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• 22:24, 30 September 2009 (hist) (diff) 20.309:Presentations‎ (→Optical Microscopy: Biomechanics)
• 00:06, 1 September 2009 (hist) (diff) MIT BE TA-Training-2009‎ (→Day 2: Tuesday, September 1st) (top)
• 14:01, 19 November 2008 (hist) (diff) m WFYellow(F08) Research Proposal‎ (→Allen and Jess's 20.109 Module 3 Research Proposal Resource Page)
• 13:59, 19 November 2008 (hist) (diff) WFYellow(F08) Research Proposal‎ (→Allen and Jess's 20.109 Module 3 Research Proposal Resource Page)
• 17:25, 12 November 2008 (hist) (diff) User:Jessho‎ (top)
• 17:22, 12 November 2008 (hist) (diff) WFYellow(F08) Research Proposal‎
• 17:15, 12 November 2008 (hist) (diff) WFYellow(F08) Research Proposal‎
• 16:54, 12 November 2008 (hist) (diff) N WFYellow(F08) Research Proposal‎ (New page: Idea: Identify proteins associated with extracellular Hsp90 Links to papers)
• 16:12, 12 November 2008 (hist) (diff) User:Jessho‎
• 15:11, 12 November 2008 (hist) (diff) 20.109(F08): Mod 3 Day 3 Journal Club II‎
• 16:58, 31 October 2008 (hist) (diff) Talk:20.109(F08): Mod 2 Day 8 Microarray data analysis‎
• 16:57, 31 October 2008 (hist) (diff) Talk:20.109(F08): Mod 2 Day 8 Microarray data analysis‎
• 16:55, 31 October 2008 (hist) (diff) N Image:WF Yellow YJR097W microaarray - analyzed.xls‎ (Sorted microarray results for 20.109(F08) WF Yellow group) (top)
• 14:44, 31 October 2008 (hist) (diff) Talk:20.109(F08): Mod 2 Day 8 Microarray data analysis‎
• 09:54, 16 October 2008 (hist) (diff) 20.109(F08): Sign up for journal club‎ (→Journal Club for Module 3)
• 15:33, 8 October 2008 (hist) (diff) User:Jessho‎
• 14:00, 1 October 2008 (hist) (diff) Talk:20.109(F08): Mod 1 Day 8 FACS analysis‎
• 22:55, 30 September 2008 (hist) (diff) Talk:20.109(F08): Mod 1 Day 6 Restriction map and tissue culture‎
• 17:07, 24 September 2008 (hist) (diff) Talk:20.109(F08): Mod 1 Day 5 Examine candidate clones‎
• 09:26, 9 September 2008 (hist) (diff) User:Jessho‎
• 09:20, 9 September 2008 (hist) (diff) 20.109(F08):People‎
• 11:01, 17 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-17‎ (→Agenda)
• 10:31, 17 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-17‎ (New page: ==Agenda== #Transform R0051 into BL21 #Transform last night's ligations (F+B, I.B34.CPX.B14) into BL21 #Plate transformants)
• 10:30, 17 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16‎ (→Ligation Mixtures) (top)
• 19:54, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16‎
• 19:30, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16‎ (→DD I14032.B0034 Mixture)
• 12:24, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16‎
• 12:01, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14‎ (→DD Nanodrop Results) (top)
• 12:00, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14‎ (→Double Digestion)
• 11:46, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14‎
• 11:45, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-14‎ (→Ligations - Take 2)
• 11:41, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-11‎ (top)
• 11:01, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-12‎ (→LC'ed 6 colonies each from cPCR #6 & #8 S.A. ligation of F+B+CPX+B0014 into pSB1AC3 in B-R cells) (top)
• 11:00, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-6‎ (→Transformed Ligation of F+B+CPX) (top)
• 01:24, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-16‎ (→Agenda)
• 01:24, 16 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-16‎ (New page: ==Agenda== #Make electro/chemically competent cells #Transform and plate R0051 #Make more DD R0051 #Redo R0051.B0034 ligation #DD I14032.B0034 and put it into pSB1AC3 as a blank vector)
• 01:23, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-15‎ (→All the things transformed and/or plated today) (top)
• 01:23, 16 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-15‎ (→Agenda)
• 10:13, 15 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-15‎ (→Agenda)
• 17:33, 14 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-15‎
• 10:32, 13 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10‎ (→Western Blot) (top)
• 10:30, 13 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-13‎ (New page: ==Agenda== #Check sequencing results of constitutive promoters + CPX #Dilute last night's LCs (F2620.B0034.CPX.B0014) #Miniprep half of last night's LCs and send in for sequencing #Live ce...)
• 12:25, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-11‎ (→Agenda)
• 12:13, 11 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-11‎ (→Agenda)
• 12:13, 11 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-11‎ (New page: ==Agenda== #Double digestion: #*R0051.B0034, I14032.B0034, CPX.B0014 - for 3A assembly into pSB1AC3 #*F2620.B0034.CPX, B0014 - for standard assembly and to figure out extra bands in wester...)
• 18:28, 10 August 2007 (hist) (diff) N Image:WesternblotCPX.jpg‎ (Western Blot of DH5a induced into expressing CPX at varying levels) (top)
• 18:27, 10 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-10‎
• 18:24, 10 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-10‎ (New page: ==Agenda== #Run Western Blot (at Belcher Lab with Rana and Robbie) ==Western Blot== Lanes: <pre> 1 Invitrogen BenchMark Ladder 2 T 1E-5 3 T 1E-7 4 T 1E-8 5 T 0 6 U 1E-5 7 U 1...)
• 18:19, 10 August 2007 (hist) (diff) N Image:WesternblotCPX.JPG‎ (Our second western blot of F2620.B0034.CPX) (top)
• 18:16, 10 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-8‎ (New page: ==Agenda== #Transform overnight ligations #Plate transformants #Make more plates.) (top)
• 16:03, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-7‎ (→Agenda)
• 16:01, 7 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-7‎
• 15:53, 7 August 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-8-6‎ (New page: ==Agenda== #Look at digestions on gel #PCR Purify or Nucleotide Purify digestion results #Ligate constitutive promoters to RBS, CPX to B0014 ==Expected Part Lengths== <pre> Name ...)
• 17:24, 2 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-1‎ (→Sequencing Results) (top)
• 17:23, 2 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-1‎
• 17:11, 2 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-31‎ (→CPX Immunostaining) (top)
• 17:10, 2 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-1‎ (→Results)
• 17:08, 2 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-30‎ (→Results of CPX Assay) (top)
• 17:07, 2 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-30‎ (→Live Cell Immunoassay (Eric))
• 12:33, 1 August 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-8-1‎ (→Agenda)
• 09:33, 31 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-31‎ (→Agenda)
• 09:14, 31 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-31‎ (New page: ==Agenda== #Run mercury assay #Redo live immunostaining with altered protocol #Enter working parts into BioBricks Registry #Find constitutive promoter and perform 3A assembly with a RBS an...)
• 15:52, 26 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-26‎ (top)
• 15:50, 26 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-25‎
• 12:40, 26 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-26‎ (→Results of First Plating (yesterday's mer+gfp+1AT3 transformation))
• 12:09, 26 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-26‎ (→Agenda)
• 12:01, 26 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-25‎ (→Agenda)
• 11:59, 26 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-26‎ (New page: ==Agenda== #Coomassie staining of AHL-induced CPX cells #Transform the second mer-gfp-1AT3 ligation #Pick and LC last night's plated transformations (mer-gfp-1AT3))
• 10:18, 25 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-25‎ (→merR/Pmer+I13500 Transformation Results)
• 10:17, 25 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-25‎ (→Agenda)
• 10:17, 25 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-25‎
• 10:14, 25 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-24‎ (→Transformation of Mer/I13500 after overnight ligation (in TK's DH5alpha))
• 10:13, 25 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-24‎ (→Transformation of CPX (A+,Cm+), FhuA (K+) and pUC18 (A+) into Barry's BL21 cells (more protein!))
• 10:12, 25 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-25‎ (New page: ==Agenda== #Make LC of last night's I13500+MerR/Pmer transformation #Make LC of last night's CPX and FhuA transformations into BL21 (eventually will make glycerol stocks) #Run western blot...)
• 09:35, 19 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-19‎ (→Agenda for Thursday, 7/19)
• 16:16, 18 July 2007 (hist) (diff) IGEM:MIT/2007/Inventory‎ (top)
• 12:35, 18 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-18‎ (→Transformed tk's competent cells)
• 12:34, 18 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-18‎ (New page: ==Agenda== #Transform tk's competent cells with yesterday's 3A assembly results (F2620+B0034 and CPX insert into vector pSB1AC3) #Make LB+Cm and LB+Cm+Kan plates #Meeting with TK and Drew ...)
• 12:18, 18 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-14‎ (→Transformation Protocol) (top)
• 17:17, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17‎ (→Ligation of F2620+B0034 and CPX into pSB1AC3)
• 13:40, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17‎ (→Miniprep Results for 5mL LCs of PT040 made on 7/16)
• 13:37, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17‎
• 09:34, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17‎ (→Digestion of (F2620 and B0034) for 3A)
• 09:33, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-14‎ (→Late Friday (7/13) Afternoon Update)
• 09:32, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17‎ (→Digestion of (F2620 and B0034) for 3A)
• 09:09, 17 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-17‎ (→Agenda)
• 09:40, 13 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-12‎ (top)
• 09:14, 12 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎ (→Double Digestion for Standard Assembly with eRFP)
• 09:13, 12 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎ (→DD part E1010)
• 09:13, 12 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎ (→Diagnostic Double Digestion of Today's Miniprep of pSB3k3+F2620+B0034)
• 09:12, 12 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-12‎ (New page: ==Agenda== #Run Gel #PCR Purify parts #Finish standard assembly of vector + E1010 insert)
• 17:10, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎ (→Notes on Future Use of Transformed XL-1)
• 17:08, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎
• 16:41, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎
• 15:44, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎
• 15:37, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎ (→Made More 1% Agarose in TAE Buffer)
• 15:31, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎
• 15:23, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-11‎ (→Diluted LC's of Transformed XL-1)
• 15:16, 11 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-11‎ (→made 1 liter 1% agarose solution) (top)
• 09:06, 9 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-8‎ (→Part/Plate #/ Pick#/ Nanodrop)
• 17:00, 7 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-7‎ (→Checked Plates 5 PM)
• 17:00, 7 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-7‎
• 16:59, 7 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-7‎
• 16:42, 7 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-7‎ (→Inoculating)
• 12:15, 7 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-7‎ (→Inoculating)
• 12:14, 7 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-7‎ (→Inoculating)
• 12:00, 7 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-7‎
• 09:27, 7 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-7‎ (New page: ==Agenda== #Pick plates and make liquid cultures ==Checked plates== <pre> B0034 (RBS) plated on 7/5/07 Plate # Colonies Notes 1 19 Overgrown, satellite colonie...)
• 09:09, 7 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-6‎
• 10:02, 5 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-5‎ (→Preparing Chemically Competent Cells)
• 09:59, 5 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-5‎ (→Preparing Chemically Competent Cells)
• 09:58, 5 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-5‎ (→Preparing Chemically Competent Cells)
• 09:52, 5 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-5‎ (→Making long term glycerol stock of DH5a)
• 09:51, 5 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-5‎ (→Preparing Chemically Competent Cells)
• 09:50, 5 July 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-7-5‎ (New page: ==Agenda== #Prepare chemically competent cells from the DH5a line #Make long term glycerol stock of DH5a ==DH5a Incubation== *both 10 mL overnight cultures had growth ==Making long term ...)
• 09:46, 5 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-3‎
• 09:45, 5 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-4‎ (top)
• 09:36, 5 July 2007 (hist) (diff) m IGEM:MIT/2007/Notebook/2007-7-3‎
• 09:35, 5 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-7-3‎
• 09:15, 4 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-29‎ (→Agenda for Friday, June 29) (top)
• 15:21, 3 July 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-14‎ (→Transformation Protocol)
• 17:03, 26 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-26‎ (top)
• 12:38, 26 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-25‎ (→OmpC with polystyrene (18) binding peptide as passenger peptide)) (top)
• 12:35, 26 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-25‎ (→CPX with polystyrene (18) binding peptide as passenger peptide))
• 12:31, 26 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-25‎ (→Passenger Peptide Oligos w/ Restriction Cuts Built In)
• 12:28, 26 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-25‎ (→OmpC with polystyrene (18) binding peptide as passenger peptide))
• 12:27, 26 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-25‎ (→CPX with polystyrene (18) binding peptide as passenger peptide))
• 12:25, 26 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-25‎ (→USING SEQUENCHER (Demo))
• 09:32, 25 June 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-6-24‎ (New page: ==Meeting notes== ===Stuff we found after researching=== '''Semmie:''' Atheriosclerosis, what it is. Lipase production in E. coli (gene LipA). Probiotics-lactobacillus. Major obstacle:...) (top)
• 19:32, 22 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-22‎ (top)
• 19:26, 22 June 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-6-22‎ (New page: ==Undergrad meeting notes== *will you guys add the first half here? Decided on Focus: bioremediation with Semmie and Bernice spending another day to research arterial plaque-targeting an...)
• 15:21, 20 June 2007 (hist) (diff) N IGEM:MIT/2007/Notebook/2007-6-20‎ (New page: ==To-Do== #Research: find a peptide sequence small enough to express as the passenger protein in eCPX #Grad meeting at 3 *Grad meeting canceled ==Interpretation of CPX Paper== So, we've ...) (top)
• 11:08, 19 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-19‎ (→Rough Layout of surface sticky protein) (top)
• 10:58, 19 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-18‎ (top)
• 10:20, 19 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-19‎
• 10:19, 19 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-19‎ (→Rough Layout of surface sticky protein)
• 10:14, 19 June 2007 (hist) (diff) N Image:Stickysurfaceexpression.jpg‎ (Rough sketch of the expression of sticky mussel foot protein on the surface of E. Coli via CPX. <biblio>Rice07 pmid=16600968</biblio>) (top)
• 10:09, 19 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-19‎
• 12:30, 15 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-15‎ (→Results from last night's incubation)
• 11:55, 15 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-15‎ (→Plan)
• 11:50, 15 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-15‎ (→Results from last night's incubation)
• 11:48, 15 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-15‎ (→Results from last night's incubation)
• 11:46, 15 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-14‎ (→Procedures)
• 11:35, 15 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-15‎ (→LAB WORK)
• 11:26, 15 June 2007 (hist) (diff) IGEM:MIT/2007/Notebook/2007-6-12‎
• 23:30, 10 June 2007 (hist) (diff) IGEM:MIT/2007/Contacts‎ (→Team Contact Info)
• 00:17, 10 June 2007 (hist) (diff) IGEM:MIT/2007/Ideas‎ (→Random ideas from Superphage (Forrest))

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