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- 14:04, 5 March 2013 (hist) (diff) Sean Lauber:MTT Cell Proliferation Assay (top)
- 17:35, 25 January 2013 (hist) (diff) N Sean Lauber:Thawing Mammalian Cells (New page: 1. Remove a vial from liquid nitrogen and transport (quickly) on ice (best is dry ice but ice is okay) 2. Thaw the vial in a 37°C water bath by constantly swirling the vial in the bath (...) (top)
- 17:27, 25 January 2013 (hist) (diff) Sean Lauber:Culturing Lewis Lung Carcinoma Cells (top)
- 17:27, 25 January 2013 (hist) (diff) Sean Lauber:Culturing Lewis Lung Carcinoma Cells
- 11:59, 25 January 2013 (hist) (diff) Sean Lauber:Adenovirus infection (in vitro) (top)
- 11:59, 25 January 2013 (hist) (diff) Sean Lauber:Adenovirus infection (in vitro)
- 14:28, 11 December 2012 (hist) (diff) User:Sean E. Lauber (top)
- 13:16, 11 December 2012 (hist) (diff) N Sean Lauber:Culturing of Primary Macrophages (New page: This procedure describes how to culture macrophages derived from from the broncho-alveolar lavage. However, this can be applied to any source of cells where you'd like to isolate an adhere...) (top)
- 12:57, 11 December 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 12:57, 11 December 2012 (hist) (diff) Sean Lauber:Culturing Lewis Lung Carcinoma Cells
- 12:43, 11 December 2012 (hist) (diff) N Sean Lauber:Culturing Lewis Lung Carcinoma Cells (New page: ==Thawing the culture== 1. Thaw a vial of 3 million cells/ml in a 37°C water bath 2. Add 1 ml of PBS and add this 2 ml to 8 ml of PBS 3. Spin at 1000 rpm for 3 min to pellet 4. Discar...)
- 12:14, 11 December 2012 (hist) (diff) Sean Lauber:Adenovirus infection (in vitro)
- 15:50, 10 December 2012 (hist) (diff) N Sean Lauber:Adenovirus infection (in vitro) (New page: This procedure is optimized for using 96-well plates (0.32 cm^2). 1. Seed 2000 cells per well 2. Incubate overnight at 37°C/5% CO2. 3. Wash cells with 100 ul of PBS two times. 4. Add ...)
- 15:13, 10 December 2012 (hist) (diff) User:Sean E. Lauber (→Mammalian Cell Culture)
- 14:03, 10 December 2012 (hist) (diff) Sean Lauber:MTT Cell Proliferation Assay
- 13:13, 10 December 2012 (hist) (diff) N Sean Lauber:MTT Cell Proliferation Assay (New page: The following protocol has been optimized for growing lewis lung carcinoma (LLC) cells over a 2 day period. However I have been able to grow the cells over a 3 day period but this was less...)
- 12:54, 7 December 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer (top)
- 11:58, 6 November 2012 (hist) (diff) User:Sean E. Lauber (→Useful links)
- 11:54, 6 November 2012 (hist) (diff) User:Sean E. Lauber (→Useful links)
- 11:39, 6 November 2012 (hist) (diff) User:Sean E. Lauber
- 17:42, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II (→Procedure) (top)
- 17:34, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 17:34, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 17:34, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 17:32, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 17:30, 5 November 2012 (hist) (diff) User:Sean E. Lauber (→Education)
- 17:29, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan) (top)
- 17:29, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 17:23, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 17:04, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 17:00, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:55, 5 November 2012 (hist) (diff) Image:Template qPCR.xlsx (uploaded a new version of "Image:Template qPCR.xlsx") (top)
- 16:50, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:45, 5 November 2012 (hist) (diff) Image:Template qPCR.xlsx (uploaded a new version of "Image:Template qPCR.xlsx")
- 16:08, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 16:05, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 16:47, 1 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:45, 1 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:44, 1 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:44, 1 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:42, 1 November 2012 (hist) (diff) N Image:Template qPCR.xlsx
- 16:56, 31 October 2012 (hist) (diff) User:Sean E. Lauber (→Technical guides)
- 16:56, 31 October 2012 (hist) (diff) User:Sean E. Lauber (→Links)
- 15:51, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting) (top)
- 15:51, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:49, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:31, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:31, 30 October 2012 (hist) (diff) N Image:Differential4.jpg (top)
- 15:29, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:28, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:27, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:11, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:10, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:09, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:08, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:07, 30 October 2012 (hist) (diff) N Image:Differential3.jpg (top)
- 15:04, 30 October 2012 (hist) (diff) N Image:Differential2.jpg (top)
- 15:03, 30 October 2012 (hist) (diff) N Image:Differential1.jpg (top)
- 14:32, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 14:31, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 14:31, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 14:29, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining
- 14:18, 30 October 2012 (hist) (diff) N Sean Lauber:Differential cell staining (New page: To differentially stain the cytocentrifuge smears (from BAL), the Hema3 reagent is used (Fisher, 23-123-869). Protocol for staining (each dip is done for 1 second): 1. Dip one slide...)
- 14:10, 30 October 2012 (hist) (diff) User:Sean E. Lauber (→Animal Work)
- 10:46, 26 October 2012 (hist) (diff) Sean Lauber:Heat maps in Excel (top)
- 10:44, 26 October 2012 (hist) (diff) Sean Lauber:Heat maps in Excel
- 10:38, 26 October 2012 (hist) (diff) N Sean Lauber:Heat maps in Excel (New page: Use the following macro: Sub heatmap() Dim myrange As Range Dim colchoice As Integer Set myrange = Application.InputBox("Select a range of cells", rangetocheck, , , , , , 8) For...)
- 10:36, 26 October 2012 (hist) (diff) User:Sean E. Lauber (→Technical)
- 14:40, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping (top)
- 09:38, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:36, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:36, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:19, 16 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus (top)
- 14:50, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:20, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:18, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:16, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:12, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:09, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 10:58, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 10:58, 15 October 2012 (hist) (diff) N Sean Lauber:Isotype ELISA against Adenovirus (New page: Want to determine what antibody isotypes are directed against a specific antigen (in my case it was adenovirus). Wash buffer (10X): 800 ml dH2O 24.23 g Tris 87.66 g NaCl 3.73 g KC...)
- 10:54, 15 October 2012 (hist) (diff) Sean Lauber:Generating Adenovirus Lysate (top)
- 10:54, 15 October 2012 (hist) (diff) N Sean Lauber:Generating Adenovirus Lysate (New page: 1. Hela cells were plated onto a T150 (make 2 plates) 2. When confluent, determine the concentration of cells from the second vessel 3. Wash the cells 3x with PBS, remove the media 4. Infe...)
- 10:54, 15 October 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 10:36, 15 October 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 14:48, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:21, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:16, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:12, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:09, 21 September 2012 (hist) (diff) N Sean Lauber:Kras G12D Genotyping (New page: This protocol makes use of the DirectPCR mouse tail lysis reagent (Viagen, cat 102-T) to obtain template and the hot start Platinum Taq (Invitrogen, cat 10966-026). 1 mm tail ends are co...)
- 12:00, 18 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 13:07, 17 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 11:54, 13 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 12:15, 7 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:49, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:36, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:35, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:35, 13 August 2012 (hist) (diff) N Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer (New page: For 5 animals: 1. Thaw a single vial of 1,000,000 cells 2. Add to 9 ml of warmed 10% FBS DMEM 3. Spin at 10,000 RPM for 3 min 4. Discard supernatant 5. Resuspend to 10 ml 6. Plate this vol...)
- 12:08, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development (top)
- 11:51, 11 July 2012 (hist) (diff) N Image:ELISA1.JPG (top)
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