User contributions
From OpenWetWare
(Latest | Earliest) View (newer 250) (older 250) (20 | 50 | 100 | 250 | 500)
- 14:04, 5 March 2013 (hist) (diff) Sean Lauber:MTT Cell Proliferation Assay (top)
- 17:35, 25 January 2013 (hist) (diff) N Sean Lauber:Thawing Mammalian Cells (New page: 1. Remove a vial from liquid nitrogen and transport (quickly) on ice (best is dry ice but ice is okay) 2. Thaw the vial in a 37°C water bath by constantly swirling the vial in the bath (...) (top)
- 17:27, 25 January 2013 (hist) (diff) Sean Lauber:Culturing Lewis Lung Carcinoma Cells (top)
- 17:27, 25 January 2013 (hist) (diff) Sean Lauber:Culturing Lewis Lung Carcinoma Cells
- 11:59, 25 January 2013 (hist) (diff) Sean Lauber:Adenovirus infection (in vitro) (top)
- 11:59, 25 January 2013 (hist) (diff) Sean Lauber:Adenovirus infection (in vitro)
- 14:28, 11 December 2012 (hist) (diff) User:Sean E. Lauber (top)
- 13:16, 11 December 2012 (hist) (diff) N Sean Lauber:Culturing of Primary Macrophages (New page: This procedure describes how to culture macrophages derived from from the broncho-alveolar lavage. However, this can be applied to any source of cells where you'd like to isolate an adhere...) (top)
- 12:57, 11 December 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 12:57, 11 December 2012 (hist) (diff) Sean Lauber:Culturing Lewis Lung Carcinoma Cells
- 12:43, 11 December 2012 (hist) (diff) N Sean Lauber:Culturing Lewis Lung Carcinoma Cells (New page: ==Thawing the culture== 1. Thaw a vial of 3 million cells/ml in a 37°C water bath 2. Add 1 ml of PBS and add this 2 ml to 8 ml of PBS 3. Spin at 1000 rpm for 3 min to pellet 4. Discar...)
- 12:14, 11 December 2012 (hist) (diff) Sean Lauber:Adenovirus infection (in vitro)
- 15:50, 10 December 2012 (hist) (diff) N Sean Lauber:Adenovirus infection (in vitro) (New page: This procedure is optimized for using 96-well plates (0.32 cm^2). 1. Seed 2000 cells per well 2. Incubate overnight at 37°C/5% CO2. 3. Wash cells with 100 ul of PBS two times. 4. Add ...)
- 15:13, 10 December 2012 (hist) (diff) User:Sean E. Lauber (→Mammalian Cell Culture)
- 14:03, 10 December 2012 (hist) (diff) Sean Lauber:MTT Cell Proliferation Assay
- 13:13, 10 December 2012 (hist) (diff) N Sean Lauber:MTT Cell Proliferation Assay (New page: The following protocol has been optimized for growing lewis lung carcinoma (LLC) cells over a 2 day period. However I have been able to grow the cells over a 3 day period but this was less...)
- 12:54, 7 December 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer (top)
- 11:58, 6 November 2012 (hist) (diff) User:Sean E. Lauber (→Useful links)
- 11:54, 6 November 2012 (hist) (diff) User:Sean E. Lauber (→Useful links)
- 11:39, 6 November 2012 (hist) (diff) User:Sean E. Lauber
- 17:42, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II (→Procedure) (top)
- 17:34, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 17:34, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 17:34, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 17:32, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 17:30, 5 November 2012 (hist) (diff) User:Sean E. Lauber (→Education)
- 17:29, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan) (top)
- 17:29, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 17:23, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 17:04, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 17:00, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:55, 5 November 2012 (hist) (diff) Image:Template qPCR.xlsx (uploaded a new version of "Image:Template qPCR.xlsx") (top)
- 16:50, 5 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:45, 5 November 2012 (hist) (diff) Image:Template qPCR.xlsx (uploaded a new version of "Image:Template qPCR.xlsx")
- 16:08, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 16:05, 5 November 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 16:47, 1 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:45, 1 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:44, 1 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:44, 1 November 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 16:42, 1 November 2012 (hist) (diff) N Image:Template qPCR.xlsx
- 16:56, 31 October 2012 (hist) (diff) User:Sean E. Lauber (→Technical guides)
- 16:56, 31 October 2012 (hist) (diff) User:Sean E. Lauber (→Links)
- 15:51, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting) (top)
- 15:51, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:49, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:31, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:31, 30 October 2012 (hist) (diff) N Image:Differential4.jpg (top)
- 15:29, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:28, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:27, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:11, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:10, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:09, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:08, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 15:07, 30 October 2012 (hist) (diff) N Image:Differential3.jpg (top)
- 15:04, 30 October 2012 (hist) (diff) N Image:Differential2.jpg (top)
- 15:03, 30 October 2012 (hist) (diff) N Image:Differential1.jpg (top)
- 14:32, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 14:31, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 14:31, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining (→Differential counting)
- 14:29, 30 October 2012 (hist) (diff) Sean Lauber:Differential cell staining
- 14:18, 30 October 2012 (hist) (diff) N Sean Lauber:Differential cell staining (New page: To differentially stain the cytocentrifuge smears (from BAL), the Hema3 reagent is used (Fisher, 23-123-869). Protocol for staining (each dip is done for 1 second): 1. Dip one slide...)
- 14:10, 30 October 2012 (hist) (diff) User:Sean E. Lauber (→Animal Work)
- 10:46, 26 October 2012 (hist) (diff) Sean Lauber:Heat maps in Excel (top)
- 10:44, 26 October 2012 (hist) (diff) Sean Lauber:Heat maps in Excel
- 10:38, 26 October 2012 (hist) (diff) N Sean Lauber:Heat maps in Excel (New page: Use the following macro: Sub heatmap() Dim myrange As Range Dim colchoice As Integer Set myrange = Application.InputBox("Select a range of cells", rangetocheck, , , , , , 8) For...)
- 10:36, 26 October 2012 (hist) (diff) User:Sean E. Lauber (→Technical)
- 14:40, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping (top)
- 09:38, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:36, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:36, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:19, 16 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus (top)
- 14:50, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:20, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:18, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:16, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:12, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:09, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 10:58, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 10:58, 15 October 2012 (hist) (diff) N Sean Lauber:Isotype ELISA against Adenovirus (New page: Want to determine what antibody isotypes are directed against a specific antigen (in my case it was adenovirus). Wash buffer (10X): 800 ml dH2O 24.23 g Tris 87.66 g NaCl 3.73 g KC...)
- 10:54, 15 October 2012 (hist) (diff) Sean Lauber:Generating Adenovirus Lysate (top)
- 10:54, 15 October 2012 (hist) (diff) N Sean Lauber:Generating Adenovirus Lysate (New page: 1. Hela cells were plated onto a T150 (make 2 plates) 2. When confluent, determine the concentration of cells from the second vessel 3. Wash the cells 3x with PBS, remove the media 4. Infe...)
- 10:54, 15 October 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 10:36, 15 October 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 14:48, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:21, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:16, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:12, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:09, 21 September 2012 (hist) (diff) N Sean Lauber:Kras G12D Genotyping (New page: This protocol makes use of the DirectPCR mouse tail lysis reagent (Viagen, cat 102-T) to obtain template and the hot start Platinum Taq (Invitrogen, cat 10966-026). 1 mm tail ends are co...)
- 12:00, 18 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 13:07, 17 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 11:54, 13 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 12:15, 7 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:49, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:36, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:35, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:35, 13 August 2012 (hist) (diff) N Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer (New page: For 5 animals: 1. Thaw a single vial of 1,000,000 cells 2. Add to 9 ml of warmed 10% FBS DMEM 3. Spin at 10,000 RPM for 3 min 4. Discard supernatant 5. Resuspend to 10 ml 6. Plate this vol...)
- 12:08, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development (top)
- 11:51, 11 July 2012 (hist) (diff) N Image:ELISA1.JPG (top)
- 11:51, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development
- 11:49, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development
- 11:49, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development
- 11:49, 11 July 2012 (hist) (diff) N Image:ELISA1.jpg (top)
- 11:45, 11 July 2012 (hist) (diff) N Sean Lauber:ELISA Development (New page: First you need to determine what reagents you will use for your ELISA. The protocol I use is based off of a generic R&D ELISA. The primary antibody binds to the plate (this should be monoc...)
- 11:20, 11 July 2012 (hist) (diff) Sean Lauber:PCR (top)
- 11:18, 11 July 2012 (hist) (diff) N Sean Lauber:PCR (New page: For each reaction: 18.15 μL nuclease free water 2.5 μL 10X PCR buffer 0.5 μL 10 μM dNTPs 0.75 μL 50 mM MgCl2 1 μL 25 μM primer A 1 μL 25 μM primer B 1 μL ...)
- 11:05, 11 July 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 10:37, 11 April 2012 (hist) (diff) Sean Lauber:Broncho-alveolar Lavage (top)
- 10:37, 11 April 2012 (hist) (diff) N Sean Lauber:Broncho-alveolar Lavage (New page: This procedure explains how to sacrifice the mouse (by bleeding/asphyxiation). Once the mouse is bled and the diaphragm is cut back the mouse is likely dead and the anasthetic nose tube ca...)
- 12:51, 3 April 2012 (hist) (diff) User:Sean E. Lauber (→Protocols)
- 12:51, 3 April 2012 (hist) (diff) Joyce: Preparing RNA from yeast (→Concept) (top)
- 12:49, 3 April 2012 (hist) (diff) Joyce: Extraction of DNA from an agarose gel (→Procedure) (top)
- 12:49, 3 April 2012 (hist) (diff) Joyce: Extraction of DNA from an agarose gel (→Concept)
- 12:46, 3 April 2012 (hist) (diff) N Sean Lauber:Freezing Mammalian Cells (New page: I like to freeze down enough cells to comfortably expand them in a T25. Depending on the cell line this number could vary. I always keep an additional flask with cells to ensure I still ha...) (top)
- 11:53, 3 April 2012 (hist) (diff) User:Sean E. Lauber (→Education & Research Projects)
- 23:08, 2 April 2012 (hist) (diff) Sean Lauber:ImageJ - Threshold Analysis (top)
- 23:06, 2 April 2012 (hist) (diff) N Image:ImageJ-IHC11.png (top)
- 23:05, 2 April 2012 (hist) (diff) N Image:ImageJ-IHC10.png (top)
- 23:03, 2 April 2012 (hist) (diff) N Image:ImageJ-IHC09.png (top)
- 23:02, 2 April 2012 (hist) (diff) N Image:ImageJ-IHC08.png (top)
- 23:01, 2 April 2012 (hist) (diff) N Image:ImageJ-IHC07.png (top)
- 22:49, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Generating Histograms (top)
- 22:48, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Generating Histograms
- 22:46, 2 April 2012 (hist) (diff) N Sean Lauber:AMIRA - Generating Histograms (New page: AMIRA can be used to generate a histogram using a label field. In the previous example we generated a lung label by thoracic segmentation. Now we can generate a histogram of all the differ...)
- 22:45, 2 April 2012 (hist) (diff) N Image:Amira-histogram03.png (top)
- 22:45, 2 April 2012 (hist) (diff) N Image:Amira-histogram02.png (top)
- 22:45, 2 April 2012 (hist) (diff) N Image:Amira-histogram01.png (top)
- 21:52, 2 April 2012 (hist) (diff) N Image:Amira-histogram00.png (top)
- 21:49, 2 April 2012 (hist) (diff) Image:Amira-lung label00.png (uploaded a new version of "Image:Amira-lung label00.png") (top)
- 21:47, 2 April 2012 (hist) (diff) Image:Amira-lung label00.png (uploaded a new version of "Image:Amira-lung label00.png")
- 21:42, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation (top)
- 21:39, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation
- 21:34, 2 April 2012 (hist) (diff) N Image:Amira-lung label09.png (top)
- 21:34, 2 April 2012 (hist) (diff) N Image:Amira-lung label08.png (top)
- 21:32, 2 April 2012 (hist) (diff) N Image:Amira-lung label07.png (top)
- 21:32, 2 April 2012 (hist) (diff) N Image:Amira-lung label06.png (top)
- 21:32, 2 April 2012 (hist) (diff) N Image:Amira-lung label05.png (top)
- 21:27, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation
- 21:24, 2 April 2012 (hist) (diff) N Image:Amira-lung label13.png (top)
- 21:24, 2 April 2012 (hist) (diff) N Image:Amira-lung label12.png (top)
- 21:24, 2 April 2012 (hist) (diff) N Image:Amira-lung label11.png (top)
- 21:24, 2 April 2012 (hist) (diff) N Image:Amira-lung label10.png (top)
- 21:17, 2 April 2012 (hist) (diff) N Image:Amira-lung label04.png (top)
- 21:17, 2 April 2012 (hist) (diff) N Image:Amira-lung label03.png (top)
- 21:17, 2 April 2012 (hist) (diff) N Image:Amira-lung label02.png (top)
- 21:17, 2 April 2012 (hist) (diff) N Image:Amira-lung label01.png (top)
- 21:17, 2 April 2012 (hist) (diff) N Image:Amira-lung label00.png
- 15:03, 2 April 2012 (hist) (diff) Image:Amira-lung label2.png (uploaded a new version of "Image:Amira-lung label2.png") (top)
- 14:59, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation
- 14:58, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation
- 14:58, 2 April 2012 (hist) (diff) N Image:Amira-lung label2.png
- 14:57, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation
- 14:56, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation
- 14:55, 2 April 2012 (hist) (diff) Image:Amira-lung label1.png (uploaded a new version of "Image:Amira-lung label1.png") (top)
- 14:52, 2 April 2012 (hist) (diff) Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation
- 14:52, 2 April 2012 (hist) (diff) Image:Amira-lung label1.png (uploaded a new version of "Image:Amira-lung label1.png")
- 14:49, 2 April 2012 (hist) (diff) Image:Amira-lung label1.png (uploaded a new version of "Image:Amira-lung label1.png")
- 14:48, 2 April 2012 (hist) (diff) Image:Amira-lung label1.png (uploaded a new version of "Image:Amira-lung label1.png")
- 14:45, 2 April 2012 (hist) (diff) N Sean Lauber:AMIRA - Creating Label Fields by Lung Segmentation (New page: In order to evaluate changing densities in the lung of an affected animal, you need to separate the lung portion of the CT scan from the rest of the CT scan. This is done by creating a lun...)
- 14:41, 2 April 2012 (hist) (diff) N Image:Amira-lung label1.png
- 12:29, 2 April 2012 (hist) (diff) Sean Lauber:ImageJ - Threshold Analysis
- 12:09, 2 April 2012 (hist) (diff) Sean Lauber:ImageJ - Threshold Analysis
- 11:57, 2 April 2012 (hist) (diff) Sean Lauber:ImageJ - Threshold Analysis
- 11:55, 2 April 2012 (hist) (diff) Sean Lauber:ImageJ - Threshold Analysis
- 11:53, 2 April 2012 (hist) (diff) Sean Lauber:ImageJ - Threshold Analysis
- 10:56, 2 April 2012 (hist) (diff) N Sean Lauber:ImageJ - Threshold Analysis (New page: Image:ImageJ.JPG)
- 10:54, 2 April 2012 (hist) (diff) N Image:ImageJ.JPG (top)
- 12:05, 30 March 2012 (hist) (diff) N Sean Lauber:Using the 7900 HT qPCR Machine (New page: #Open SDS (v.??) #Create a new file (File/New), choose Relative Quantification #Add Detector (18S is VIC, all other probes are FAM based) #) (top)
- 11:49, 30 March 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 11:45, 30 March 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 11:45, 30 March 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 11:37, 30 March 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 11:34, 30 March 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 11:33, 30 March 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 11:32, 30 March 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 11:25, 30 March 2012 (hist) (diff) Sean Lauber:qRT-PCR (TaqMan)
- 11:23, 30 March 2012 (hist) (diff) N Image:96-well Taqman.JPG (top)
- 11:21, 30 March 2012 (hist) (diff) N Sean Lauber:qRT-PCR (TaqMan) (New page: I typically use 10 ng cDNA/Taqman reaction and I deliver 5 μL, so you want a cDNA concentration of 2 ng/μL. For this example I am going to pretend I have 2 samples (X and Y) and I want t...)
- 10:58, 30 March 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 10:57, 30 March 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 10:56, 30 March 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II
- 10:55, 30 March 2012 (hist) (diff) Sean Lauber:Reverse Transcription using Superscript II (→Procedure)
- 10:54, 30 March 2012 (hist) (diff) N Sean Lauber:Reverse Transcription using Superscript II (New page: ===Procedure=== Using superscript II (Invitrogen CAT# 18064-022) First you need to determine how much cDNA you want to generate. How much will you need for TaqMan? I generally use 10 ng/...)
- 10:27, 30 March 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 10:25, 30 March 2012 (hist) (diff) N Sean Lauber:DNase Treatment Using DNA-Free (New page: ===Procedure=== Using Ambion DNA-free (CAT# 1906) #Bring your RNA solution up to a final volume of 21.5 μL. <b> Be sure that your RNA solution contains less than 10 μg of total RNA.</b...) (top)
- 10:17, 30 March 2012 (hist) (diff) Sean Lauber:DNA Extraction Using Trizol (→Procedure) (top)
- 10:17, 30 March 2012 (hist) (diff) Sean Lauber:DNA Extraction Using Trizol (→Na Citrate)
- 10:16, 30 March 2012 (hist) (diff) N Sean Lauber:DNA Extraction Using Trizol (New page: ===Procedure=== #Remove media on cells and add 1 ml of Trizol (regardless of plate size). <b> DO THIS ON THE BENCH AND NOT IN THE TISSUE CULTURE HOOD</b> #Scrape cells and pipette into a ...)
- 10:02, 30 March 2012 (hist) (diff) User:Sean E. Lauber (→Education & Research Projects)
- 10:01, 30 March 2012 (hist) (diff) User:Sean E. Lauber (→Education)
- 10:00, 30 March 2012 (hist) (diff) User:Sean E. Lauber (→Research Projects)
- 09:59, 30 March 2012 (hist) (diff) User:Sean E. Lauber (→Education)
- 09:59, 30 March 2012 (hist) (diff) User:Sean E. Lauber (→Education)
- 19:03, 29 March 2012 (hist) (diff) Lauber:The Role of gp130 Cytokines IL-6 and OSM on Lung Tumor Development in a Mouse Model for Lung Adenocarcinoma (top)
- 18:51, 29 March 2012 (hist) (diff) N Lauber:The Role of gp130 Cytokines IL-6 and OSM on Lung Tumor Development in a Mouse Model for Lung Adenocarcinoma (New page: k)
- 18:50, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Research interests)
- 14:56, 29 March 2012 (hist) (diff) Sean Lauber:RNA Extraction Using Trizol (→Procedure) (top)
- 14:54, 29 March 2012 (hist) (diff) Sean Lauber:RNA Extraction Using Trizol
- 14:44, 29 March 2012 (hist) (diff) Sean Lauber:RNA Extraction Using Trizol (→Procedure)
- 14:35, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Animal Work)
- 14:33, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Mammalian Cell Culture)
- 14:32, 29 March 2012 (hist) (diff) N Sean Lauber:RNA Extraction Using Trizol (New page: ===Procedure=== For extraction of RNA from cells on a 6-well plate)
- 14:29, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Joyce Lab Protocols)
- 14:29, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Mammalian Cell Culture)
- 14:27, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 14:15, 29 March 2012 (hist) (diff) Sean Lauber:Cytokeratin IHC staining (→Chromogen/substrate solution) (top)
- 14:14, 29 March 2012 (hist) (diff) Sean Lauber:Cytokeratin IHC staining (→Solutions required)
- 14:14, 29 March 2012 (hist) (diff) Sean Lauber:Cytokeratin IHC staining (→1:500 pan-cytokeratin)
- 14:14, 29 March 2012 (hist) (diff) Sean Lauber:Cytokeratin IHC staining (→Solutions required)
- 14:11, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Richards Lab Protocols)
- 14:10, 29 March 2012 (hist) (diff) N Sean Lauber:Cytokeratin IHC staining (New page: ==Protocol== #Cut 3 μM sections, mount on slides, air dry overnight #Place slides at 60*C for 45 min #Place slides in xylene I for 5 min, then to xylene II for 5 min, then to xylene III...)
- 14:10, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 14:10, 29 March 2012 (hist) (diff) N Lauber:Cytokeratin IHC staining (New page: ==Protocol== #Cut 3 μM sections, mount on slides, air dry overnight #Place slides at 60*C for 45 min #Place slides in xylene I for 5 min, then to xylene II for 5 min, then to xylene III...) (top)
- 14:10, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 14:06, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Technical)
- 14:02, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Links)
- 14:01, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Joyce Lab Protocols)
- 14:00, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Joyce Lab Protocols)
- 13:59, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Technical)
- 13:58, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Richards Lab Protocols)
- 13:45, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 13:43, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Richards Lab Protocols)
- 13:36, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Richards Lab Protocols)
- 10:58, 29 March 2012 (hist) (diff) Richards:Cytokeratin IHC staining (→Methanol solution) (top)
- 10:56, 29 March 2012 (hist) (diff) Richards:Cytokeratin IHC staining (→Protocol)
- 10:56, 29 March 2012 (hist) (diff) Richards:Cytokeratin IHC staining (→Protocol)
- 10:54, 29 March 2012 (hist) (diff) N Richards:Cytokeratin IHC staining (New page: ==Protocol== ===Deparaffinize=== 1. Cut 3 μM sections, mount on slides, air dry overnight 2. Place slides at 60*C for 45 min 3. Place slides in xylene I for 5 min, then to xylene II for ...)
- 10:53, 29 March 2012 (hist) (diff) N Richards: Cytokeratin IHC staining (New page: ==Protocol== ===Deparaffinize=== 1. Cut 3 μM sections, mount on slides, air dry overnight 2. Place slides at 60*C for 45 min 3. Place slides in xylene I for 5 min, then to xylene II for ...) (top)
- 10:18, 29 March 2012 (hist) (diff) User:Sean E. Lauber (→Protocols)
- 22:08, 26 March 2012 (hist) (diff) User:Sean E. Lauber (→My brilliant contributions)
- 22:07, 26 March 2012 (hist) (diff) User:Sean E. Lauber (→Education)
- 22:03, 26 March 2012 (hist) (diff) User:Sean E. Lauber (→Publications)
- 22:03, 26 March 2012 (hist) (diff) User:Sean E. Lauber (→Joyce May-Dec 2009)
- 22:02, 26 March 2012 (hist) (diff) User:Sean E. Lauber (→Piekny Sept - 2009)
- 02:00, 2 January 2010 (hist) (diff) User:Sean E. Lauber (→Contact Info)
- 13:39, 22 November 2009 (hist) (diff) User:Sean E. Lauber (→Fun stuff, useful links)
- 23:30, 3 November 2009 (hist) (diff) User:Sean E. Lauber (→Protocols and general information)
- 11:08, 2 November 2009 (hist) (diff) User:Sean E. Lauber (→Protocols and general information)
- 02:25, 23 October 2009 (hist) (diff) User:Sean E. Lauber (→Fun stuff, useful links)
- 02:25, 23 October 2009 (hist) (diff) User:Sean E. Lauber (→Fun stuff, useful links)
- 23:44, 27 September 2009 (hist) (diff) User:Sean E. Lauber (→Fun stuff, useful links)
- 12:15, 21 September 2009 (hist) (diff) User:Sean E. Lauber (→Piekny Sept - 2009)
- 12:14, 21 September 2009 (hist) (diff) N Piekny: splitting cells (New page: ==Concept== Tissue culture cells need to be "split" to keep them alive. The process involves taking a portion of the cells and resuspending them in fresh culture media so they can grow. I...) (top)
- 11:59, 21 September 2009 (hist) (diff) User:Sean E. Lauber (→My brilliant contributions)
- 01:18, 20 September 2009 (hist) (diff) User:Sean E. Lauber (→Joyce May-Dec 2009)
- 01:18, 20 September 2009 (hist) (diff) N Joyce: Ligation (New page: http://www.protocol-online.org/biology-forums/ligation.html Read more from * Tech Tips 5 DNA Ligation Tips by Nick on September 4, 2007 no_clones.JPGDNA ligations can be frustratin...) (top)
- 16:12, 15 September 2009 (hist) (diff) Lauber: I won't forget (top)
- 00:05, 15 September 2009 (hist) (diff) Joyce: T-tailing pBSKS (→Notes) (top)
- 23:58, 14 September 2009 (hist) (diff) Joyce:Transformation (top)
(Latest | Earliest) View (newer 250) (older 250) (20 | 50 | 100 | 250 | 500)
