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• 21:04, 16 September 2008 (hist) (diff) N IGEM:PennState/Labbook/GarrettTobin08/2008/09‎ (Autocreate Month 2008/09 Entry for IGEM:PennState/Labbook/GarrettTobin08) (top)
• 21:04, 16 September 2008 (hist) (diff) N IGEM:PennState/Labbook/GarrettTobin08/2008/09/17‎ (Autocreate 2008/09/17 Entry for IGEM:PennState/Labbook/GarrettTobin08) (top)
• 15:51, 15 April 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-4-15‎ (New page: <h1>April 4, 2007</h1> *Replated all fridge plates *Made plates (60 amp) 2-) (top)
• 15:50, 15 April 2008 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson‎ (top)
• 19:20, 26 February 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-2-26‎ (New page: <h1>Feb. 26, 2007</h1> *electrocomp. cell prep 4:45-) (top)
• 21:23, 22 February 2008 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2008-2-21‎ (top)
• 21:23, 22 February 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-2-22‎ (New page: <h1>Feb. 22, 2008</h1> *Finished replating 7:30-8:30) (top)
• 18:16, 21 February 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-2-21‎ (New page: <h1>Feb. 21, 2008</h1> *Made plates **20 LB amp **20 LB kan *Replated all fridge plates *Autoclaved tips and contaminated media 3:30-)
• 15:48, 27 January 2008 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2008-1-27‎ (Removing all content from page) (top)
• 15:44, 27 January 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-1-27‎ (New page: <h1>January 27, 2008</h1> 2:30-)
• 15:01, 11 January 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-1-6‎ (New page: <h1>January 6, 2007</h1> *Worked on presentation *took 48hr timepoint 12-2, 9-11) (top)
• 14:54, 11 January 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-12-27‎ (New page: <h1>December 27, 2007</h1> 12-7) (top)
• 13:48, 6 January 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-1-5‎ (New page: <h1>January 6, 2007</h1> *Took 24h data point *Ran samples in IC overnight 1-3) (top)
• 13:46, 6 January 2008 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2008-1-4‎ (top)
• 17:09, 4 January 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-1-4‎ (New page: <h1>January 4, 2008</h1> *fermentation test run 11-)
• 17:08, 4 January 2008 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2008-1-3‎ (top)
• 16:11, 3 January 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-1-3‎ (New page: <h1>January 3, 2007</h1> *Innoculated W3110 for fermentation run tomorrow 10-)
• 16:10, 3 January 2008 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2008-1-2‎ (New page: <h1>January 2, 2007</h1> *Made LB Amp plates *Replated 1-6) (top)
• 14:46, 30 December 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-12-30‎ (top)
• 12:49, 30 December 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-12-30‎ (New page: <h1>December 30, 2007</h1>)
• 13:43, 29 December 2007 (hist) (diff) Nm IGEM:PennState/Labbook/NoahJohnson/2007-12-29‎ (New page: <h1>December 29, 2007</h1> *Replated all plates in fridge *Reinnoculated for test run tomorrow 10-1) (top)
• 18:03, 28 December 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-12-28‎ (New page: <h1>December 28, 2007</h1> *Made M9 min media *Prepped for test fermentation run tomorrow 1-5) (top)
• 11:39, 21 December 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson‎
• 11:39, 21 December 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson‎
• 11:38, 21 December 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson‎
• 17:07, 20 November 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-11-20‎ (New page: <h1> Nov. 20, 2007 </h1> *Submitted crp* for sequencing *made LB Amp, and LB Kan plates *replated fridge plates 12-5) (top)
• 01:49, 31 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-30‎ (New page: <h1>October 30, 2007</h1> *Worked on poster 4-9) (top)
• 01:48, 31 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-29‎ (New page: <h1> October 29, 2007</h1> *Group Meeting for practice presentation 4:30-5:30) (top)
• 18:02, 30 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-27‎ (New page: <h1> October 27, 2007</h1> *Worked on presentation 4-12) (top)
• 22:44, 25 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-25‎ (New page: <h1>October 25, 2007</h1> *worked on website 5:30-10:30) (top)
• 19:43, 25 October 2007 (hist) (diff) IGEM:PennState/2007/Lab-photos‎ (top)
• 19:42, 25 October 2007 (hist) (diff) IGEM:PennState/2007/Lab-photos‎
• 19:41, 25 October 2007 (hist) (diff) IGEM:PennState/2007/Lab-photos‎
• 11:09, 25 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-22‎ (New page: <h1>October 25, 2007</h1> *Group meeting @ 4pm *Replated fridge plates 3:30-6:30) (top)
• 22:29, 24 October 2007 (hist) (diff) User:Nrj5011‎ (top)
• 17:33, 18 October 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-10-16‎ (top)
• 17:33, 18 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-18‎ (New page: <h1>October 18, 2007</h1> *Picked up primers (1457 & 1458) **1457: pSB1A2 Forward **1458: pSB1A2 Reverse *Sent in crp* for sequencing 4-) (top)
• 17:16, 16 October 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-10-16‎
• 12:22, 16 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-16‎ (New page: <h1>October 16, 2007</h1> *Filled pipet tip boxes *Miniprepped crp* in psB1A2 *Replated crp* from overnight culture on two LB Amp plates 12-)
• 18:07, 15 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-15‎ (New page: <h1>October 15, 2007</h1> *Innoculated from transformation plate *Re-plated working transformation *Filled pipet-tip boxes *Group meeting @5 3:30-5:30) (top)
• 21:22, 14 October 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-10-14‎ (top)
• 13:09, 14 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-14‎ (New page: <h1>October 14, 2007</h1> *Made LB Amp plates (40) *Electrocomp. cell prep 12-)
• 19:24, 11 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-11‎ (New page: <h1>October 11, 2007</h1> *Ran restriction products on a gel *Gel extracted crp* and psB1A2 *crp* & psB1A2 overnight ligation 4:30-8) (top)
• 16:43, 11 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-10‎ (New page: <h1>October 10, 2007</h1> *E+S overnight restriction digest on pSB1A2 and crp* 9-10) (top)
• 09:18, 10 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-9‎ (New page: <h1>October 10, 2007</h1> *Electroporation transformation in Dr. Cirino's lab 4-10) (top)
• 00:45, 9 October 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-10-8‎ (top)
• 19:28, 8 October 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-10-8‎
• 19:02, 8 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-7‎ (New page: <h1>October 7, 2007</h1> *Transformed via electroporation in Dr.Cirino's lab 7-10) (top)
• 19:00, 8 October 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-10-8‎ (New page: <h1>October 8, 2007</h1> *Made 20 tubes of Amp stock solution **100mg/mL, 1mL in each tube *group meeting @ 5:30 4:30-7,)
• 19:39, 30 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-24‎ (New page: <h1>September 24, 2007</h1> *Cleaned up in lab *group meeting @ 4:30 3:30-5:30) (top)
• 23:40, 20 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-20‎ (top)
• 22:11, 20 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-20‎
• 18:53, 20 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-20‎ (New page: <h1>September 20, 2007</h1> *DH5a competent cell prep 6-)
• 18:52, 20 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-19‎ (top)
• 16:00, 19 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-19‎ (New page: <h1>September 19, 2007</h1> *DH5a competent cell prep 3:30-)
• 18:30, 18 September 2007 (hist) (diff) IGEM:PennState/2007/Lab-photos‎
• 18:16, 18 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-18‎ (top)
• 18:12, 18 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-18‎ (New page: <h1>September 18, 2007</h1> *Innoculated DH5a starter cultures off of plate from Cirino's lab. *Prepared for competent cell prep tomorrow. *Innoculated new DH5a plates and left in incuba...)
• 10:59, 17 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-17‎ (top)
• 09:46, 17 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-17‎ (New page: <h1>September 17, 2007</h1> *Competent cell prep 9:30-)
• 19:58, 16 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-16‎ (top)
• 18:50, 16 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-16‎ (New page: <h1>September 16, 2007</h1> 6:30-)
• 22:58, 11 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-11‎ (top)
• 17:55, 11 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-11‎ (New page: <h1>September 11, 2007</h1> *Restriction digest (E+S) on P1010 in psB1A2 *Starting cuture of E0240 in psB1A2 to miniprep and restrict tommorrow if P1010 doesn't fall out 5:30-)
• 17:53, 11 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-10‎ (New page: <h1>September 10, 2007</h1> *Miniprepped crp* and P1010 in psB1A2 *Group meeting @ 4:30 3:30-5) (top)
• 16:15, 9 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-9‎ (New page: <h1>September 9, 2007</h1> *Innoculated crp* and P1010 in psB1A2 cultures *Tentative Plan: **Miniprep psB1A2 tommorrow **Restrict and extract **Quantify DNA to ensure I am not overloading ...) (top)
• 17:12, 7 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-7‎ (top)
• 16:45, 7 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-7‎
• 16:44, 7 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-7‎
• 11:01, 7 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-7‎ (New page: <h1>September 7, 2007</h1> *Weekend plan: **Friday evening: Transform into DH5a, plate on LB Amp, Also innoculate culture of DH5a **Saturday: Innoculate cultures from plates if transformat...)
• 18:51, 6 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-6‎ (top)
• 18:32, 6 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-5‎ (top)
• 18:31, 6 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-5‎
• 18:31, 6 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-6‎ (New page: <h1>September 6, 2007</h1> *Ligated crp* and psB1A2. **Used a 6:2 vect:ins ratio. **Ligated overnight in 16C fridge 6-)
• 19:32, 5 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-5‎ (New page: <h1>September 5, 2007</h1> *Made more 1X TAE buffer **Added 40mL of 50X TAE buffer to 4 L flask **Added 1960mL dH20 and mixed *Restriction digest on crp* and P1010 in psB1A2 **cut with Eco...)
• 19:30, 5 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-3‎ (top)
• 17:32, 3 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-3‎
• 17:32, 3 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-3‎ (New page: <h1>September 3, 2007</h1> *Ran gel on PCR products *PCR purified the 2 PCR rxns which worked: **0.01uL crp* template Pfu Polymerase **1.0uL crp* template Phusion Polymerase *Restricted th...)
• 17:29, 3 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-2‎ (top)
• 14:40, 2 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-2‎ (New page: <h1>September 2, 2007</h1> *Made sybr green *made gel 1:30-3)
• 14:40, 2 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-9-1‎ (top)
• 20:36, 1 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-9-1‎ (New page: <h1>September 1, 2007</h1> *ran touchdown PCR with Taq, Pfu, and Phusion on crp*)
• 20:35, 1 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-27‎ (New page: <h1>August 27, 2007</h1> Group meeting @5 5-6) (top)
• 20:30, 1 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-22‎ (top)
• 20:29, 1 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-20‎ (top)
• 20:29, 1 September 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-21‎ (top)
• 20:28, 1 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-24‎ (New page: <h1>August 24, 2007</h1> *Blasted sequencing results 9-3) (top)
• 20:26, 1 September 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-23‎ (New page: <h1>August 23, 2007</h1> Out of town) (top)
• 16:39, 22 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-22‎
• 12:30, 22 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-22‎ (New page: <h1>August 22, 2007</h1> *Made DH5a competent cells to transform into 11:30-)
• 12:30, 22 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-21‎
• 15:12, 21 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-21‎
• 13:39, 21 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-21‎ (New page: <h1>August 21, 2007</h1> 9:30-)
• 17:25, 20 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-20‎
• 11:37, 20 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-20‎ (New page: <h1>August 20, 2007</h1> PCR purified the working crp* rxn from friday (0.1uL template crp* DNA with Phusion polymerase) 10-)
• 11:36, 20 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-17‎ (top)
• 16:12, 17 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-17‎ (New page: <h1>August 17, 2007</h1> *Chromosomal Prep on crp* and W3110 *Ran PCR with Phusion and Taq on crp* **used 1, 0.1, 0.01, and 0.001uL crp* template DNA in different rxns *Ran gel on PCR pro...)
• 16:11, 17 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-16‎ (top)
• 12:48, 17 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-14‎ (top)
• 12:47, 17 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-16‎ (New page: <h1>August 16, 2007</h1> 9:30-4)
• 12:46, 17 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-15‎ (top)
• 12:45, 17 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-13‎ (top)
• 12:44, 17 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-10‎ (top)
• 16:07, 15 August 2007 (hist) (diff) Nm IGEM:PennState/Labbook/NoahJohnson/2007-8-15‎ (New page: <h1>August 15, 2007</h1> *Miniprepped crp* and B0034 *Ran restriction digest for suffix insertion of crp* after B0034 **Cut crp* with Xba and Pst **Cut B0034 with Spe and Pst *Ran on gel ...)
• 16:05, 15 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-14‎ (New page: <h1>August 14, 2007</h1> *Made competent cells *Innoculated plates and cultures with crp* and B0034 *Made 60 LB Amp plates 10-3)
• 17:12, 13 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-11‎ (top)
• 17:12, 13 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-13‎ (New page: <h1>August 13, 2007</h1> *Two transformations seemed to be successful: **3uL 6uL ins: 2uL vect **5uL 6uL ins: 2uL vect **Both non-heat-inactivated *innoculated 4 colonies from each plat...)
• 15:17, 12 August 2007 (hist) (diff) N IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-12‎ (New page: '''August 12''' *Transformed ligations **2-5) (top)
• 15:16, 12 August 2007 (hist) (diff) N IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-11‎ (New page: '''August 11th''' *Retried restricton **gel looked very good *Extracted *Started overnignt ligation at 16 C **1-5) (top)
• 15:15, 12 August 2007 (hist) (diff) IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-10‎ (top)
• 16:21, 11 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-11‎ (New page: <h1>August 11, 2007</h1> *Transformed ligations into DH5a and plated 2-)
• 16:22, 10 August 2007 (hist) (diff) N IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-10‎ (New page: '''August 10th''' *Minipreped Innoculations *Restricted **xylR cut with X and P **B0034 cut with S and P **12:30)
• 16:21, 10 August 2007 (hist) (diff) N IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-9‎ (New page: '''August 9th''' *Transformed Ligations **Nothing grew *Inooculated **xylR ORF **B0034 **Puc 19 * **12:30-4:30) (top)
• 16:19, 10 August 2007 (hist) (diff) IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-8‎ (top)
• 13:48, 10 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-10‎
• 13:45, 10 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-10‎ (New page: <h1>August 10, 2007</h1> 10-)
• 13:44, 10 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-9‎ (top)
• 10:03, 9 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-9‎ (New page: <h1>August 9, 2007</h1> 9:30-)
• 19:13, 8 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-8‎ (top)
• 11:07, 8 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-8‎
• 11:07, 8 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-8‎ (New page: <h1>August 8, 2007</h1> *Ran gel on last night's PCR products *Made TB solution for making DH5a chem-competent cells today)
• 11:06, 8 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-7‎ (top)
• 11:06, 8 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-7‎
• 13:15, 7 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-7‎
• 10:03, 7 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-6‎ (top)
• 10:02, 7 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-7‎ (New page: <h1>August 7, 2007</h1> 9:30-)
• 13:15, 6 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-3‎ (New page: <h1>August 3, 2007</h1> *Out of town for the day) (top)
• 11:11, 6 August 2007 (hist) (diff) Nm IGEM:PennState/Labbook/NoahJohnson/2007-8-6‎ (New page: <h1>August 6, 2007</h1> *Made LB media plates: **40 LB Kan **20 LB Amp+Kan 9:30-)
• 19:44, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎ (top)
• 19:42, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-26‎ (top)
• 19:41, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-27‎ (top)
• 18:43, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:42, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:42, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:40, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:35, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:31, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:29, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:20, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:19, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:15, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:03, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:03, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 18:03, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 17:48, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 17:48, 2 August 2007 (hist) (diff) IGEM:PennState/2007‎
• 17:00, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 17:00, 2 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎
• 11:37, 2 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-2‎ (New page: <h1>August 2, 2007</h1> *Ordered UV cuvettes *Began growing up 6 3ml cultures of crp* in LB *Will TELT dna prep them tonight or tomorrow)
• 19:43, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎ (top)
• 19:07, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎
• 18:58, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎
• 18:53, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎
• 15:20, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎
• 11:05, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎
• 11:05, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-31‎ (top)
• 11:04, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-31‎
• 10:51, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-31‎
• 10:49, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎
• 10:48, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎
• 10:48, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎
• 10:31, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/GalenLynch/2007-7-31‎ (top)
• 10:30, 1 August 2007 (hist) (diff) N IGEM:PennState/Labbook/GalenLynch/2007-8-1‎ (New page: <h1>August 1st, 2007: 10:15-?:??</h1>)
• 10:10, 1 August 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-8-1‎ (New page: <h1>August 1, 2007</h1> *Ran gel on PCR products 9:30)
• 10:09, 1 August 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-31‎
• 10:13, 31 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-30‎ (top)
• 10:12, 31 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-31‎ (New page: <h1>July 31, 2007</h1> 9:30-)
• 15:34, 30 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-28‎ (top)
• 15:34, 30 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-28‎ (New page: <h1>July 28, 2007</h1> Checked transformation plates Transformation seemed to be unsuccessful)
• 15:32, 30 July 2007 (hist) (diff) IGEM:PennState/2007‎
• 13:03, 30 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-30‎ (New page: <h1>July 30, 2007</h1> *Re-streaked old plates from 4C fridge 9:30-)
• 13:00, 30 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-27‎
• 11:43, 27 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-27‎
• 11:42, 27 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-27‎
• 11:30, 27 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-27‎ (New page: <h1>July 27, 2007</h1> Motility Results: *Pictures taken at 13 hrs (9:45am) *All plates 0.28% Eiken agar Plate 1: IPTG+ amp Swarmed: 2,4,5,12,13,14 Didn't Swarm: 6,8,9,10,11,15 Plate 2...)
• 21:05, 26 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-26‎
• 21:02, 26 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-26‎
• 20:47, 26 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-26‎
• 20:26, 26 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-26‎
• 20:23, 26 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-26‎
• 12:20, 26 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-26‎
• 09:39, 26 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎ (top)
• 09:37, 26 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-26‎ (New page: <h1>July 26, 2007</h1> 9:30-)
• 15:23, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 14:02, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 12:12, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 12:11, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 12:11, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 11:03, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 10:52, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 10:51, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 10:50, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎
• 10:19, 25 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-24‎ (top)
• 10:18, 25 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-25‎ (New page: <h1>July 25, 2007</h1> *DNA Precipitation 9:30-)
• 14:54, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-24‎
• 14:52, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-24‎
• 14:49, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-24‎
• 14:45, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎ (top)
• 14:44, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-24‎
• 10:21, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 10:21, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 10:20, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 10:20, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 10:19, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 10:19, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 10:18, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 10:16, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 09:34, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 09:33, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 09:33, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 09:30, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 09:29, 24 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎
• 09:21, 24 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-24‎ (New page: <h1>July 24, 2007</h1> *Poured Gel 9-)
• 13:34, 23 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-23‎ (New page: <h1>July 24, 2007</h1> *5uL 10X Pfx Amplification buffer/ ThermoPol)
• 21:04, 21 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-19‎ (top)
• 21:02, 21 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-20‎ (New page: <h1>July 20, 2007</h1> *Gel Extraction *Made BSA and enzyme aliquotted stock solutions 9:30-3:30) (top)
• 21:01, 21 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-21‎ (New page: <h1>July 21, 2007</h1> *Innoculated tubes *Left in 37C shaker 12-1) (top)
• 20:59, 21 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-18‎ (top)
• 20:56, 21 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-11‎ (top)
• 14:12, 19 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-12‎ (New page: <h1>July 19, 2007</h1> *Picked up stuff from stockroom and knock-out strains from Cirino Lab *Made LB plates (24 kept in -4C fridge) *Made SOB 9-6:30) (top)
• 14:11, 19 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-13‎ (top)
• 14:11, 19 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-19‎
• 15:02, 18 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-18‎ (New page: <h1>July 18, 2007</h1> *Submitted parts for sequencing *Began labels for glycerol stocks 9:30-2)
• 00:53, 18 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-17‎ (top)
• 17:17, 17 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-17‎
• 17:16, 17 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-17‎
• 11:11, 17 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-17‎ (New page: <h1>July 17, 2007</h1>)
• 11:10, 17 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-16‎ (New page: <h1>July 16, 2007</h1> *Innoculated off yesterdays plates into 2mL LB **LB Amp 1741005+E0240 in psB1A2 (4uL) 7/14 (8 colonies) **LB Amp 1741005+E0240 in psB1A2 (8uL) 7/14 (5 colonies) **...) (top)
• 18:49, 12 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-13‎ (New page: <h1>July 12, 2007</h1> *Picked up stuff from stockroom and knock-out strains from Cirino Lab *Made LB plates (24 kept in -4C fridge) *Made SOB 9-6:30)
• 08:35, 12 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-11‎
• 13:42, 11 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-10‎ (New page: <h1>July 10, 2007</h1>) (top)
• 13:41, 11 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-11‎ (New page: <h1>July 11, 2007</h1> *Filled pipet tip boxes *Gel Extraction with restricted vector and insert: **PSB1A2 vector **E0240 *Put in Galen's -20C box)
• 14:54, 9 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-9‎ (New page: <h1>July 9, 2007</h1> *Filled pipet tip boxes *Transformation *Made more SOB tubes, 500uL in each for easy transformations) (top)
• 15:27, 6 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-6‎ (top)
• 14:46, 6 July 2007 (hist) (diff) IGEM:PennState/Labbook/GalenLynch/2007-7-6‎
• 14:44, 6 July 2007 (hist) (diff) IGEM:PennState/Labbook/GalenLynch/2007-7-5‎ (top)
• 14:13, 6 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-6‎
• 10:32, 6 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-6‎ (New page: <h1>July 6, 2007</h1> *Yesterdays motility plates found that motility was greatest at 26°C so today we are running 5 motility trials at 26°C with varying concentrations of eiken agar *Ma...)
• 12:50, 5 July 2007 (hist) (diff) IGEM:PennState/2007/FreezeandSqueeze‎ (top)
• 12:47, 5 July 2007 (hist) (diff) N IGEM:PennState/2007/FreezeandSqueeze‎ (New page: '''Freeze 'N Squeeze DNA Gel Extraction''' 1. Electrophorese the DNA sample in an ararose gel (TAE or TBE), then stain with an appropriate reagent, e.g., ethidium bromide or SYBR Green 1....)
• 12:38, 5 July 2007 (hist) (diff) IGEM:PennState/2007‎
• 12:37, 5 July 2007 (hist) (diff) IGEM:PennState/2007‎
• 12:25, 5 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-5‎
• 11:41, 5 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-5‎
• 10:57, 5 July 2007 (hist) (diff) IGEM:PennState/2006/Eikenplates‎
• 10:53, 5 July 2007 (hist) (diff) IGEM:PennState/Labbook/LucienWeiss‎
• 10:21, 5 July 2007 (hist) (diff) IGEM:PennState/Labbook/LucienWeiss‎
• 10:03, 5 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-4‎ (top)
• 10:03, 5 July 2007 (hist) (diff) IGEM:PennState/Labbook/NoahJohnson/2007-7-5‎
• 10:01, 5 July 2007 (hist) (diff) N IGEM:PennState/Labbook/NoahJohnson/2007-7-5‎ (New page: <h1>July 5, 2007</h1> *Ran Ligation (Started 9:45))

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