, 21 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 11:50, 21 July 2007 (hist) (diff) N IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 4 (New page: ===pre-MACS work done by Mike (07/09/07)=== #Grew overnight cultures #1-5 dilution #Grew to 0.6-0.7 OD and induced with IPTG for 3 hours. -------------- ===MACS Column for separation of ...) (top)
- 11:50, 21 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 11:49, 21 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 11:49, 21 July 2007 (hist) (diff) N IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol/Week 3 (New page: ===MACS with strep and his plus cherry/red bacteria (07/02/07)=== ---------- ===Run the MACS with the cherry/red bacteria incorporated to see if separation occurs (07/03/07)=== #Followe...) (top)
- 11:49, 21 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
- 11:49, 21 July 2007 (hist) (diff) IGEM:Harvard/2007/Laboratory Notebooks/Bacterial Targeting Protocol
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