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- 16:58, 5 February 2010 (hist) (diff) Undergraduate BE Board
- 16:50, 5 February 2010 (hist) (diff) Undergraduate BE Board
- 16:49, 5 February 2010 (hist) (diff) N Image:Course 20 Restricted Electives Guide.pdf (top)
- 16:47, 5 February 2010 (hist) (diff) Undergraduate BE Board
- 16:44, 5 February 2010 (hist) (diff) Undergraduate BE Board
- 19:23, 27 October 2009 (hist) (diff) Undergraduate BE Board:IBE Regional Conference Information
- 19:50, 26 September 2009 (hist) (diff) 20.309:Presentations
- 19:46, 26 September 2009 (hist) (diff) 20.309:Presentations
- 16:23, 17 September 2009 (hist) (diff) Undergraduate BE Board
- 22:27, 16 March 2009 (hist) (diff) Undergraduate BE Board:Minutes - 03/16/09 Minutes (top)
- 22:26, 16 March 2009 (hist) (diff) Undergraduate BE Board:Minutes - 03/16/09 Minutes
- 16:59, 8 October 2008 (hist) (diff) Undergraduate BE Board
- 16:51, 8 October 2008 (hist) (diff) Image:BE 2008 T-shirt Colors.jpg (uploaded a new version of "Image:BE 2008 T-shirt Colors.jpg") (top)
- 16:49, 8 October 2008 (hist) (diff) Image:BE 2008 T-shirt Colors.jpg (uploaded a new version of "Image:BE 2008 T-shirt Colors.jpg")
- 16:48, 8 October 2008 (hist) (diff) Image:BE 2008 T-shirt Colors.jpg (uploaded a new version of "Image:BE 2008 T-shirt Colors.jpg")
- 16:48, 8 October 2008 (hist) (diff) Undergraduate BE Board:Fall'08 T-shirts (→The Design)
- 16:47, 8 October 2008 (hist) (diff) Undergraduate BE Board:Fall'08 T-shirts
- 16:46, 8 October 2008 (hist) (diff) Undergraduate BE Board:Fall'08 T-shirts
- 16:44, 8 October 2008 (hist) (diff) Undergraduate BE Board:Fall'08 T-shirts (→Pre-Order Your Shirt)
- 16:19, 8 October 2008 (hist) (diff) N Image:BE 2008 T-shirt Colors.jpg
- 16:18, 8 October 2008 (hist) (diff) Undergraduate BE Board:Fall'08 T-shirts (→The Design)
- 16:14, 8 October 2008 (hist) (diff) N Image:BE T-shirt Colors.jpg (top)
- 16:14, 8 October 2008 (hist) (diff) Undergraduate BE Board:Fall'08 T-shirts (→The Winning Design)
- 16:10, 8 October 2008 (hist) (diff) Undergraduate BE Board:Fall'08 T-shirts
- 18:32, 10 September 2008 (hist) (diff) Undergraduate BE Board:Fall'08 T-shirts (Removing all content from page)
- 18:31, 10 September 2008 (hist) (diff) N Undergraduate BE Board:Fall'08 T-shirts (New page: {{Template:BE_Boardnonflash}} {| |- valign="top" |width=740px class="MainPageBG" style="border: 5px solid #2D9258; color: #000000; background-color: #FFFFFF"| <div style="padding: .4em ....)
- 18:30, 10 September 2008 (hist) (diff) Undergraduate BE Board
- 00:23, 26 February 2008 (hist) (diff) Undergraduate BE Board:Calendar of Events (→Full Year)
- 00:23, 26 February 2008 (hist) (diff) N Undergraduate BE Board:Calendar of Events (New page: <calendar> name=Calendar:<Name> view=days days=1 date=yesterday format=%name/%year-%m-%d formattitle="%M %day, %year (%l) (Yesterday)" skipempty=1 weekformat=list </calendar> <calendar> n...)
- 00:22, 26 February 2008 (hist) (diff) Undergraduate BE Board
- 01:10, 5 February 2008 (hist) (diff) N Undergraduate BE Board:Minutes - 2/4/08 Minutes (New page: {{Template:Undergrad BE Board}} <div style="padding: 10px; width: 720px; border: 5px solid #2D9258;"> '''BE UG Board Meeting <br> February 4, 2008 <br> '''4pm 56-614 <br> Attendance: Da...)
- 00:49, 5 February 2008 (hist) (diff) Undergraduate BE Board:Meetings (→<font color=#2D9258>2008</font>)
- 17:34, 4 February 2008 (hist) (diff) Undergraduate BE Board:Meetings (→<font color=#2D9258>2008</font>)
- 02:02, 30 January 2008 (hist) (diff) N Undergraduate BE Board:Agendas - 2/04/08 Agenda (New page: {{Template:Undergrad BE Board}} <div style="padding: 10px; width: 720px; border: 5px solid #2D9258;"> '''BE UG Board Meeting February 4th 2008''' 56-614 4:00pm #KICK-OFF EVENT #*Mike –...)
- 01:59, 30 January 2008 (hist) (diff) Undergraduate BE Board:Meetings (→<font color=#2D9258>2008</font>)
- 01:57, 30 January 2008 (hist) (diff) Undergraduate BE Board:Minutes - 1/29/08 Minutes
- 01:31, 30 January 2008 (hist) (diff) Undergraduate BE Board:Minutes - 1/24/08 Minutes
- 01:30, 30 January 2008 (hist) (diff) N Undergraduate BE Board:Minutes - 1/29/08 Minutes (New page: {{Template:Undergrad BE Board}} <div style="padding: 10px; width: 720px; border: 5px solid #2D9258;"> '''BE UG Board Meeting <br> January 29, 2008 <br> '''7:30pm Cosi <br> Attendance: S...)
- 01:29, 30 January 2008 (hist) (diff) Undergraduate BE Board:Minutes - 1/24/08 Minutes
- 01:20, 30 January 2008 (hist) (diff) Undergraduate BE Board:Meetings (→<font color=#2D9258>2008</font>)
- 21:39, 25 November 2007 (hist) (diff) 20.109(F07): ECD experimental variations (→W/F lab)
- 11:34, 17 November 2007 (hist) (diff) User:Bernice (→Module 3) (top)
- 11:33, 17 November 2007 (hist) (diff) User:Bernice (→Module 3)
- 11:33, 17 November 2007 (hist) (diff) User:Bernice (→Module 3)
- 11:32, 17 November 2007 (hist) (diff) User:Bernice (→Module 3)
- 11:30, 17 November 2007 (hist) (diff) User:Bernice (→Module 3)
- 08:39, 17 November 2007 (hist) (diff) User:Bernice
- 13:42, 13 November 2007 (hist) (diff) Talk:20.109(F07): Phage nanowires (→W/F TEM schedule)
- 06:56, 31 October 2007 (hist) (diff) 20.109(F07): Module 2 oral presentations (→siRNA validation and applications)
- 06:55, 31 October 2007 (hist) (diff) 20.109(F07): Module 2 oral presentations (→siRNA validation and applications)
- 15:19, 19 October 2007 (hist) (diff) N Module 2, Day 3 (New page: ==Part 1 Pictures== *Image:Mod2Day3Part1-1a *Image:Mod2Day3Part1-2b *Image:Mod2Day3Part1-3a *Image:Mod2Day3Part1-4a *Image:Mod2Day3Part1-5b *Image:Mod2Day3Part1-6a) (top)
- 15:18, 19 October 2007 (hist) (diff) User:Crystal Mao (→Homework)
- 15:17, 19 October 2007 (hist) (diff) N Image:Mod2Day3Part1-6a (top)
- 15:16, 19 October 2007 (hist) (diff) N Image:Mod2Day3Part1-5b (top)
- 15:16, 19 October 2007 (hist) (diff) N Image:Mod2Day3Part1-4a (top)
- 15:15, 19 October 2007 (hist) (diff) N Image:Mod2Day3Part1-3a (top)
- 15:08, 19 October 2007 (hist) (diff) N Image:Mod2Day3Part1-2b (top)
- 15:07, 19 October 2007 (hist) (diff) N Image:Mod2Day3Part1-1a (top)
- 15:05, 19 October 2007 (hist) (diff) Day3 (→Part 1 Pictures) (top)
- 15:05, 19 October 2007 (hist) (diff) N Day3 (New page: ==Part 1 Pictures== Image:Mod2Day3Part1-1a Image:Mod2Day3Part1-2b Image:Mod2Day3Part1-3a Image:Mod2Day3Part1-4a Image:Mod2Day3Part1-5b Image:Mod2Day3Part1-6a)
- 15:02, 19 October 2007 (hist) (diff) User:Bernice (→Contact Info)
- 09:54, 26 September 2007 (hist) (diff) Day 4 (top)
- 09:53, 26 September 2007 (hist) (diff) N Day 4 (New page: 1. {|</center> |- !Transformation !Expected Results !Actual Results !Transformation Efficiency !Explanations |- |Positive Control (pUC18) |most colonies |300 colonies |(300 colonies)/(5 n...)
- 09:24, 26 September 2007 (hist) (diff) User:Bernice (→Day 3)
- 15:38, 25 September 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-9-25 (→Gel Electrophoresis) (top)
- 15:19, 25 September 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-9-25 (→Gel Electrophoresis)
- 15:18, 25 September 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-9-25
- 14:40, 25 September 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-9-25 (→Double Digests)
- 14:31, 25 September 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-9-25 (New page: ====Double Digests==== <pre> 8 µl J04630 0.5 µl BSA 5µl NEB3 Buffer 1µl XbaI 1µl PstI 34.5µl H20 --------------- 50µl Total </pre> <pre> 9 µl J04650 0.5 µl BSA 5µl EcoRI Buffer...)
- 09:38, 21 September 2007 (hist) (diff) Day 3 (top)
- 09:38, 21 September 2007 (hist) (diff) Day 3
- 09:37, 21 September 2007 (hist) (diff) User:Bernice (→Day 3)
- 09:31, 21 September 2007 (hist) (diff) User:Bernice (→Day 3)
- 16:34, 20 September 2007 (hist) (diff) Day 3
- 16:13, 20 September 2007 (hist) (diff) N Day 3 (New page: 2. Outcome #1, no colonies on any plate: This would suggest that the transformation was unsuccessful, because even the positive control with the un-engineered M13K07 DNA failed to grow in...)
- 15:56, 20 September 2007 (hist) (diff) User:Bernice (→Module 1)
- 11:49, 19 September 2007 (hist) (diff) Day 2 (top)
- 09:49, 19 September 2007 (hist) (diff) N Day 2 (New page: 3.)
- 09:49, 19 September 2007 (hist) (diff) User:Bernice
- 11:54, 14 September 2007 (hist) (diff) Bernice:superM13 (top)
- 11:53, 14 September 2007 (hist) (diff) Bernice:superM13
- 11:34, 14 September 2007 (hist) (diff) Bernice:superM13
- 11:19, 14 September 2007 (hist) (diff) Bernice:superM13
- 09:41, 14 September 2007 (hist) (diff) Bernice:superM13
- 08:51, 14 September 2007 (hist) (diff) Bernice:superM13
- 08:48, 14 September 2007 (hist) (diff) Bernice:superM13
- 08:41, 14 September 2007 (hist) (diff) Bernice:superM13
- 08:38, 14 September 2007 (hist) (diff) User:Bernice (→Day 1)
- 08:38, 14 September 2007 (hist) (diff) User:Bernice (→Day 1)
- 16:09, 13 September 2007 (hist) (diff) User:Bernice (→Day 1)
- 16:08, 13 September 2007 (hist) (diff) User:Bernice (→Day 1)
- 16:08, 13 September 2007 (hist) (diff) User:Bernice (→20.109 Fall 2007)
- 15:29, 13 September 2007 (hist) (diff) Bernice:superM13
- 15:29, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:52, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:51, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:50, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:49, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:48, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:46, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:42, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:17, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:17, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:15, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:15, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:13, 13 September 2007 (hist) (diff) Bernice:superM13
- 14:09, 13 September 2007 (hist) (diff) Bernice:superM13 (→Re-engineering the M13K07 Genome)
- 14:04, 13 September 2007 (hist) (diff) N Bernice:superM13 (New page: ==Re-engineering the M13K07 Genome== {| |- |Gene |Base Pairs |Suggested Changes |- | | | |- | | | |- | | | |- | | | |- | | | |- | | | |- | | | |- | | | |- | | | |- | | | |- | | | |- | | |...)
- 13:58, 13 September 2007 (hist) (diff) User:Bernice (→Day 1)
- 13:57, 13 September 2007 (hist) (diff) User:Bernice (→20.109 Fall 2007)
- 13:55, 13 September 2007 (hist) (diff) User:Bernice (→Contact Info)
- 13:54, 13 September 2007 (hist) (diff) User:Bernice
- 13:53, 13 September 2007 (hist) (diff) User:Bernice
- 13:53, 13 September 2007 (hist) (diff) User:Bernice (→Contact Info)
- 13:51, 13 September 2007 (hist) (diff) User:Bernice (→20.109 Fall 2007)
- 13:50, 13 September 2007 (hist) (diff) User:Bernice
- 16:44, 12 September 2007 (hist) (diff) Talk:20.109(F07):Start-up genome engineering (→W/F lab)
- 16:43, 12 September 2007 (hist) (diff) N Image:Day 1, Part 4.doc (top)
- 16:42, 12 September 2007 (hist) (diff) Talk:20.109(F07):Start-up genome engineering (→W/F lab)
- 04:33, 12 September 2007 (hist) (diff) User:Bernice (→Contact Info)
- 04:31, 12 September 2007 (hist) (diff) User:Bernice
- 04:17, 12 September 2007 (hist) (diff) 20.109(F07):People (→Wednesday/Friday lab section)
- 04:11, 12 September 2007 (hist) (diff) 20.109(F07):People (→Wednesday/Friday lab section)
- 15:14, 26 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-26 (New page: ===Agenda=== *Glycerol Stocked/Miniprepped pTO40 and Q04510 *Digestions: *Ligations:) (top)
- 15:12, 26 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-25 (New page: ===Agenda=== *Miniprepped LC's of transformations *picked colonies/made LC's of yesterday's plates for 8/23 transformations) (top)
- 17:10, 25 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-24 (New page: ===Agenda=== *Pick colonies, make LC's of 8/23 transformations :*unable to pick from 2 plates (pTO40 was a smear...transformed with too much DNA?) --> replated those *Make new Kan plates *...) (top)
- 14:00, 23 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-22 (→Wrapped Up Making iGEM Antibiotic Stocks) (top)
- 11:39, 23 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-22 (→Agenda)
- 15:34, 22 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-22
- 15:23, 22 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-22 (→Agenda)
- 15:21, 22 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-22 (New page: ===Agenda=== *Clean out fridge: Threw out contaminated/overgrown plates, added fresh LB to LC's *Made glycerol stock of F2620+B0034+CPX+B0014 (for B-r cells cPCR6-1 and cPCR8-5, for BL21 c...)
- 14:57, 22 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-21 (New page: Agenda: *Replated Transformations from last week (I14032+B0034+CPX+B0014 in 1AC3, Bl21-e) because old plates potentially compromised from fridge contamination before we could LC them *Made...) (top)
- 14:45, 22 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-20 (New page: Agenda: Background Research on Mer Operon) (top)
- 00:35, 17 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16 (→Ligation Mixtures)
- 00:34, 17 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16 (→Nanodrop of Double Digests(BH))
- 23:41, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16
- 21:11, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16 (→DD I14032.B0034 Mixture)
- 21:10, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16
- 15:54, 14 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14 (→Ligations - Take 2)
- 11:34, 14 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14 (→Sequencing of Standard Assembled Parts)
- 11:33, 14 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14 (→Ligations - Take 2)
- 11:33, 14 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14 (→Double Digestion)
- 11:00, 14 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14 (→Sequencing of Standard Assembled Parts)
- 10:30, 14 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-14 (New page: ===Sequencing of Standard Assembled Parts=== :*CPX+B0014: Sample 1: okay except single substitution towards end of CPX sequence, Samples 3-4 successful :*R0051+B0034: Samples 1&3 failed to...)
- 22:25, 12 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-12 (→Removed Transformation Plates from 37C - Colony Count)
- 22:25, 12 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-12 (→Removed Transformation Plates from 37C - Colony Count)
- 22:24, 12 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-12 (→Removed Transformation Plates from 37C - Colony Count)
- 22:23, 12 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-12 (→Removed Transformation Plates from 37C - Colony Count)
- 22:22, 12 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-12 (New page: ===Removed Transformation Plates from 37C - Colony Count=== <pre> Plate Plate Marker Count Notes insert only Cm ...)
- 00:04, 12 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-6
- 18:39, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-11
- 18:36, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-11
- 18:25, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-11 (→Agenda)
- 18:00, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10
- 17:58, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10 (→Blocking/ Antibodies)
- 17:58, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10
- 13:08, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10
- 13:03, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10
- 12:56, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10
- 12:55, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10
- 12:54, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10
- 12:54, 11 August 2007 (hist) (diff) N Image:Newproject5.jpg (top)
- 12:53, 11 August 2007 (hist) (diff) N Image:Newproject4.jpg (top)
- 15:40, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-7
- 10:02, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-4 (top)
- 10:01, 7 August 2007 (hist) (diff) N Image:8.4 Digest Gel.tif (top)
- 10:01, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-4 (→Double Digests!!!)
- 09:59, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-3 (→Gel Electrophoresis of Digestions) (top)
- 09:59, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-3 (→Gel Electrophoresis of Digestions)
- 09:55, 7 August 2007 (hist) (diff) N Image:8.3 Digest gel.jpg (top)
- 09:55, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-3
- 09:53, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-4
- 09:50, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-4
- 12:59, 4 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-4
- 12:59, 4 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-4 (New page: <pre> 5.3 µl I14032 ( 188.4 ng/µl) 0.5 µl BSA 5 µl NEB2 Buffer 1 µl SpeI 1 µl PstI 37.7 µl H20 -------------------------- 50 µl Total 42.5 µl ...)
- 14:51, 3 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-3
- 12:39, 3 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-3
- 12:39, 3 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-3 (→Took Out LC's of I14032, B0014, R0051)
- 12:38, 3 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-3 (→Took Out LC's of I14032, B0014, R0051)
- 11:05, 3 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-3 (→Took Out LC's of I14032, B0014, R0051)
- 10:38, 3 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-3 (New page: ===Took Out LC's of I14032, B0014, R0051=== *1ml for glycerol stock *rest miniprepped (for sequencing and 3A ligation) **when spun down, B0014 cells slightly reddish tint?)
- 09:23, 1 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-1 (New page: ==Agenda== *Decide on constitutive promoter for CPX **Transform promoter into DH5a *Check sequencing results for Mer/GFP 3A)
- 18:42, 31 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-31 (→CPX Immunostaining)
- 18:42, 31 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-31 (→CPX Immunostaining)
- 18:36, 31 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-31
- 18:32, 31 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-30
- 15:13, 27 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-27 (New page: ==Agenda== *dilute LC's of Mer/GFP Transformations and BL21 untransformed cells **Miniprep LC's of Mer/GFP *Finish Western Blot *Order EC20 oligos)
- 15:22, 25 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-25 (→Agenda)
- 11:29, 23 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-23 (→Agenda)
- 11:28, 23 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-23 (→Agenda)
- 11:28, 23 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-23
- 10:23, 23 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-23 (→Digestion of I13500)
- 10:19, 23 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-23 (→Digestion of I13500)
- 10:18, 23 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-23 (→Digestion of I13500)
- 10:17, 23 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-23 (→Agenda)
- 10:00, 23 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-23 (→Agenda)
- 16:28, 22 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-21 (→LC DH5a with I13500 from 7/20 Plate)
- 16:27, 22 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-21 (→LC DH5a from Plate(7/20))
- 16:16, 22 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-22
- 16:12, 22 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-22 (→Miniprep DH5a with I13500 from 7/20 Plate)
- 16:10, 22 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-22 (→Miniprep DH5a with I13500 from 7/20 Plate)
- 15:27, 22 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-23 (New page: ==Agenda== *Polystyrene Assay **Morning: get correct OD **Add AHL **Afternoon: Test *Digest I3500 *Ligate 3A with I3500 and Mer in pSB1AT3 (so that, if we combine with polystyrene-binding ...)
- 15:05, 22 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-22 (New page: ===Checked on DH5a LC's=== *all four grew *placed in 4C ===Miniprep DH5a with I13500 from 7/20 Plate===)
- 15:04, 22 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-21
- 15:03, 22 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-21 (New page: ===LC DH5a from Plate(7/21)=== *took 4 colonies ===LC DH5a with I13500 from 7/21 Plate=== * took 2 colonies (of 7 total))
- 09:37, 20 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-20 (New page: ==Agenda== *Dilute liquid cultures of Colony PCR 3A(F+B+CPX) #5,6,8 **Check antibiotic resistance screening plates first **Some for Miniprep ***Some of Miniprep for sequencing **Some for g...)
- 16:53, 19 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-19 (→Liquid Culture of BBa_I13500)
- 16:52, 19 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-19
- 12:21, 19 July 2007 (hist) (diff) IGEM:MIT/2007/Protocols (→DNA Work)
- 12:20, 19 July 2007 (hist) (diff) IGEM:MIT/2007/Protocols (→DNA Work)
- 12:16, 19 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-19 (→Colony PCR of Yesterday's 3A Transformation)
- 11:58, 19 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-19 (→Colony Count of Yesterday's Plates)
- 17:41, 18 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-19 (→Agenda for Thursday, 7/19)
- 17:40, 18 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-18 (top)
- 17:37, 18 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17 (→Changes to the OWW "Transforming Chemically Competent Cells" Protocol for TK Cells)
- 15:52, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17
- 11:11, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17 (→Digestion of (F2620 and B0034) for 3A)
- 10:21, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17 (→Digestion of CPX for 3A)
- 17:25, 16 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17 (→Agenda)
- 12:55, 16 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-13 (New page: ==Checked Plates of Transformed 3A XL-1== *LB+Kan Plate - all had significant growth *LB+Kan+Amp: **1: very small colonies **2: no growth **3: some bits of dead cells on a streak, otherwis...)
- 11:31, 16 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-17 (New page: =Agenda= *Test Standard Assembly of pSB3k3 with F2620, B0034, E1010 using AHL **LC grown overnight)
- 11:30, 16 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-16 (→Today's Agenda)
- 11:29, 16 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-16 (→Sequencing of LC#1&2 from 3A)
- 11:29, 16 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-16
- 10:04, 12 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11 (→DD part E1010) (top)
- 18:36, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11 (→Diagnostic Double Digestion of Today's Miniprep of pSB3k3+F2620+B0034)
- 18:36, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11 (→Results for Transformed XL-1 (pSB3K3 + F2620 + B0034) Miniprep)
- 18:35, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11
- 18:34, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (top)
- 12:24, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11
- 11:33, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (→Plating)
- 11:18, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (→DH5alpha strain)
- 11:18, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (→Reaction Mix)
- 11:17, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (→Reaction Mix)
- 11:15, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (→Reaction Mix)
- 10:41, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (→Nanodrop of Purified pSB3k3 from Mini-Prep of DB3.1 LQ)
- 09:55, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-6 (→Transformed and plated another set) (top)
- 17:04, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11 (→Agenda)
- 17:03, 10 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-11 (New page: ===Agenda=== 1.) Miniprep Backbone LC, DD eRFP (+, - controls), DD miniprep backbone (do we need to gel or pcr purify?), Standard assembly by running gel, purifying gel, ligation, transfor...)
- 16:59, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-10 (top)
- 15:54, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-10
- 15:47, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-8 (→Digestion of F2620, B0034, pSB3k3) (top)
- 10:18, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-10 (→Checked Transformation Plates)
- 10:17, 10 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-10 (New page: ===Checked Transformation Plates=== DH5a in Amp (100µl) - 14 colonies DH5a in Amp (300µl) - 60 DH5a in Kan (100µl) - 4 DH5a in Kan (300µl) - 10 XL-1 in Amp (20µl) - 720 colonies XL-1...)
- 09:24, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-8
- 09:11, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-8 (→Part(Plate #/ Pick#/ Nanodrop))
- 09:10, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-8 (→Part/Plate #/ Pick#/ Nanodrop)
- 09:06, 10 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9
- 16:39, 9 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (→Digestion of Purified pSB3k3 from this morning)
- 16:37, 9 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-9 (→Nanodrop of Purified pSB3k3 from Mini-Prep of DB3.1 LQ)
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