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- 15:33, 23 October 2007 (hist) (diff) IGEM:Imperial/2007/Wet Lab/Protocols/Prot1.9 (→Equipment)
- 15:28, 23 October 2007 (hist) (diff) IGEM:Imperial/2007/Wet Lab/Protocols/Prot1.9 (→Reagents)
- 15:26, 23 October 2007 (hist) (diff) IGEM:Imperial/2007/Wet Lab/Protocols/Prot1.9 (→Protocols)
- 15:17, 23 October 2007 (hist) (diff) IGEM:Imperial/2007/Wet Lab/Protocols/Prot1.9 (→Protocols)
- 15:13, 23 October 2007 (hist) (diff) IGEM:Imperial/2007/Wet Lab/Protocols/Prot1.9
- 11:51, 23 October 2007 (hist) (diff) N IGEM:Imperial/2007/Wet Lab/Protocols/Prot1.9 (New page: '''Aims:''' *To determine if <font color=blue>''' pT7- GFP ''</font> DNA constructs for Infector Detector expresses ''in vitro'' *We will be testing at the following temperature: 4 °C,...)
- 14:58, 21 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (→Protocols) (top)
- 14:51, 21 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID3.1 (→Day 2) (top)
- 14:43, 21 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID3.1
- 14:43, 21 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID3.1
- 14:42, 21 October 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID3.1 (New page: =Protocols for experiments to determine sensitivity to AHL of the ID= ==Aims== * To determine the response of the pTet-LuxR-pLux-GFP construct, in-vitro, for different concentrations of A...)
- 14:25, 21 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols (→Protocols)
- 13:51, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.1 (→Loading Plate) (top)
- 13:51, 20 October 2007 (hist) (diff) m IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.1 (→Equipments)
- 13:51, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.1 (→Equipments) (top)
- 13:50, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.1 (→Reagents)
- 13:48, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.1 (→Reagents)
- 13:42, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.2 (top)
- 12:52, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.1 (→Reagents)
- 12:52, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2 (→Reagents) (top)
- 12:52, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.1 (→Reagents)
- 12:50, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2 (→Equipment)
- 12:49, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2 (→Equipment)
- 12:48, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2 (→Equipment)
- 12:42, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (→Equipment)
- 12:39, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.2 (→Equipment)
- 12:35, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1 (→Equipment) (top)
- 12:35, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1 (→Equipment)
- 12:34, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1 (→Protocol)
- 12:32, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1 (→Equipment)
- 12:31, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1 (→Equipment)
- 12:28, 20 October 2007 (hist) (diff) m IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1 (→Reagents)
- 12:28, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1 (→Reagents)
- 12:25, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1 (→Reagents)
- 12:24, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1 (→Equipment)
- 12:24, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1 (→Equipment)
- 12:20, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.2 (→Equipment)
- 12:19, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1 (→Equipment)
- 12:16, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1 (Undo revision 159832 by Special:Contributions/Peixuan Pey (User talk:Peixuan Pey))
- 12:16, 20 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1 (→Equipment)
- 14:16, 18 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2
- 21:50, 4 October 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Shopping Lists (→Shopping List) (top)
- 06:23, 28 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Team Name and Logo (→Discussions about a team name and logo)
- 06:21, 28 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Team Name and Logo
- 15:49, 26 September 2007 (hist) (diff) User talk:Vincent Rouilly
- 15:20, 26 September 2007 (hist) (diff) User talk:Vincent Rouilly
- 15:19, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.2
- 15:18, 26 September 2007 (hist) (diff) m AHL Sensitivity: Real Life Applications (→Loading Plate) (top)
- 15:16, 26 September 2007 (hist) (diff) N Image:Working10.png (top)
- 15:11, 26 September 2007 (hist) (diff) AHL Sensitivity: Real Life Applications (→Schematic)
- 15:10, 26 September 2007 (hist) (diff) N AHL Sensitivity: Real Life Applications (New page: '''Aims:''' The system was tested for AHL sensitivity with concentrations in the range of real biofilms, with AHL concentrations ranging from as low as 0.1nM to 50nM. ===Equipments=== *...)
- 15:03, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.1 (→Loading Plate)
- 15:02, 26 September 2007 (hist) (diff) Preliminary AHL Testing (→Loading Plate) (top)
- 15:02, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.1
- 15:01, 26 September 2007 (hist) (diff) Preliminary AHL Testing (→Loading Plate)
- 14:55, 26 September 2007 (hist) (diff) Preliminary AHL Testing (→Loading Plate)
- 14:48, 26 September 2007 (hist) (diff) N Image:Working9.png (top)
- 14:48, 26 September 2007 (hist) (diff) N Preliminary AHL Testing (New page: '''Aims:''' *To make a rough estimate of the sensitivity of the system to a large range of AHL concentrations *To determine approximations of the threshold of response, time of response, l...)
- 14:29, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2
- 14:27, 26 September 2007 (hist) (diff) Initial Testing of DNA Construct In Vitro (→Protocols) (top)
- 14:27, 26 September 2007 (hist) (diff) N Image:Working8.bmp (top)
- 14:11, 26 September 2007 (hist) (diff) Initial Testing of DNA Construct In Vitro (→Protocols)
- 14:09, 26 September 2007 (hist) (diff) Initial Testing of DNA Construct In Vitro (→Protocols)
- 14:03, 26 September 2007 (hist) (diff) N Initial Testing of DNA Construct In Vitro (New page: '''Aims:''' *To determine if <font color=blue>''' pTet - LuxR - pLux - GFP ([http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002]) '''</font> DNA constructs for Infector Detec...)
- 13:41, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1
- 13:41, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (Undo revision 154373 by Special:Contributions/Peixuan Pey (User talk:Peixuan Pey))
- 13:33, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2
- 13:32, 26 September 2007 (hist) (diff) Initial Testing of DNA Construct In Vivo (→Protocol) (top)
- 13:32, 26 September 2007 (hist) (diff) N Image:Working7.png (top)
- 11:08, 26 September 2007 (hist) (diff) Initial Testing of DNA Construct In Vivo (→Protocol)
- 11:04, 26 September 2007 (hist) (diff) Initial Testing of DNA Construct In Vivo (→Protocol)
- 10:55, 26 September 2007 (hist) (diff) Initial Testing of DNA Construct In Vivo (→Protocol)
- 10:40, 26 September 2007 (hist) (diff) N Initial Testing of DNA Construct In Vivo (New page: '''Aims''' *To test to see if the DNA construct from the registry are viable. This is done in vivo. *The constructs are pTet-LuxR-pLux-GFP. ===Day 1=== ====Equipment==== *7ml sterile tube...)
- 10:04, 26 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol (→Infector Detector) (top)
- 10:03, 26 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol (→Infector Detector)
- 09:52, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2
- 09:52, 26 September 2007 (hist) (diff) Operating Temperature Range (Refined Testing) (top)
- 09:51, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.1 (→Schematic)
- 09:51, 26 September 2007 (hist) (diff) Operating Temperature Range (Refined Testing)
- 09:50, 26 September 2007 (hist) (diff) Operating Temperature Range (Refined Testing) (→In the Teaching Lab)
- 09:50, 26 September 2007 (hist) (diff) Operating Temperature Range (Refined Testing) (→In the Teaching Lab)
- 09:49, 26 September 2007 (hist) (diff) Operating Temperature Range (Refined Testing) (→In the Teaching Lab)
- 09:48, 26 September 2007 (hist) (diff) N Image:Working6.png (top)
- 09:47, 26 September 2007 (hist) (diff) Operating Temperature Range (Refined Testing) (→In the Teaching Lab)
- 09:37, 26 September 2007 (hist) (diff) N Operating Temperature Range (Refined Testing) (New page: =Operating Temperature Range (Refined Testing)= '''Aims:''' *To measure the fluorescence over a 6 hour period, for a range of temperatures. *To continue from the temperatures measured befo...)
- 09:35, 26 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.1
- 09:35, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (top)
- 09:34, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (→Schematic)
- 09:33, 26 September 2007 (hist) (diff) N Image:Working5.png (top)
- 07:56, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (→Schematic)
- 07:49, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (→Schematic)
- 07:43, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (→Schematic)
- 06:17, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (→Reagents)
- 06:13, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (→Equipments)
- 06:12, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (→Equipments)
- 06:11, 26 September 2007 (hist) (diff) Operating Temperature Range (Initial Testing) (→Aims:)
- 06:11, 26 September 2007 (hist) (diff) N Operating Temperature Range (Initial Testing) (New page: ===Aims:=== *To determine if construct expresses ''in vitro'' at temperatures of: 10<sup>o</sup>C, 12<sup>o</sup>C, 20<sup>o</sup>C, 25<sup>o</sup>C, 30<sup>o</sup>C, 37<sup>o</sup>C, 45<s...)
- 06:10, 26 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol (→Cell By Date)
- 15:50, 17 September 2007 (hist) (diff) N PPXSandbox(3a) (New page: ==Experiment 1 == '''Aims''': <br> *Determine the DNA concentration and purity. From this determine volume of the DNA needed to add to the in vitro expression systems. *For this experime...) (top)
- 15:48, 17 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.2
- 15:47, 17 September 2007 (hist) (diff) PPXSandbox(3b) (→Protocols) (top)
- 15:47, 17 September 2007 (hist) (diff) PPXSandbox(3b) (→Protocols)
- 15:46, 17 September 2007 (hist) (diff) PPXSandbox(3b) (→Protocols)
- 15:44, 17 September 2007 (hist) (diff) PPXSandbox(3b)
- 15:42, 17 September 2007 (hist) (diff) PPXSandbox(3b) (→Protocols)
- 15:41, 17 September 2007 (hist) (diff) N Image:Working4 (top)
- 15:36, 17 September 2007 (hist) (diff) N PPXSandbox(3b) (New page: '''Aims''': *To determine which constructs for cell by date expresses ''in vitro'' *To test if <font color=blue>'''pTet-GFP ([http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I...)
- 15:25, 17 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vitro (top)
- 15:24, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol
- 15:24, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol
- 15:22, 17 September 2007 (hist) (diff) PPXSandbox(2a) (Removing all content from page) (top)
- 15:21, 17 September 2007 (hist) (diff) N PPXSandbox(2b) (New page: '''Aims''' *To test to see if the DNA constructs from the registry are viable. This is done in vivo. *The constructs are pTet-GFP and pT7-GFP. ===Day 1=== ====Equipment==== *7ml sterile t...) (top)
- 15:21, 17 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vivo (top)
- 15:19, 17 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1
- 15:18, 17 September 2007 (hist) (diff) PPXSandbox(2a) (→Protocol)
- 15:17, 17 September 2007 (hist) (diff) N Image:Working2.png (top)
- 15:13, 17 September 2007 (hist) (diff) PPXSandbox(2a)
- 15:08, 17 September 2007 (hist) (diff) N PPXSandbox(2a) (New page: '''Aims''' *To test to see if the DNA constructs from the registry are viable. This is done in vivo. *The constructs are pTet-GFP, pT7-GFP and pcI-GFP. ===Day 1=== ====Equipment==== *7ml ...)
- 15:07, 17 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vivo
- 15:07, 17 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vivo
- 15:06, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol (→Cell By Date)
- 15:04, 17 September 2007 (hist) (diff) N PPXSandbox(1b) (New page: '''Aims:''' *Through our modeling it has become clear that in order to get the most effective Infecter detector system we will need LuxR to be at steady state. This is a fundamental proble...) (top)
- 15:03, 17 September 2007 (hist) (diff) Purification of LuxR (top)
- 15:02, 17 September 2007 (hist) (diff) N Purification of LuxR (New page: Old version <br> Refined version)
- 15:00, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol
- 14:53, 17 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.3 (→Protocols)
- 14:52, 17 September 2007 (hist) (diff) N Old version (New page: ==Experiment 1== '''Status:''' *Partially completed *More Data Points needed '''Aims:''' *To create and test a series of dilutions of GFP using a purified sample of GFP '''Equipment''' *5...) (top)
- 14:51, 17 September 2007 (hist) (diff) Refined version (→Protocols) (top)
- 14:45, 17 September 2007 (hist) (diff) Refined version (→Protocols)
- 14:41, 17 September 2007 (hist) (diff) N Image:Working1.png (top)
- 14:39, 17 September 2007 (hist) (diff) Refined version (→Protocols)
- 14:16, 17 September 2007 (hist) (diff) Refined version (→Protocols)
- 13:50, 17 September 2007 (hist) (diff) Refined version (→Protocols)
- 13:48, 17 September 2007 (hist) (diff) N Refined version (New page: '''Aims:''' *Experiment 1 **To create a series of dilutions of GFP using a purified sample of GFP *Experiment 2 **To create a calibration curve to determine: [GFP] in in vitro chassis vs ...)
- 13:47, 17 September 2007 (hist) (diff) GFP vs Fluorescence Calibration Curve (top)
- 13:47, 17 September 2007 (hist) (diff) GFP vs Fluorescence Calibration Curve
- 13:46, 17 September 2007 (hist) (diff) GFP vs Fluorescence Calibration Curve
- 13:45, 17 September 2007 (hist) (diff) GFP vs Fluorescence Calibration Curve
- 13:45, 17 September 2007 (hist) (diff) GFP vs Fluorescence Calibration Curve
- 13:45, 17 September 2007 (hist) (diff) N GFP vs Fluorescence Calibration Curve (New page: ===Old version=== Refined version===)
- 13:44, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol (→Protocols)
- 13:38, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol
- 13:36, 17 September 2007 (hist) (diff) N ===Refined version== (New page: '''Aims''': *To obtain an optimum counting time for the GFP fluorescence. **Optimum counting time: Avoid photobleaching, Minimize variability between readings (A test to see what is the o...) (top)
- 13:35, 17 September 2007 (hist) (diff) Fluorometer- Optimum Exposure Time (Replacing page with '===Old version=== <br> ===Refined version==') (top)
- 13:25, 17 September 2007 (hist) (diff) N Fluorometer- Optimum Exposure Time (New page: '''Aims''': *To obtain an optimum counting time for the GFP fluorescence. **Optimum counting time: Avoid photobleaching, Minimize variability between readings (A test to see what is the o...)
- 13:24, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol
- 13:19, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol
- 13:15, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol (→=Edited Protocols)
- 13:06, 17 September 2007 (hist) (diff) User:Peixuan Pey/SandboxProtocol
- 12:59, 17 September 2007 (hist) (diff) N User:Peixuan Pey/SandboxProtocol (New page: == Protocols == Our cool new layout! {| cellpadding="5" style="background:#F5FAFF; border: 1px solid #aabadd; color:Black" align="left" |- style="background:#aabadd; color:Black" align="c...)
- 12:56, 17 September 2007 (hist) (diff) User:Peixuan Pey (top)
- 12:56, 14 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2 (→In the Teaching Lab)
- 12:22, 14 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2 (→Protocol)
- 12:11, 14 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2
- 12:05, 14 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.2 (New page: '''Aims:''' *To measure the fluorescence over a 6 hour period, for a range of temperatures. *To continue from the temperatures measured before, 37oC and 20oC; temperatures 8oC, 15oC, 25oC ...)
- 11:51, 14 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 11:51, 14 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 11:50, 14 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 10:12, 14 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Schematic)
- 09:25, 13 September 2007 (hist) (diff) User:James Chappell/ Sandbox (→Conclusion)
- 09:02, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 2.1
- 08:47, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.2
- 08:47, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.2 (→Results Summary)
- 08:41, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1
- 08:39, 13 September 2007 (hist) (diff) m IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→Discussion)
- 08:39, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→Results update: 21/8/2007)
- 08:38, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→Results update: 21/8/2007)
- 08:38, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→Results update: 17/8/2007)
- 08:37, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→Results)
- 08:37, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→<br><br> [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 '''pTet-GFP'''])
- 08:37, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1
- 08:34, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1
- 08:09, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1
- 08:05, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→<div style="font-size: 140%; color: Darkblue;"><br><br> [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 '''pTet-GFP'''] </div>)
- 07:37, 13 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.2 (New page: '''Aims:''' The system was tested for AHL sensitivity with concentrations in the range of real biofilms, with AHL concentrations ranging from as low as 0.1nM to 50nM. ===Equipments=== *...)
- 07:35, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.1 (→Protocols)
- 07:32, 13 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID2.1 (New page: '''Aims:''' *To make a rough estimate of the sensitivity of the system to a large range of AHL concentrations *To determine approximations of the threshold of response, time of response, l...)
- 07:27, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 07:21, 13 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD2.1 (New page: '''Aims:''' *To determine if construct expresses ''in vitro'' at temperatures of: 10<sup>o</sup>C, 12<sup>o</sup>C, 20<sup>o</sup>C, 25<sup>o</sup>C, 30<sup>o</sup>C, 37<sup>o</sup>C, 45<s...)
- 07:16, 13 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.3 (New page: '''Aims:''' *To solve the evaporation problem of the samples **Prevent evaporation **Reduce evaporation drastically **Calibrate evaporation if it cannot be solved, so that the results wil...)
- 07:11, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 07:07, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Calendar/2007-9-12
- 06:06, 13 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Calendar/2007-9-12 (New page: ==Debrief== The presentation for Professor Kitney was discussed today. ===Presentation for Professor Kitney===)
- 05:41, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.5 (→Purification 2) (top)
- 05:40, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.5 (→Reagents)
- 05:20, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.5 (→Initial Testing for LuxR solubility)
- 05:10, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.5
- 05:08, 13 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.5 (New page: '''Aims:''' *Through our modeling it has become clear that in order to get the most effective Infecter detector system we will need LuxR to be at steady state. This is a fundamental proble...)
- 05:07, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.4 (top)
- 05:07, 13 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.4 (New page: Aims: To determine the degradation rates of GFP with a range of temperatures.)
- 05:02, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.3 (→Protocols)
- 05:02, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.3 (→Reagents)
- 05:01, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.3 (→Reagents)
- 05:01, 13 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.3 (New page: '''Aims:''' *Experiment 1 **To create a series of dilutions of GFP using a purified sample of GFP *Experiment 2 **To create a calibration curve to determine: [GFP] in in vitro chassis vs ...)
- 04:52, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results2 (→Conclusion)
- 04:51, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results1 (→Conclusion) (top)
- 04:51, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results1 (→Conclusion)
- 04:51, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results1 (→Results)
- 04:51, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results1
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- 04:49, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results1 (→Results)
- 04:49, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results1
- 04:34, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results2
- 04:21, 13 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Improve Methodology/Results2
- 13:32, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 13:31, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 12:08, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 12:07, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 12:03, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 12:00, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.2 (→Reagents) (top)
- 11:59, 12 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.2 (New page: '''Aims''': *To determine if the fluorescence measured changes with the volume of sample it is in. *Investigate whether the the total volume of sample has an affect on fluorescence give...)
- 11:59, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 11:45, 12 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/Prot1.1 (New page: '''Aims''': *To obtain an optimum counting time for the GFP fluorescence. **Optimum counting time: Avoid photobleaching, Minimize variability between readings (A test to see what is the o...) (top)
- 11:41, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 11:41, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 11:28, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 11:24, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2
- 11:23, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.2
- 11:16, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (→Protocols)
- 11:13, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2
- 11:10, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (→Experiment 2)
- 11:09, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (→Experiment 2)
- 11:01, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (→Experiment 2)
- 10:08, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (→Experiment 2)
- 10:07, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2
- 10:06, 12 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.2 (New page: '''Aims:''' *To determine which constructs for cell by date expresses ''in vitro'' *To test the pTet and pcI constructs in commercial S30 E.coli cell extract and home made S30 extract *To ...)
- 09:59, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1
- 09:58, 12 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/ID1.1 (New page: '''Aims''' *To test to see if the DNA constructs from the registry are viable. This is done in vivo. *The constructs are pTet-GFP, pT7-GFP and pcI-GFP. ===Day 1=== ====Equipment==== *7ml ...)
- 09:58, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:56, 12 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.2 (New page: '''Aims''': *To determine which constructs for cell by date expresses ''in vitro'' *To test the pTet and pcI constructs in commercial S30 E.coli cell extract and home made S30 extract *To...)
- 09:56, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:52, 12 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Wet Lab/Protocols/CBD1.1 (New page: '''Aims''' *To test to see if the DNA constructs from the registry are viable. This is done in vivo. *The constructs are pTet-GFP, pT7-GFP and pcI-GFP. ===Day 1=== ====Equipment==== *7ml ...)
- 09:51, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:50, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:48, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:46, 12 September 2007 (hist) (diff) User talk:Vincent Rouilly
- 09:45, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:20, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs of ID In Vivo (top)
- 09:19, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs of ID In Vivo (New page: ===Day 1=== ====Equipment==== *7ml sterile tubes x4 *1.5ml Eppendorf tube x1 *Incubator 37<sup>o</sup>C *Gilson Pipettes ====Reagents==== *''E.coli'' BL21; culture containing pTet-LuxR-pLu...)
- 09:19, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:18, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs of CBD In Vitro (New page: '''Aims''': *To determine which constructs for cell by date expresses ''in vitro'' *To test the pTet and pcI constructs in commercial S30 E.coli cell extract and home made S30 extract *To...) (top)
- 09:18, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:17, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vitro (Removing all content from page)
- 09:16, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs of CBD In Vivo (New page: '''Aims''' *To test to see if the DNA constructs from the registry are viable. This is done in vivo. *The constructs are pTet-GFP, pT7-GFP and pcI-GFP. ===Day 1=== ====Equipment==== *7ml ...) (top)
- 09:16, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:16, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vivo (Removing all content from page)
- 09:14, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:13, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:10, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vitro
- 09:09, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs of CELL BY DATE In Vitro (top)
- 09:08, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs In Vitro (New page: '''Aims''': *To determine which constructs for cell by date expresses ''in vitro'' *To test the pTet and pcI constructs in commercial S30 E.coli cell extract and home made S30 extract *To...)
- 09:08, 12 September 2007 (hist) (diff) m IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 09:08, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs for CELL BY DATE In Vitro (New page: '''Aims''': *To determine which constructs for cell by date expresses ''in vitro'' *To test the pTet and pcI constructs in commercial S30 E.coli cell extract and home made S30 extract *To...) (top)
- 09:07, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
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- 09:07, 12 September 2007 (hist) (diff) m Initial Testing for DNA constructs In Vivo
- 09:06, 12 September 2007 (hist) (diff) m IGEM:IMPERIAL/2007/Wet Lab/Protocols
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- 09:04, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs of CELL BY DATE In Vitro
- 09:00, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs of CELL BY DATE In Vitro (New page: '''Aims''': *Experiment 1 **Determine the DNA concentration and purity. From this determine volume of the DNA needed to add to the ''in vitro'' expression systems. *Experiment 2 **To dete...)
- 08:59, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols
- 08:54, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs of CELL BY DATE In Vivo (top)
- 08:53, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs of INFECTOR DETECTOR In Vivo (top)
- 08:51, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs of CELL BY DATE In Vivo
- 08:51, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs of INFECTOR DETECTOR In Vivo (New page: Aims: *To test to see if the DNA constructs from the registry are viable. This is done in vivo. *The constructs are pTet-LuxR-pLux-GFP. ===Day 1=== ====Equipment==== *7ml sterile tubes x4 ...)
- 08:49, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols (→Protocols)
- 08:48, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs of CELL BY DATE In Vivo (New page: '''Aims''' *To test to see if the DNA constructs from the registry work. This is done in vivo. *The constructs are pTet-GFP and pT7-GFP. ===Day 1=== ====Equipment==== *7ml sterile tubes x...)
- 08:48, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols (→Protocols)
- 08:47, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vivo (Removing all content from page)
- 08:47, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols (→Protocols)
- 08:47, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols (→Protocols)
- 08:46, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols (→Protocols)
- 08:45, 12 September 2007 (hist) (diff) Initial Testing for DNA constructs In Vivo (→Protocol)
- 08:40, 12 September 2007 (hist) (diff) N Initial Testing for DNA constructs In Vivo (New page: ==Protocol== * Status - <font>Completed</font> ===Day 1=== ====Equipment==== *7ml sterile tubes x4 *1.5ml Eppendorf tube x1 *Room temperature 25<sup>o</sup>C *Gilson Pipettes ====Reagent...)
- 08:40, 12 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Wet Lab/Protocols (→Protocols)
- 05:39, 7 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Protocols)
- 06:17, 6 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-9-5 (→Testing of different [AHL] on pTet-LuxR-pLux-GFP DNA construct)
- 06:15, 6 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-9-5 (→Construction of pT7-GFP)
- 19:57, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Shopping Lists (→Shopping List)
- 19:50, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Purification of LuxR)
- 19:50, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Purification of LuxR)
- 19:20, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Protocol)
- 18:34, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Protocol)
- 18:05, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Purification 1)
- 17:43, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Purification 1)
- 17:32, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Purification 1)
- 17:29, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Purification 1)
- 17:23, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Purification 1)
- 09:52, 5 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Notes (→Purification 2)
- 21:25, 4 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Calendar/2007-9-4 (New page: =Progress= ==Infector Detector== ===Purification of LuxR=== Two DNA constructs are needed for the purification of LuxR *one containing the His-tag *one over expressing LuxR <br> Currently ...)
- 20:46, 4 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Steps)
- 20:45, 4 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Steps)
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- 09:08, 3 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Quotes
- 09:07, 3 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Steps)
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- 07:21, 3 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Schematic)
- 07:18, 3 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Experiment 1)
- 07:16, 3 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Reagents)
- 07:14, 3 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Experiment 1)
- 06:59, 3 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.2.1 (→Experiment 1)
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- 05:59, 3 September 2007 (hist) (diff) m IGEM:IMPERIAL/2007/Projects/Shopping Lists (→Acknowledgements)
- 20:47, 2 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Calendar/2007-8-29 (→Cell By Date)
- 20:10, 2 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Calendar/2007-8-29 (→Infector Detector)
- 19:55, 2 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Calendar/2007-8-29 (→Debrief)
- 10:51, 1 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Shopping Lists (→Shopping List)
- 10:49, 1 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Shopping Lists (→Shopping List)
- 10:45, 1 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Shopping Lists (→Shopping List)
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- 10:32, 1 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Shopping Lists (→Shopping List)
- 10:26, 1 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Shopping Lists (→Shopping List)
- 09:45, 1 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-31
- 09:40, 1 September 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-31 (→Cell by date - Operating Temperature Range (continue))
- 09:32, 1 September 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Notebook/2007-8-31 (New page: ==Cell by date - Operating Temperature Range (continue)== '''Construct - pTet-GFP [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522]'''<br> '''Temperatures - 37 °C an...)
- 18:56, 30 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-30 (→Cell by date - Operating Temperature Range)
- 18:55, 30 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-30 (→Cell by date - Operating Temperature Range)
- 16:32, 29 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Protocol 3.2 (→Steps)
- 16:17, 29 August 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Notebook/2007-8-29 (New page: ==In Vitro Testing of viability of home-made cell extract and reaction buffer together with the commercially bought cell extract and pre-incubation mix with pTet at 37<sup>o</sup>C== This ...)
- 19:57, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Protocol 3.2 (→Steps)
- 19:35, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Protocol 3.2 (→Reagents)
- 19:33, 28 August 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Experimental Design/Phase1/Protocol 3.2 (New page: ==Equipments== *Fluorometer + PC *1 well plate *Plate sticker lid ==Reagents== *Pyruvate kinase *rNTPs *S30 cell extract (home made) *Reaction buffer (home made) *Commercial S30 cell extra...)
- 19:25, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Phase1 (→'''3.2 Investigate the viability of home-made cell extract and reaction buffer together with the commercially bought cell extract and pre-incubation mix''')
- 19:21, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Phase1 (→'''3.2 Investigate the viability of home-made cell extract and reaction buffer together with the commercially bought cell extract and pre-incubation mix''')
- 19:18, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Phase1 (→'''3.2 Investigate the viability of home-made cell extract and reaction buffer together with the commercially bought cell extract and pre-incubation mix''')
- 19:02, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Phase1 (→'''3.2 Investigate the viability of home-made cell extract and reaction buffer together with the commercially bought cell extract and pre-incubation mix''')
- 18:52, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Phase1 (→'''3.1 Investigate optimum counting time for fluorodetector''')
- 18:45, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Projects/Experimental Design/Phase1 (→'''3.1 Investigate optimum counting time for fluorodetector''')
- 16:28, 28 August 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Notebook/2007-8-28 (New page: ==In vitro Testing of pTet-LuxR-pLux-GFP at room temperature== Testing of the construct was done at a low concentration of AHL 0.1nM to emulate a real biofilm. This experiment tests whethe...)
- 06:11, 28 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.1.1 (→Schematic)
- 06:10, 28 August 2007 (hist) (diff) N Image:OTR3.bmp (top)
- 17:15, 27 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-24
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- 08:38, 23 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Protocol 1.2 (→Reagents)
- 18:24, 22 August 2007 (hist) (diff) User:Lucas A. Hadjilucas
- 18:23, 22 August 2007 (hist) (diff) User:Peixuan Pey
- 11:51, 22 August 2007 (hist) (diff) User:Peixuan Pey
- 19:08, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-22
- 19:02, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-22 (→Protocol for Operating Temperature Range (Initial Testing phase))
- 18:58, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-22
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- 18:00, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→Results summary)
- 12:37, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-21 (→Protocols)
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- 12:24, 21 August 2007 (hist) (diff) N Image:21 08 2007 plate1.PNG (top)
- 12:21, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-21 (→Protocol 2)
- 12:20, 21 August 2007 (hist) (diff) User:James Chappell/ protocol (→GFP Calibration curve) (top)
- 12:01, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-21 (→Results)
- 11:47, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-21
- 11:46, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Notebook/2007-8-21 (→Aims)
- 11:39, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 2.1 (→Conclusions)
- 11:38, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 2.1 (→Experiment 2)
- 11:31, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1 (→Results summary)
- 11:31, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.2 (Removing all content from page)
- 11:30, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.2
- 11:30, 21 August 2007 (hist) (diff) N IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.2 (New page: pT7 was found to not to work. Its fluorescence reading was negligibly higher than a normal cell culture, and hence our conclusion is that the gene construct is faulty. (This was tested by ...)
- 11:20, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.1.1 (→Aims)
- 11:19, 21 August 2007 (hist) (diff) IGEM:IMPERIAL/2007/Experimental Design/Phase2/Protocol 2.1.1 (→Schematic)
- 11:19, 21 August 2007 (hist) (diff) N Image:OTR2.bmp (top)
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