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  • 12:45, 23 January 2013 (hist) (diff) m Yu:Procedures(link) (top)
  • 12:43, 23 January 2013 (hist) (diff) Nm Yu:Bead Cleaning(Cleaning) (top)
  • 20:03, 1 December 2012 (hist) (diff) Nm Yu:TESSX(TESSX) (top)
  • 19:59, 1 December 2012 (hist) (diff) m Yu:Procedures(link)
  • 19:58, 1 December 2012 (hist) (diff) N Yu:STET(STET) (top)
  • 19:53, 1 December 2012 (hist) (diff) Nm Yu:Yeast miniprep(Yeast minipreps) (top)
  • 18:25, 1 December 2012 (hist) (diff) m Template:Yu(links) (top)
  • 16:21, 26 January 2012 (hist) (diff) m Yu:Super SPO(template) (top)
  • 16:20, 26 January 2012 (hist) (diff) m Yu:Procedures(link)
  • 16:20, 26 January 2012 (hist) (diff) N Yu:Super SPO(SUPER SPO)
  • 15:16, 29 December 2011 (hist) (diff) m Yu:SOC(Fixed) (top)
  • 01:05, 1 December 2011 (hist) (diff) m Yu(top)
  • 18:07, 20 September 2011 (hist) (diff) m Yu:TBS(typo) (top)
  • 18:07, 20 September 2011 (hist) (diff) m Yu:TBS
  • 10:18, 1 September 2011 (hist) (diff) m Yu:PLAAC(top)
  • 10:17, 1 September 2011 (hist) (diff) m Yu:PLAAC
  • 10:15, 1 September 2011 (hist) (diff) m Yu:PLAAC
  • 14:05, 28 July 2011 (hist) (diff) Yu
  • 00:42, 7 July 2011 (hist) (diff) m Yu
  • 17:35, 9 May 2011 (hist) (diff) m Yu:Procedures(Link)
  • 17:34, 9 May 2011 (hist) (diff) N Yu:Genomic prep(Genomic Prep) (top)
  • 19:02, 7 May 2011 (hist) (diff) m Yu:Dropout Media(Fix) (top)
  • 19:01, 7 May 2011 (hist) (diff) N Yu:Dropout Media(DO Media)
  • 19:11, 22 February 2011 (hist) (diff) m Yu:Procedures
  • 19:11, 22 February 2011 (hist) (diff) m Yu:SOC(Touchup)
  • 17:16, 28 January 2011 (hist) (diff) m Yu(Comic)
  • 15:36, 9 September 2010 (hist) (diff) m Yu:Publications(top)
  • 19:44, 26 August 2010 (hist) (diff) m Yu:Publications
  • 16:41, 8 August 2010 (hist) (diff) m Yu:Procedures(competent cells)
  • 16:39, 8 August 2010 (hist) (diff) N Yu:Competent Cells(E Coli Competent cells) (top)
  • 11:48, 8 June 2010 (hist) (diff) m Yu:e coli transform(fix) (top)
  • 14:00, 26 May 2010 (hist) (diff) m Yu
  • 13:32, 22 May 2010 (hist) (diff) m Yu:Dialysis(fix) (top)
  • 13:31, 22 May 2010 (hist) (diff) m Yu:Dialysis(dialysis)
  • 10:06, 18 May 2010 (hist) (diff) m Yu:Dialysis(typo)
  • 12:28, 17 May 2010 (hist) (diff) m Yu:Procedures(.)
  • 12:27, 17 May 2010 (hist) (diff) N Yu:Dialysis(dialysis)
  • 18:47, 14 May 2010 (hist) (diff) m Labs(North America)
  • 18:41, 14 May 2010 (hist) (diff) m Yu:PBS(fix) (top)
  • 20:17, 9 May 2010 (hist) (diff) m Yu
  • 20:07, 9 May 2010 (hist) (diff) m Yu:TBS(sp)
  • 20:06, 9 May 2010 (hist) (diff) m Yu:Procedures(TBS)
  • 20:05, 9 May 2010 (hist) (diff) m Yu:TBS
  • 19:57, 9 May 2010 (hist) (diff) N Yu:TBS(TBS)
  • 19:53, 9 May 2010 (hist) (diff) m Yu:PBS(fixed mMs)
  • 17:21, 4 May 2010 (hist) (diff) m Yu:Transformation(top)
  • 17:19, 4 May 2010 (hist) (diff) m Yu:Procedures(TOPOTA)
  • 17:14, 4 May 2010 (hist) (diff) m Yu:Transformation
  • 17:12, 4 May 2010 (hist) (diff) m Yu:LiOAc(top)
  • 12:44, 16 April 2010 (hist) (diff) m Yu:CoIP(Altered TEV for eppendorf tubes)
  • 18:05, 26 February 2010 (hist) (diff) Yu:Transformation
  • 14:58, 24 December 2009 (hist) (diff) m Yu:TEV Cleavage Buffer(fixed typo) (top)
  • 15:46, 22 December 2009 (hist) (diff) N Yu:CEB(CEB) (top)
  • 15:37, 22 December 2009 (hist) (diff) Yu:IPP(fixed) (top)
  • 15:33, 22 December 2009 (hist) (diff) Yu:TEV Cleavage Buffer(fixed)
  • 15:28, 22 December 2009 (hist) (diff) Yu:CBB(fixed) (top)
  • 20:21, 17 December 2009 (hist) (diff) m Yu:Lysis(top)
  • 20:19, 17 December 2009 (hist) (diff) m Yu:Lysis
  • 20:46, 5 December 2009 (hist) (diff) m Yu:Lab Members(top)
  • 20:45, 5 December 2009 (hist) (diff) m Yu:Lab Members
  • 20:45, 5 December 2009 (hist) (diff) N Image:Yu Chris.jpg(top)
  • 13:28, 2 December 2009 (hist) (diff) m Yu:Western(top)
  • 13:28, 2 December 2009 (hist) (diff) m Yu:Western
  • 13:27, 2 December 2009 (hist) (diff) Yu:Western(Added immunoblotting)
  • 13:05, 2 December 2009 (hist) (diff) m Yu:CoIP(Precipitating Protein)
  • 13:04, 2 December 2009 (hist) (diff) Yu:CoIP(Added precipitation step)
  • 14:25, 14 October 2009 (hist) (diff) m Yu
  • 14:17, 5 October 2009 (hist) (diff) m Yu
  • 11:22, 2 October 2009 (hist) (diff) N Yu:Harvest cells(cell harvest) (top)
  • 11:09, 2 October 2009 (hist) (diff) m Yu:CoIP
  • 17:28, 26 September 2009 (hist) (diff) m Yu
  • 17:24, 26 September 2009 (hist) (diff) m Yu
  • 14:32, 26 September 2009 (hist) (diff) m Yu:CoIP
  • 13:37, 26 September 2009 (hist) (diff) m Yu:TST(top)
  • 13:37, 26 September 2009 (hist) (diff) m Yu:TEV Cleavage Buffer(TEV)
  • 13:28, 26 September 2009 (hist) (diff) N Yu:TST(TST)
  • 13:24, 26 September 2009 (hist) (diff) m Yu:CoIP(TEV)
  • 12:27, 26 September 2009 (hist) (diff) m Yu:CoIP
  • 12:26, 26 September 2009 (hist) (diff) m Yu:Procedures
  • 12:25, 26 September 2009 (hist) (diff) m Yu:CoIP
  • 12:23, 26 September 2009 (hist) (diff) N Yu:CoIP(CoIP)
  • 13:15, 25 September 2009 (hist) (diff) m Yu:NP40 Buffer(top)
  • 17:07, 22 September 2009 (hist) (diff) m Category:Yu Solutions(top)
  • 17:05, 22 September 2009 (hist) (diff) m Category:Yu Lab(top)
  • 17:04, 22 September 2009 (hist) (diff) m Yu:Denhart's Solution(Redirecting to Yu:Northern solns#100x Denhardt.27s) (top)
  • 17:03, 22 September 2009 (hist) (diff) m Yu:AQUEOUS PRe Hyb BUFFER(Redirecting to Yu:Northern solns#Hybridization Buffers) (top)
  • 17:02, 22 September 2009 (hist) (diff) m Yu:AQUEOUS Hyb BUFFER(Redirecting to Yu:Northern solns#Hybridization Buffers) (top)
  • 17:00, 22 September 2009 (hist) (diff) m Yu:TAE(top)
  • 17:00, 22 September 2009 (hist) (diff) m Yu:50X TAE(Redirecting to Yu:TAE) (top)
  • 16:59, 22 September 2009 (hist) (diff) Nm Category:Yu Lab(New page: {{Template:Yu}})
  • 16:57, 22 September 2009 (hist) (diff) m Yu:Northern solns(top)
  • 16:57, 22 September 2009 (hist) (diff) m Yu:20X SSC(Redirecting to Yu:Northern solns#20x SSC) (top)
  • 16:56, 22 September 2009 (hist) (diff) m Yu:PBS
  • 16:55, 22 September 2009 (hist) (diff) m Yu:10X PBS(Redirecting to Yu:PBS) (top)
  • 16:53, 22 September 2009 (hist) (diff) m Yu:SDS PAGE Sample Buffer(top)
  • 16:52, 22 September 2009 (hist) (diff) m Yu
  • 16:52, 22 September 2009 (hist) (diff) m Template:Yu
  • 16:50, 22 September 2009 (hist) (diff) N Category:Yu Solutions(New page: Category:Yu Lab)
  • 16:50, 22 September 2009 (hist) (diff) m Yu:Western Transfer Buffer(category) (top)
  • 16:48, 22 September 2009 (hist) (diff) m Yu:WESTERN TRANSFER BUFFER FOR 1 L(Redirecting to Yu:Western Transfer Buffer) (top)
  • 16:53, 18 September 2009 (hist) (diff) N Yu:SOC(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for SOC'''. |- |For 100ml |- |1. 2g tryptone (2%w/v) |- |2. 0.5 g yeast extract (0.5%w/v) |- |3. 330μL 3M NaCl(10mM...)
  • 16:48, 18 September 2009 (hist) (diff) Yu:RNAse
  • 16:47, 18 September 2009 (hist) (diff) N Yu:RNAse(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for RNAse(10mg/ml)'''. |- |Per 50ml: |- |1. 50ml MiliQ water |- |2. 0.5g RNAseA |- |3. 0.5ml TRis HCl(1M stock, pH 7.5...)
  • 16:43, 18 September 2009 (hist) (diff) N Yu:RIPA-Buffer(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for RIPA Buffer'''. |- | For 250ml: |- |1. 50 mM Tris pH 8.0( 12.5ml of 1M Stock) |- |2. 150 mM NaCl(7.5 l of 5M Stock)...)
  • 16:39, 18 September 2009 (hist) (diff) N Yu:Potassium Acetate(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for Potassium Acetate (For Alkaline Lysis) '''. |- |To 60ml of 5M Potassium acetate: |- |1. Add 11.5 ml of glacial ac...)
  • 16:35, 18 September 2009 (hist) (diff) N Yu:PLAAC(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for PLAAC(Protease inhibitor cocktail for yeast)'''. |- | 1:1:1:1 of lab stock=1000x |- | Can add PMSF(1mM final) |- | ...)
  • 16:31, 18 September 2009 (hist) (diff) N Yu:PBS KMT(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for PBS KMT '''. |1. 20mL PBS (10X) |- |2. 600 μL 1M KCl=3mM Final concentration |- |3. 500μL 1M MgCl<sub>2</sub>=2.5...)
  • 16:22, 18 September 2009 (hist) (diff) N Yu:IPTG(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for IPTG(Powder stored at -20<sup>0</sup>C)'''. |- |Isopropylthio-β-<sub>D</sub>-galactoside(m.w=238.3) |- |1. Dissol...) (top)
  • 16:15, 18 September 2009 (hist) (diff) N Yu:FORMAMIDE(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for FORMAMIDE LOADING BUFFER FOR ACYLAMIDE GEL'''. |- | 0.05%(w/v)bromophenol blue, |- | 0.05%(w/v) xylene cyanol |- |...)
  • 16:08, 18 September 2009 (hist) (diff) N Yu:Denhart's Solution(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for DENHARDT'S Solution( 100X)'''. |1. 2g Ficoll |- |2. 2g polyvinlypyrrolidone |- |3. 2g BSA(Sigma fraction V) |- |4....)
  • 16:04, 18 September 2009 (hist) (diff) N Yu:Destain Second(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for DESTAIN (Second, slow) '''. |- |7.5% Methanol, 5% Acetic Acid |- | Destain until desired background is showing |...) (top)
  • 15:59, 18 September 2009 (hist) (diff) N Yu:Destain First(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for Destain (First) '''. |- |45% Methanol, 10% acetic acid |- |1. Destain for 1/2 hr first (With a couple of changes in...) (top)
  • 15:55, 18 September 2009 (hist) (diff) N Yu:Cycloheximide(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for Cycloheximide(1000x) '''. |1. Make 100mg/ml in ethanol, dark,-20<sup>0</sup>C |- |2. Use in vivo protein methylatio...) (top)
  • 15:52, 18 September 2009 (hist) (diff) N Yu:Coomasie blue(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for Coomassie Blue( for mass spec,too)'''. |1. 45% Methanol |- |2. 10% Acetic Acid |- |3. Water |- |4. Brilliant Blue G...) (top)
  • 15:46, 18 September 2009 (hist) (diff) N Yu:Chloramphenicol(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for Chloramphenicol,1000X'''. |1. 1000X=40mg/ml |- |2. make ethanol, storage, store at -20<sup>0</sup>C |) (top)
  • 15:42, 18 September 2009 (hist) (diff) N Yu:ChIP wash buffer(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for ChIP Wash Buffer (For 50ml)'''. |1. 0.5ml 1M Tris pH 8.0 |- |2. 3.13 ml 4M LiCl |- |3. 100 ul 0.5M EDTA |- |4. 250...) (top)
  • 15:35, 18 September 2009 (hist) (diff) N Yu:ChIP elution buffer(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for ChIP elution buffer for 50ml'''. |1. 2.5ml 1M Tris |- |2. 1 ml of 0.5M EDTA |- |3. 5ml of 10% SDS |- |4. 41.5ml wat...) (top)
  • 15:32, 18 September 2009 (hist) (diff) N Yu:ChIP Diluent(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for ChIP diluent'''. |1. 0.143M NaCl( 14.3 ml of 5M Stock) |- |2. 1.43 nM EDTA(1.43 ml 0.5M Stock) |- |3. 71.43 mM HEPE...) (top)
  • 15:29, 18 September 2009 (hist) (diff) N Yu:AQUEOUS PRe Hyb BUFFER(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for Aqueous Pre-Hyb Buffer(for oligo probe) '''. |1. 30ml 20X SSc |- |2. 2 ml 50X Denhardt's |- |3. 2.5 ml 20% SDS |- |...)
  • 15:26, 18 September 2009 (hist) (diff) N Yu:AQUEOUS Hyb BUFFER(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for Aqueous Hyb buffer (for oligo probe) '''. |1. 30ml 20X SSC |- |2. 2ml 50X Denhardt's |- |3. 0.5ml 20% SDS |- |4. 67...)
  • 15:23, 18 September 2009 (hist) (diff) N Yu:ACTINOMYCIN D(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for ACTINOMYCIN D'''. |1. Dissolve 20mg OF ACTINOMYCIN D in 4ml of 100% ethanol. |- |2. Read the OD<sub>440</sub> of ...) (top)
  • 15:15, 18 September 2009 (hist) (diff) N Yu:PMSF(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 200mM PMSF (Phenylmethyl-Sulfonyl Fluoride)'''. |1. Dissolve PMSF in ethanol at a concentration of 0.035g/ml in an ...)
  • 15:12, 18 September 2009 (hist) (diff) N Yu:50X TAE(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 50X TAE'''. | |The final concentration of reagents in 50X TAE is 2M Tris,2M Acetic acid, and 50mM EDTA. Per Liter: ...)
  • 15:08, 18 September 2009 (hist) (diff) N Yu:20X SSPE(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 20X SSPE '''. |1. Dissolve 175.3g of NaCl , 27.6g of NaH<sub>2</sub>PO<sub>4</sub>.H<sub>2</sub>O and 7.4g of EDTA ...) (top)
  • 15:03, 18 September 2009 (hist) (diff) N Yu:20X SSC(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 20X SSC'''. |1. Dissolve 175.3 g of NaCl, 88.2g of Sodium citrate in 800ml of H<sub>2</sub>O. |- |2. Adjust the p...)
  • 14:59, 18 September 2009 (hist) (diff) N Yu:RAFFINOSE(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 20% RAFFINOSE'''. |1. 25g into 125ml of water |- |2. '''Sterile filter''' the solution |- |* For use in Gal induct...)
  • 14:56, 18 September 2009 (hist) (diff) N Yu:GALACTOSE(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 20% GALACTOSE'''. |1. 25g into 125ml (final volume)dd H<sub>2</sub>O |- |2. Add heat to solubilize during stirring ...) (top)
  • 14:54, 18 September 2009 (hist) (diff) Yu:GLUCOSE(top)
  • 14:52, 18 September 2009 (hist) (diff) N Yu:GLUCOSE(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 20% GALACTOSE'''. |1. 25g into 125ml (final volume )dd H<sub>2</sub>O |- |2. Add heat to solubilize during stirrin...)
  • 14:49, 18 September 2009 (hist) (diff) N Yu:SDS(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 10 % SDS'''. |1. Dissolve 100 g of electrophoresis-grade SDS in 900ml of H<sub>2</sub>O. |- |2. Heat to 68<sup>0</s...)
  • 14:45, 18 September 2009 (hist) (diff) N Yu:AMMONIUM PERSULFATE(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 10%Ammonium Persulfate(w/v)'''. |1. 5g of ammonium persulfae (stored in a dessicated tuppleware at RT) |- |2. Mill...) (top)
  • 14:42, 18 September 2009 (hist) (diff) N Yu:AMMONIUM ACETATE(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 10M Ammonium Acetate'''. |1. Dissolve 770g of ammonium acetate in 800ml of water |- |2. Adjust the volume to 1 lite...) (top)
  • 14:39, 18 September 2009 (hist) (diff) N Yu:WESTERN TRANSFER BUFFER FOR 1 L(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 10X Western Transfer Buffer (use at 0.5X!)-For 1L.''' |1. 30.3 g Tris |- |2. 144.2g glycine |-)
  • 14:35, 18 September 2009 (hist) (diff) N Yu:SDS PAGE RUNNING BUFFER(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 10X SDS_PAGE Running Buffer '''. |1. 30.3 g Tris base |- |2. 144.2 g Glycine |- |3. 10g SDS |- |4. DO NOT pH IT)
  • 14:32, 18 September 2009 (hist) (diff) N Yu:10X PBS(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 10X PBS (For making 1 liter )'''. |- Diluter to 1X for use |1. 80g NaCl |- |2. 2g KCl |- |3. 14.4g Na<sub>2</sub>HP...)
  • 14:25, 18 September 2009 (hist) (diff) N Yu:MOPS(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 10X MOPS'''. |1. Per 1 Liter: Dissolve 41.86g MOPS. |- |2. 6.80g Sodium Acetate |- |3. 3.72g EDTA |- |4. pH shoul...) (top)
  • 14:22, 18 September 2009 (hist) (diff) N Yu:DNA loading Dye(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 6X DNA Loading Dye. For making 50ml (make it inside a 50 ml falcon tube)'''. |1. 30ml 50% Glycerol |- |2. 50mg-Brom...) (top)
  • 14:18, 18 September 2009 (hist) (diff) N Yu:TBE per L(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 5X TBE (Tris-Borate buffer)-per L'''. |1. 54 g Tris Base. |- |2. 27.5 boric acid |- |3. 20ml 0.5m EDTA(pH 8.0) |)
  • 14:15, 18 September 2009 (hist) (diff) N Yu:NaCl(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 5M NaCl'''. |1. Dissolve 292.2 g of NaCl in 800ml of H<sub>2</sub>O. |- |2. Adjust the volume to 1 liter with H<sub...) (top)
  • 14:12, 18 September 2009 (hist) (diff) N Yu:SDS PAGE SAMPLE BUFFER(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 3X SDS_PAGE Sample Buffer'''. |1. Make it in a 15ml Falcon tube . |- |2. 2ml glycerol |- |3. 4ml 10% SDS |- |4. 0.3...)
  • 14:07, 18 September 2009 (hist) (diff) N Yu:Glycine/200mM Tris(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 3M Glycine /20mM Tris (For ChIP experiment)'''. |1. 2.433g Tris. |- |2. 225.21g glycine. |- |3. up to 1L |- |4. Hea...) (top)
  • 13:58, 18 September 2009 (hist) (diff) N Yu:Sodium Acetate(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 3M Sodium Acetate, PpH 5.2 and pH 7.0'''. |1. Dissolve 408.1g of sodium Acetate.3H<sub>2</sub>O in <700ml of H<sub>...)
  • 13:53, 18 September 2009 (hist) (diff) Yu:PBSMT
  • 13:52, 18 September 2009 (hist) (diff) N Yu:FA Lysis buffer(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1M Tris( Various pH)'''. |1. 100mM HEPES(40ml of 0.5M stock,pH 7.5). |- |2. 300mM NaCl(12ml of 5M) |- |3. 2mM EDTA(...) (top)
  • 13:47, 18 September 2009 (hist) (diff) N Yu:CAPS(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 10X CAPS Transfer Buffer'''. |1. 100mM CAPS (pH 11). |- |2. 10% MeOH( Fresh) |- |3. Use at 1X |) (top)
  • 13:45, 18 September 2009 (hist) (diff) N Yu:PBSMT(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1X PBSMT'''. |1. Add 100ml 10X PBS. |- |2. 1ml 3M KCl |- |3. 2.5ml 1M MgCl<sub>2</sub> |- |4. 50ml 10% Triton X-100...)
  • 13:34, 18 September 2009 (hist) (diff) Yu:Tris-HCl
  • 13:30, 18 September 2009 (hist) (diff) Yu:Tris
  • 13:26, 18 September 2009 (hist) (diff) N Yu:Tris-HCl(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1.5 M Tris-HCl, pH 8.8'''. |1. Add 54.45 g of Tris base into 150ml distilled water. |- |2. . |- |3. Adjust he PpH t...)
  • 13:25, 18 September 2009 (hist) (diff) Yu:EDTA(top)
  • 13:24, 18 September 2009 (hist) (diff) N Yu:Tris(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1M Tris( Various pH)'''. |1. Dissolve 121.1g of Tris base in 800ml of H<sub>2</sub>O. |- |2. Adjust the pH to the ...)
  • 13:15, 18 September 2009 (hist) (diff) N Yu:KOAc(New page: {| border="0" cellpadding="10" |+'''Solution recipe for 1M Potassium acetate, pH 7.5'''. |1. Dissolve 9.82 g of Potassium acetate in 90ml of pure H<sub>2</sub>0 (Milli-Q or equivalent). |-...) (top)
  • 13:09, 18 September 2009 (hist) (diff) Yu:EDTA
  • 13:03, 18 September 2009 (hist) (diff) N Yu:MgCl2(New page: {| border="0" cellpadding="10" |+'''Solution recipe for 1M Magnesium Cloride (MgCl<sub>2</sub>)'''. |1. Dissolve 203.3g of MgCl<sub>3</sub>.6H<sub>2</sub>O in 800ml of pure H<sub>2</sub>O ...) (top)
  • 12:59, 18 September 2009 (hist) (diff) N Yu:CaCl2(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1M CaCl<sub>2</sub>'''. |1. Dissolve 54 g of CaCl<sub>2</sub>.6H<sub>2</sub>O in 200ml of pure H<sub>2</sub>O (Mill...) (top)
  • 12:53, 18 September 2009 (hist) (diff) N Yu:DDT(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1M DDT (dithiothreitol), power stored at -20<sup>o</sup>C'''. |1. Dissolve 3.09 g of DTT in 20ml of 0.01M Sodium Ac...) (top)
  • 12:48, 18 September 2009 (hist) (diff) N Yu:MgOAc(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1M Magnesium acetate'''. |1. Dissolve 214.46g of magnesium acetate.4H<sub>2</sub>O in 800ml of H<sub>2</sub>O |- |2...) (top)
  • 12:43, 18 September 2009 (hist) (diff) Yu:EDTA
  • 12:34, 18 September 2009 (hist) (diff) N Yu:EDTA(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for .5M EDTA'''. |1. Add 186.1g disodium ethylenediaminetetraacetate to H<sub>2</sub>O and bring volume to 800 mL. |- |...)
  • 17:54, 10 July 2009 (hist) (diff) m Template:Yu
  • 18:36, 15 June 2009 (hist) (diff) Yu:Lab Members(real pictures)
  • 18:35, 15 June 2009 (hist) (diff) N Image:NicoleG.jpg(top)
  • 18:34, 15 June 2009 (hist) (diff) N Image:EricM.jpg(top)
  • 18:34, 15 June 2009 (hist) (diff) N Image:Neelu.jpg(top)
  • 18:33, 15 June 2009 (hist) (diff) N Image:MCYu.jpg(top)
  • 18:32, 15 June 2009 (hist) (diff) N Image:ChelseaC.jpg(top)
  • 15:15, 10 June 2009 (hist) (diff) m Yu:Procedures
  • 15:14, 10 June 2009 (hist) (diff) N Yu:Lysis(quick lyse)
  • 14:13, 9 June 2009 (hist) (diff) Yu:Research(content) (top)
  • 20:07, 6 June 2009 (hist) (diff) m Template:Yu
  • 17:36, 6 June 2009 (hist) (diff) Nm Yu:PBS(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1L 10x Phosphate-Buffered Saline. Dilute to 1x for use.''' |width="80%" valign="top"|1. Measure 700mL millipore wat...)
  • 17:31, 6 June 2009 (hist) (diff) Nm Yu:SDS PAGE Buffer(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1L 10x SDS-PAGE Buffer. Dilute to 1x for use.''' |width="80%" valign="top"|1. Measure 700mL millipore water into a ...)
  • 17:28, 6 June 2009 (hist) (diff) N Yu:Western Transfer Buffer(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1L 10x Western Transfer Buffer. Dilute to .5x for use.''' |width="80%" valign="top"|1. Measure 700mL millipore wat...)
  • 17:01, 6 June 2009 (hist) (diff) m Yu:Publications(Replacing page with '{{Template:Yu}} <biblio> #1 pmid=17189192 #2 pmid=17158743 #3 pmid=17130867 #4 pmid=16753168 #5 pmid=15314027 #6 pmid=15489286 #7 pmid=12350376 #8 pmid=10652494 #9 pmid=111634...')
  • 16:02, 6 June 2009 (hist) (diff) Yu:Publications
  • 15:59, 6 June 2009 (hist) (diff) m Yu:Lab Members(templating)
  • 15:37, 6 June 2009 (hist) (diff) m Template:Yu
  • 15:35, 6 June 2009 (hist) (diff) m Yu:Procedures
  • 15:32, 6 June 2009 (hist) (diff) m Yu:YEPD
  • 15:27, 6 June 2009 (hist) (diff) N Yu:YEPD(example soln)
  • 15:15, 6 June 2009 (hist) (diff) m Yu:Procedures(layout)
  • 15:10, 6 June 2009 (hist) (diff) m Template:Yu
  • 23:23, 5 June 2009 (hist) (diff) m Template:Yu
  • 23:19, 5 June 2009 (hist) (diff) Yu:Procedures(generalizing layout)
  • 22:35, 5 June 2009 (hist) (diff) N Yu:Western(New page: =Western Blotting= {{Template:Yu}} __NOTOC__ ===Cell Lysis=== ===SDS PAGE=== ===Transfer=== ===Immunoblotting=== ===Developing===)
  • 19:57, 5 June 2009 (hist) (diff) N User talk:Christopher Jackson(top)
  • 19:57, 5 June 2009 (hist) (diff) N User:Christopher Jackson(top)

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