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• 13:05, 14 August 2009 (hist) (diff) NanoBio: Isolation of Periplasmic Fraction‎ (→Isolation of Periplasmic Fraction) (top)
• 17:15, 10 August 2009 (hist) (diff) NanoBio: Isolation of Periplasmic Fraction‎ (→Spectral measurements of c-cytochromes)
• 17:15, 10 August 2009 (hist) (diff) NanoBio: Isolation of Periplasmic Fraction‎ (→Isolation of Periplasmic Fraction)
• 17:14, 10 August 2009 (hist) (diff) NanoBio: Isolation of Periplasmic Fraction‎ (→Isolation of Periplasmic Fraction)
• 16:52, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Protocol) (top)
• 16:51, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Introduction)
• 16:51, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Protocol)
• 16:50, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Protocol)
• 16:46, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Protocol)
• 16:44, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Protocol)
• 16:32, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Protocol)
• 16:17, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Primer Design)
• 16:03, 10 August 2009 (hist) (diff) NanoBio: Knockout/in‎ (→Knockout/in)
• 15:57, 10 August 2009 (hist) (diff) N NanoBio: Knockout/in‎ (New page: ==Knockout/in== For an example of this, see Heather's notebook C028 (notebook C pg28) The best way to do a knockin is to make the linear fragment of DNA for integration into the genome w...)
• 15:55, 10 August 2009 (hist) (diff) N Image:SOE PCR.jpg‎ (top)
• 15:42, 10 August 2009 (hist) (diff) NanoBio:Protocols‎ (→Gene deletion with λ-red)
• 15:40, 10 August 2009 (hist) (diff) NanoBio: AgaroseGels‎ (→Imaging your gel) (top)
• 15:40, 10 August 2009 (hist) (diff) NanoBio: AgaroseGels‎ (→Ingredients)
• 15:39, 10 August 2009 (hist) (diff) NanoBio: AgaroseGels‎ (→Imaging your gel)
• 13:09, 10 August 2009 (hist) (diff) NanoBio: AgaroseGels‎ (→Procedure)
• 12:52, 10 August 2009 (hist) (diff) NanoBio: AgaroseGels‎ (→Procedure)
• 01:47, 10 August 2009 (hist) (diff) N NanoBio: Isolation of Periplasmic Fraction‎ (New page: ==Isolation of Periplasmic Fraction== Protocol courtesy of Yuri Londer, NEB Make sure these chemicals are available: # # # # # Cell growth # Fractionation # *'''[[User:Heather M. Jens...)
• 01:44, 10 August 2009 (hist) (diff) NanoBio:Protocols‎ (→Protein Expression & Purification)
• 01:42, 10 August 2009 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain) (top)
• 14:21, 15 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎ (→Large-Scale prep) (top)
• 14:17, 15 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎ (→Large-Scale prep)
• 19:41, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→. Day 1)
• 19:41, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Day 1)
• 18:47, 12 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎ (→Small-scale prep)
• 18:47, 12 June 2009 (hist) (diff) NanoBio: Bacterial Transformation using Electroporation‎ (→Simultaneous Transformation using Electroporation) (top)
• 18:46, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Colony PCR for scar)
• 18:44, 12 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎ (→Large-Scale prep)
• 18:29, 12 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎ (→Small-scale prep)
• 18:27, 12 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎
• 18:26, 12 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎ (→Large-scale prep)
• 18:26, 12 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎ (→Small-scale prep)
• 18:25, 12 June 2009 (hist) (diff) NanoBio: Prep for Electrocompetent cells‎ (→Small-scale prep)
• 18:23, 12 June 2009 (hist) (diff) N NanoBio: Prep for Electrocompetent cells‎ (New page: =Small-scale prep= =Large-scale prep= *'''~~~~''': Return to Protocols.)
• 18:22, 12 June 2009 (hist) (diff) NanoBio:Protocols‎ (→Sub-cloning)
• 18:21, 12 June 2009 (hist) (diff) NanoBio: Bacterial Transformation using Electroporation‎ (→Simultaneous Transformation using Electroporation)
• 18:17, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Colony PCR for scar)
• 18:17, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Colony PCR for scar)
• 18:13, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Verify deletion with colony PCR)
• 18:12, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 18:11, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Glycerol stocks to keep)
• 18:11, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 16:31, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 16:30, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 16:30, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 16:29, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Electroporate linear PCR product into electrocompetent cells)
• 16:29, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Electroporate linear PCR product into electrocompetent cells)
• 16:28, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Make {target strain, pKD46} electrocompetent)
• 16:28, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Make {target strain, pKD46} electrocompetent)
• 16:26, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→B. PCR amplify linear fragment from pKD3 or pKD4)
• 16:11, 11 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 16:08, 11 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 16:07, 11 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 15:10, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 15:09, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 15:09, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Make {target strain, pKD46} electrocompetent)
• 15:08, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR amplify linear fragment from pKD3 or pKD4)
• 15:08, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Day 1.A. Transform pKD46 into (chemically competent) target strain)
• 15:06, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Transform pKD46 into (chemically competent) target strain)
• 15:05, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Transform pKD46 into (chemically competent) target strain)
• 15:03, 10 June 2009 (hist) (diff) NanoBio: PCR‎ (→Colony PCR)
• 15:03, 10 June 2009 (hist) (diff) NanoBio: PCR‎ (→PCR verify deletion)
• 15:00, 10 June 2009 (hist) (diff) NanoBio: PCR‎ (→D. Gel analysis and purification of PCR products)
• 16:23, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Set 1) (top)
• 16:22, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Set 2)
• 16:20, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Set 1)
• 16:19, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Set 1)
• 16:11, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Set 1)
• 16:06, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Set 1)
• 16:06, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Set 1)
• 15:31, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Set 1)
• 14:52, 9 June 2009 (hist) (diff) NanoBio: Primer Design‎ (→Primer Design)
• 14:52, 9 June 2009 (hist) (diff) N NanoBio: Primer Design‎ (New page: ==Primer Design== You will need 4 primers per knockout that you design. *2 will be for the PCR to clone out the antibiotic resistance. These are 70 bases long. *The other 2 are for verify...)
• 14:48, 9 June 2009 (hist) (diff) NanoBio: Overview and Materials for λ-red‎ (→Basic outline of procedure) (top)
• 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR amplify linear fragment from pKD3 or pKD4)
• 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Make {target strain, pKD46} electrocompetent)
• 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Electroporate linear PCR product into electrocompetent cells)
• 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Electroporate linear PCR product into electrocompetent cells)
• 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 14:46, 9 June 2009 (hist) (diff) N NanoBio: Overview and Materials for λ-red‎ (New page: ==Overview== This is λ red gene-knockout protocol that our lab uses. It is adapted from the Datsenko and Wanner paper (PNAS, 2000). The goal of this protocol is to use PCR and a plasmid ...)
• 14:36, 9 June 2009 (hist) (diff) NanoBio:Protocols‎ (→Gene deletion with λ-red)
• 14:35, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 14:35, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR amplify linear fragment from pKD3 or pKD4)
• 14:34, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Electroporate linear DNA into electrocompetent cells)
• 14:33, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 14:33, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 13:51, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Remove antibiotic resistance with pCP20)
• 13:35, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 13:33, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 19:46, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 19:45, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 19:45, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 19:41, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR verify deletion)
• 17:55, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Electroporate linear DNA into electrocompetent cells)
• 17:48, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Make {target strain, pKD46} electrocompetent)
• 17:48, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR amplify linear fragment from pKD3 or pKD4)
• 17:44, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→PCR amplify linear fragment from pKD3 or pKD4)
• 17:23, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Transform pKD46 into (chemically competent) target strain)
• 17:22, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout‎ (→Transform pKD46 into (chemically competent) target strain)
• 20:43, 4 June 2009 (hist) (diff) N NanoBio: Protocol for gene knockout‎ (New page: ==Transform pKD46 into (chemically competent) target strain== # Follow instructions from the wiki for transformation of chemically competent cells # grow cells on LB+Amp plates o/n at 30d...)
• 20:29, 4 June 2009 (hist) (diff) NanoBio:Protocols‎ (→Nano Synth Bio)
• 19:24, 4 June 2009 (hist) (diff) N NanoBio: Bacterial Transformation using Electroporation‎ (New page: ==Simultaneous Transformation using Electroporation== Note: this protocol may be used for any cell line, but may have to be optimized. ''There are a couple of tricks to making cells elec...)
• 18:51, 4 June 2009 (hist) (diff) NanoBio:Protocols‎ (→Sub-cloning)
• 18:50, 4 June 2009 (hist) (diff) NanoBio:Protocols‎ (→Sub-cloning)
• 18:49, 4 June 2009 (hist) (diff) NanoBio: Bacterial Transformation‎ (→Simultaneous Transformation)
• 18:49, 4 June 2009 (hist) (diff) NanoBio: Bacterial Transformation‎ (→Traditional Transformation)
• 21:44, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain)
• 21:43, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain)
• 21:42, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain)
• 21:42, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain)
• 21:41, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain)
• 21:41, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain)
• 21:41, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain)
• 21:40, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Heme Stain)
• 21:37, 25 August 2008 (hist) (diff) NanoBio: Protein Gels‎ (→Protein Gels)
• 18:34, 31 July 2008 (hist) (diff) NanoBio: BG-11 media‎ (→BG-11 plates) (top)
• 18:02, 29 July 2008 (hist) (diff) NanoBio: Supplemented M9 Media‎ (→M9 media) (top)
• 18:01, 29 July 2008 (hist) (diff) NanoBio: Supplemented M9 Media‎ (→M9 media)
• 18:00, 29 July 2008 (hist) (diff) NanoBio: Supplemented M9 Media‎ (→M9 media)
• 18:00, 29 July 2008 (hist) (diff) NanoBio: Supplemented M9 Media‎ (→M9 media)
• 17:56, 29 July 2008 (hist) (diff) NanoBio: Supplemented M9 Media‎
• 17:54, 29 July 2008 (hist) (diff) N NanoBio: Supplemented M9 Media‎ (New page: This recipe makes 1L of media supplemented with *xxx% of selected carbon source *1 mM thiamine hydrochloride *0.2% casamino acids Combine all solutions using sterile technique: *200 mL 5...)
• 17:45, 29 July 2008 (hist) (diff) NanoBio:Protocols‎ (→Recipes)
• 21:09, 11 June 2008 (hist) (diff) NanoBio: Bacterial Transformation‎ (→Simultaneous Transformation)
• 21:07, 11 June 2008 (hist) (diff) NanoBio: LB plates‎ (→In a rush?) (top)
• 18:52, 11 June 2008 (hist) (diff) NanoBio:Lab Members‎ (→Lab Members) (top)
• 18:51, 11 June 2008 (hist) (diff) NanoBio:Lab Members‎ (→Lab Members)
• 18:50, 11 June 2008 (hist) (diff) NanoBio:Lab Members‎ (→Lab Members)
• 18:49, 11 June 2008 (hist) (diff) NanoBio: LB plates‎ (→In a rush?)
• 18:48, 11 June 2008 (hist) (diff) NanoBio: LB plates‎ (→In a rush?)
• 18:46, 11 June 2008 (hist) (diff) NanoBio: LB plates‎ (→In a rush?)
• 18:46, 11 June 2008 (hist) (diff) NanoBio: LB plates‎
• 14:27, 11 June 2008 (hist) (diff) NanoBio: Bacterial Transformation‎ (→Simultaneous Transformation)
• 14:26, 11 June 2008 (hist) (diff) NanoBio: Bacterial Transformation‎
• 14:02, 11 June 2008 (hist) (diff) NanoBio: Bacterial Transformation‎
• 14:01, 11 June 2008 (hist) (diff) User:Heather M. Jensen‎ (→Contact Info) (top)
• 13:57, 11 June 2008 (hist) (diff) User:Heather M. Jensen‎ (→Education)
• 13:50, 11 June 2008 (hist) (diff) N User talk:Heather M. Jensen‎ (top)
• 13:50, 11 June 2008 (hist) (diff) N User:Heather M. Jensen‎

(Latest | Earliest) View (newer 500) (older 500) (20 | 50 | 100 | 250 | 500)