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- 10:31, 5 April 2013 (hist) (diff) Cfrench:DNAPurification1 (top)
- 05:01, 18 October 2011 (hist) (diff) Cfrench:primerlist (top)
- 04:47, 1 August 2011 (hist) (diff) Cfrench:DNAPurification1
- 05:18, 8 March 2010 (hist) (diff) Cfrench:BacTrans1 (top)
- 07:59, 28 October 2009 (hist) (diff) Cfrench:CaseStudy1 (top)
- 07:58, 28 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 12:25, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 12:23, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 12:17, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 12:16, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 12:13, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 12:09, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 12:07, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 11:41, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 09:40, 22 October 2009 (hist) (diff) Cfrench:CaseStudy1
- 09:37, 22 October 2009 (hist) (diff) N Cfrench:CaseStudy1 (New page: =Synthetic Biology Case Study 1: Development of an arsenic biosensor= Back to main page)
- 09:35, 22 October 2009 (hist) (diff) French Lab
- 09:33, 22 October 2009 (hist) (diff) Cfrench:ScienceFiction (top)
- 09:19, 14 October 2009 (hist) (diff) Cfrench:ScienceFiction
- 05:25, 14 October 2009 (hist) (diff) Cfrench:ScienceFiction
- 05:21, 14 October 2009 (hist) (diff) Cfrench:ScienceFiction
- 05:20, 14 October 2009 (hist) (diff) Cfrench:ScienceFiction
- 05:18, 14 October 2009 (hist) (diff) Cfrench:ScienceFiction
- 04:58, 14 October 2009 (hist) (diff) N Cfrench:ScienceFiction (New page: ==Portrayal of Scientists in Science Fiction== 14 October 2009 ===The Questions=== Dear ICMB people, with apologies to those not interested: I have been asked to take part in a panel d...)
- 04:56, 14 October 2009 (hist) (diff) French Lab
- 10:24, 5 October 2009 (hist) (diff) Cfrench:compcellprep1 (top)
- 13:02, 1 May 2009 (hist) (diff) Cfrench:primerlist
- 04:16, 30 April 2009 (hist) (diff) Cfrench:BacTrans1
- 11:27, 29 April 2009 (hist) (diff) Cfrench:KodPCR (top)
- 11:25, 29 April 2009 (hist) (diff) Cfrench:KodPCR
- 09:18, 5 March 2009 (hist) (diff) Cfrench:compcellprep1
- 09:43, 31 October 2008 (hist) (diff) Cfrench:IdentifyingBacteria (top)
- 09:34, 31 October 2008 (hist) (diff) Cfrench:IdentifyingBacteria
- 12:49, 29 September 2008 (hist) (diff) Cfrench:IdentifyingBacteria
- 12:48, 29 September 2008 (hist) (diff) Cfrench:IdentifyingBacteria
- 12:47, 29 September 2008 (hist) (diff) Cfrench:IdentifyingBacteria
- 12:42, 29 September 2008 (hist) (diff) N Cfrench:IdentifyingBacteria (New page: ==Identification of Bacteria== 1. Obtain a colony of your bacterium of interest on a plate. 2. Suspend the colony in sterile DI water (about 0.1 ml). 3. Use as template in a PCR reactio...)
- 12:31, 29 September 2008 (hist) (diff) French Lab
- 06:07, 1 September 2008 (hist) (diff) Cfrench:BacTrans1
- 11:09, 14 August 2008 (hist) (diff) Cfrench:anthrone
- 11:08, 14 August 2008 (hist) (diff) N Cfrench:anthrone (New page: ==Assaying polysaccharides with the anthrone assay== ''CK will add this protocol.'')
- 11:07, 14 August 2008 (hist) (diff) French Lab
- 10:23, 18 July 2008 (hist) (diff) N Cfrench:KodPCR (New page: =Cloning parts by PCR using Kod polymerase= 18 July 2008. Kod Hot Start is a proof-reading polymerase distributed by Novagen. It is claimed to be as accurate as Pfu and 4 to 5 times fast...)
- 10:11, 18 July 2008 (hist) (diff) French Lab
- 04:59, 18 July 2008 (hist) (diff) Cfrench:primerlist
- 04:34, 18 July 2008 (hist) (diff) Cfrench:primerlist
- 10:35, 12 July 2008 (hist) (diff) Cfrench:primerlist
- 10:32, 12 July 2008 (hist) (diff) Cfrench:primerlist
- 10:22, 12 July 2008 (hist) (diff) Cfrench:primerlist
- 10:15, 12 July 2008 (hist) (diff) Cfrench:primerlist
- 10:01, 12 July 2008 (hist) (diff) Cfrench:primerlist
- 09:49, 12 July 2008 (hist) (diff) Cfrench:primerlist
- 09:47, 23 May 2008 (hist) (diff) Cfrench:compcellprep1
- 13:34, 19 February 2008 (hist) (diff) Cfrench:MABEL (top)
- 13:32, 19 February 2008 (hist) (diff) N Cfrench:MABEL (New page: ==MABEL: Mutagenesis with blunt-end ligation== This is broadly similar to the Stratagene QuickChange protocol but simpler and easier. So far it seems to be working pretty reliably. 1. De...)
- 13:20, 19 February 2008 (hist) (diff) French Lab
- 13:19, 19 February 2008 (hist) (diff) Cfrench:BALTIC (top)
- 13:18, 19 February 2008 (hist) (diff) Cfrench:BALTIC
- 13:17, 19 February 2008 (hist) (diff) French Lab
- 05:59, 19 February 2008 (hist) (diff) Cfrench:BALTIC
- 05:57, 19 February 2008 (hist) (diff) Cfrench:BALTIC
- 05:56, 19 February 2008 (hist) (diff) Cfrench:BALTIC
- 05:46, 19 February 2008 (hist) (diff) N Cfrench:BALTIC (New page: BALTIC: BioBrick Assembly by Ligation of Triple-digested Components return to main page)
- 05:44, 19 February 2008 (hist) (diff) French Lab
- 07:04, 20 December 2007 (hist) (diff) N Cfrench:glycerol (New page: ==Making Glycerol Stocks== ===From a plate=== *Transfer 0.7 ml sterile growth medium into a 1.5 ml microcentrifuge tube or a cryovial. *Add a generous loopful of cell material from the ...) (top)
- 07:00, 20 December 2007 (hist) (diff) French Lab
- 07:41, 27 November 2007 (hist) (diff) Cfrench:BioBrickVectors1 (top)
- 10:59, 21 November 2007 (hist) (diff) Cfrench:BioBrickVectors1
- 11:50, 19 November 2007 (hist) (diff) Cfrench:BioBrickVectors1
- 11:32, 19 November 2007 (hist) (diff) Cfrench:BioBrickVectors1
- 11:12, 19 November 2007 (hist) (diff) Cfrench:BioBrickVectors1
- 10:58, 19 November 2007 (hist) (diff) Cfrench:BioBrickVectors1
- 10:51, 19 November 2007 (hist) (diff) N Cfrench:BioBrickVectors1 (New page: ==Making a Broad Host Range BioBrick Vector== As part of the iGEM2007 competition, the University of Edinburgh team sought to make a broad host range BioBrick vector that could be used to...)
- 10:47, 19 November 2007 (hist) (diff) French Lab
- 10:29, 29 October 2007 (hist) (diff) Cfrench:primerlist
- 10:18, 29 October 2007 (hist) (diff) Cfrench:primerlist
- 12:56, 19 October 2007 (hist) (diff) Cfrench:BacTrans1
- 12:55, 19 October 2007 (hist) (diff) Cfrench:BacTrans1
- 12:46, 19 October 2007 (hist) (diff) N Cfrench:BacTrans1 (New page: ==Transformation of ''Bacillus subtilis''== This protocol was developed by Lucy Montgomery based on published protocols (Anagnostopoulos and SPizizen, 1961, J. Bacteriol. '''81:'''741 and ...)
- 12:30, 19 October 2007 (hist) (diff) French Lab
- 12:29, 19 October 2007 (hist) (diff) French Lab
- 09:34, 5 October 2007 (hist) (diff) Cfrench:compcellprep1
- 06:47, 13 September 2007 (hist) (diff) Cfrench:primerlist
- 10:48, 12 September 2007 (hist) (diff) French Lab
- 10:55, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 10:48, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 10:37, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 10:31, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 10:18, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 07:09, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 07:02, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 06:53, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 06:43, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 06:31, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 06:22, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 05:53, 21 August 2007 (hist) (diff) Cfrench:primerlist
- 14:10, 16 August 2007 (hist) (diff) Cfrench:primerlist
- 13:50, 16 August 2007 (hist) (diff) Cfrench:primerlist
- 13:42, 16 August 2007 (hist) (diff) Cfrench:primerlist
- 05:32, 16 August 2007 (hist) (diff) Cfrench:maxipreps1 (top)
- 05:30, 16 August 2007 (hist) (diff) N Cfrench:maxipreps1 (New page: ==Plasmid DNA maxipreps from ''E. coli''== ===Plasmid DNA maxiprep protocol for ''E. coli''=== *1. Transfer 50 ml sterile LB aseptically into a 250 ml flask and add the appropriate antib...)
- 05:28, 16 August 2007 (hist) (diff) French Lab
- 06:59, 9 August 2007 (hist) (diff) Cfrench:primerlist
- 06:51, 9 August 2007 (hist) (diff) Cfrench:primerlist
- 06:29, 27 July 2007 (hist) (diff) Cfrench:primerlist
- 06:20, 27 July 2007 (hist) (diff) N Cfrench:ProteinAssay (New page: ==Bradford Protein Assay using the Pierce Reagent== 1. Label a series of cuvettes 0, 0.2, 0.4, 0.6, 0.8 mg/ml. To each add 1 ml Bradford Assay Reagent (stored in the refrigerator). Add 0,...) (top)
- 06:18, 27 July 2007 (hist) (diff) French Lab
- 10:48, 23 July 2007 (hist) (diff) Cfrench:primerlist
- 10:36, 23 July 2007 (hist) (diff) Cfrench:primerlist
- 10:23, 23 July 2007 (hist) (diff) Cfrench:primerlist
- 06:41, 23 July 2007 (hist) (diff) Cfrench:primerlist
- 06:37, 23 July 2007 (hist) (diff) Cfrench:primerlist
- 06:35, 23 July 2007 (hist) (diff) N Cfrench:primerlist (New page: ==Catalog of primers== ===Primers for iGEM2007 flavours/fragrances project=== '''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c '''Purpose''': forward primer for ''ech (...)
- 06:29, 23 July 2007 (hist) (diff) French Lab
- 10:48, 22 July 2007 (hist) (diff) Cfrench:bbprimerdesign (top)
- 10:46, 22 July 2007 (hist) (diff) Cfrench:bbprimerdesign
- 10:42, 22 July 2007 (hist) (diff) Cfrench:bbprimerdesign
- 09:57, 22 July 2007 (hist) (diff) Cfrench:bbprimerdesign
- 12:57, 8 July 2007 (hist) (diff) N Cfrench:bbcombining1 (New page: ==10. Combining Biobricks== The outline procedure is covered in the Registry Help Pages under 'Standard Assembly'. The following is a more detailed procedure relating specifically to my l...) (top)
- 12:22, 8 July 2007 (hist) (diff) Cfrench:addingrbs (top)
- 12:21, 8 July 2007 (hist) (diff) m Cfrench:addingrbs
- 12:19, 8 July 2007 (hist) (diff) N Cfrench:addingrbs (New page: ==Adding a synthetic RBS to a biobrick coding sequence=== Since the Registry requires coding sequences to be provided without ribosome binding sites, you will often need to add one. For t...)
- 12:14, 8 July 2007 (hist) (diff) N Cfrench:restriction1 (New page: ===9. ANALYTICAL RESTRICTION DIGESTS=== To test the size or concentration of DNA or to determine the presence of restriction sites. For a single enzyme digest, mix in a 1.5 ml microcentri...) (top)
- 12:10, 8 July 2007 (hist) (diff) m Cfrench:minipreps1 (top)
- 12:10, 8 July 2007 (hist) (diff) Cfrench:minipreps1
- 12:07, 8 July 2007 (hist) (diff) N Cfrench:PCRScreening (New page: ==7. Screening colonies by PCR== This is an alternative to plasmid DNA minipreps, described in section 8. For screening purposes, where there is no intention to clone the PCR product, Taq...) (top)
- 12:00, 8 July 2007 (hist) (diff) N Cfrench:compcellprep1 (New page: ==6. Preparing and using competent ''E. coli'' cells== Competent cells are prepared by the method of Chung ''et al'' (1989): Chung, C.T., Niemela, S.L., and Miller, R.H. 1989. One-step p...)
- 11:53, 8 July 2007 (hist) (diff) N Cfrench:bbcloning (New page: ==5. Cloning a PCR product into a Biobrick vector== This section assumes that your PCR product is a Biobrick or Edinbrick. If not, you will have to adjust the procedure according to the r...) (top)
- 11:46, 8 July 2007 (hist) (diff) m French Lab
- 11:46, 8 July 2007 (hist) (diff) N Cfrench:DNAPurification1 (New page: ==4. Purifying a PCR product from solution== If your PCR product looks pure enough to continue with (ie a single band of the expected size, with no extra unwanted DNA bands), you can puri...)
- 11:39, 8 July 2007 (hist) (diff) N Cfrench:AGE (New page: ==3. Analysing DNA by Agarose Gel Electrophoresis== ===Materials required=== '''20 x TAE (Tris-acetate-EDTA buffer). ''' *Dissolve 48.4 g Tris base and 3.72 g sodium-EDTA in about 450 ml...) (top)
- 11:30, 8 July 2007 (hist) (diff) N Cfrench:PfuPCR (New page: ==2. Cloning DNA for new biobricks using Pfu polymerase== Pfu is a proof-reading polymerase from Promega. They claim that it is the most accurate polymerase available for PCR, making far ...) (top)
- 11:22, 8 July 2007 (hist) (diff) Cfrench:bbprimerdesign
- 11:21, 8 July 2007 (hist) (diff) Cfrench:labtoppage (top)
- 11:20, 8 July 2007 (hist) (diff) N Cfrench:bbprimerdesign (New page: ==1. Designing Primers to Make a New Biobrick== This is well covered in the [Registry notes (link to be added here). More will be added here later, but what I mainly want to point out is ...)
- 10:58, 8 July 2007 (hist) (diff) French Lab
- 11:05, 12 January 2007 (hist) (diff) N Cfrench:gramstain (top)
- 11:03, 12 January 2007 (hist) (diff) Cfrench:labtoppage
- 06:38, 10 January 2007 (hist) (diff) Cfrench:toxiclist (top)
- 10:07, 23 December 2006 (hist) (diff) Cfrench:labtoppage
- 10:06, 23 December 2006 (hist) (diff) Cfrench:labtoppage
- 10:05, 23 December 2006 (hist) (diff) Cfrench:labtoppage
- 10:02, 23 December 2006 (hist) (diff) Cfrench:multidomhem (top)
- 10:01, 23 December 2006 (hist) (diff) Cfrench:multidomhem
- 09:53, 23 December 2006 (hist) (diff) Cfrench:hemanalysis1 (top)
- 09:43, 23 December 2006 (hist) (diff) Cfrench:hemanalysis1
- 09:22, 23 December 2006 (hist) (diff) Cfrench:hemtoppage (top)
- 05:59, 7 December 2006 (hist) (diff) N Cfrench:DNAextraction1 (top)
- 05:55, 7 December 2006 (hist) (diff) Cfrench:minipreps1
- 05:53, 7 December 2006 (hist) (diff) N Cfrench:minipreps1
- 05:48, 7 December 2006 (hist) (diff) Cfrench:labtoppage
- 11:01, 5 December 2006 (hist) (diff) N Cfrench:multidomhem
- 10:39, 5 December 2006 (hist) (diff) Cfrench:hemtoppage
- 10:38, 5 December 2006 (hist) (diff) Cfrench:hemanalysis1
- 10:37, 5 December 2006 (hist) (diff) Cfrench:hemanalysis1
- 12:58, 1 December 2006 (hist) (diff) Cfrench:hemanalysis1
- 12:18, 1 December 2006 (hist) (diff) N Cfrench:hemanalysis1
- 11:51, 1 December 2006 (hist) (diff) Cfrench:hemtoppage
- 11:49, 1 December 2006 (hist) (diff) User:Cfrench (top)
- 11:48, 1 December 2006 (hist) (diff) Crench:labtoppage
- 11:47, 1 December 2006 (hist) (diff) Cfrench:labtoppage
- 12:54, 30 November 2006 (hist) (diff) m Cfrench:hemlist1 (top)
- 12:51, 30 November 2006 (hist) (diff) Cfrench:hemlist1
- 12:28, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 12:27, 30 November 2006 (hist) (diff) N Cfrench:hemlist1
- 12:26, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 12:24, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 11:49, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 11:34, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 11:21, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 11:14, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 10:59, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 10:56, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 10:47, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 10:30, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 10:29, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 10:17, 30 November 2006 (hist) (diff) Cfrench:hemtoppage
- 10:12, 30 November 2006 (hist) (diff) N Cfrench:hemtoppage
- 09:56, 30 November 2006 (hist) (diff) Cfrench:labtoppage
- 09:53, 30 November 2006 (hist) (diff) N Cfrench:labtoppage
- 09:53, 30 November 2006 (hist) (diff) User:Cfrench
- 09:52, 30 November 2006 (hist) (diff) Cfrench:toxiclist
- 09:50, 30 November 2006 (hist) (diff) N Crench:labtoppage
- 09:49, 30 November 2006 (hist) (diff) User:Cfrench
- 06:19, 29 November 2006 (hist) (diff) Cfrench:toxiclist
- 06:13, 29 November 2006 (hist) (diff) Cfrench:toxiclist
- 06:05, 29 November 2006 (hist) (diff) Cfrench:toxiclist
- 06:01, 29 November 2006 (hist) (diff) Cfrench:toxiclist
- 05:59, 29 November 2006 (hist) (diff) N Cfrench:toxiclist
- 05:28, 29 November 2006 (hist) (diff) User:Cfrench
- 12:02, 28 November 2006 (hist) (diff) User:Cfrench
- 11:31, 28 November 2006 (hist) (diff) User:Cfrench
- 11:29, 28 November 2006 (hist) (diff) User:Cfrench
- 11:27, 28 November 2006 (hist) (diff) N User:Cfrench
(Latest | Earliest) View (newer 250) (older 250) (20 | 50 | 100 | 250 | 500)
