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- 16:29, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Electroporate linear PCR product into electrocompetent cells)
- 16:28, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Make {target strain, pKD46} electrocompetent)
- 16:28, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Make {target strain, pKD46} electrocompetent)
- 16:26, 12 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→B. PCR amplify linear fragment from pKD3 or pKD4)
- 16:11, 11 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Remove antibiotic resistance with pCP20)
- 16:08, 11 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Remove antibiotic resistance with pCP20)
- 16:07, 11 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Remove antibiotic resistance with pCP20)
- 15:10, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Remove antibiotic resistance with pCP20)
- 15:09, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR verify deletion)
- 15:09, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Make {target strain, pKD46} electrocompetent)
- 15:08, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR amplify linear fragment from pKD3 or pKD4)
- 15:08, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Day 1.A. Transform pKD46 into (chemically competent) target strain)
- 15:06, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Transform pKD46 into (chemically competent) target strain)
- 15:05, 10 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Transform pKD46 into (chemically competent) target strain)
- 15:03, 10 June 2009 (hist) (diff) NanoBio: PCR (→Colony PCR)
- 15:03, 10 June 2009 (hist) (diff) NanoBio: PCR (→PCR verify deletion)
- 15:00, 10 June 2009 (hist) (diff) NanoBio: PCR (→D. Gel analysis and purification of PCR products)
- 16:23, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Set 1) (top)
- 16:22, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Set 2)
- 16:20, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Set 1)
- 16:19, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Set 1)
- 16:11, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Set 1)
- 16:06, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Set 1)
- 16:06, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Set 1)
- 15:31, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Set 1)
- 14:52, 9 June 2009 (hist) (diff) NanoBio: Primer Design (→Primer Design)
- 14:52, 9 June 2009 (hist) (diff) N NanoBio: Primer Design (New page: ==Primer Design== You will need 4 primers per knockout that you design. *2 will be for the PCR to clone out the antibiotic resistance. These are 70 bases long. *The other 2 are for verify...)
- 14:48, 9 June 2009 (hist) (diff) NanoBio: Overview and Materials for λ-red (→Basic outline of procedure) (top)
- 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR amplify linear fragment from pKD3 or pKD4)
- 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Make {target strain, pKD46} electrocompetent)
- 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Electroporate linear PCR product into electrocompetent cells)
- 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Electroporate linear PCR product into electrocompetent cells)
- 14:47, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR verify deletion)
- 14:46, 9 June 2009 (hist) (diff) N NanoBio: Overview and Materials for λ-red (New page: ==Overview== This is λ red gene-knockout protocol that our lab uses. It is adapted from the Datsenko and Wanner paper (PNAS, 2000). The goal of this protocol is to use PCR and a plasmid ...)
- 14:36, 9 June 2009 (hist) (diff) NanoBio:Protocols (→Gene deletion with λ-red)
- 14:35, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Remove antibiotic resistance with pCP20)
- 14:35, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR amplify linear fragment from pKD3 or pKD4)
- 14:34, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Electroporate linear DNA into electrocompetent cells)
- 14:33, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Remove antibiotic resistance with pCP20)
- 14:33, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Remove antibiotic resistance with pCP20)
- 13:51, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Remove antibiotic resistance with pCP20)
- 13:35, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR verify deletion)
- 13:33, 9 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR verify deletion)
- 19:46, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR verify deletion)
- 19:45, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR verify deletion)
- 19:45, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR verify deletion)
- 19:41, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR verify deletion)
- 17:55, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Electroporate linear DNA into electrocompetent cells)
- 17:48, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→Make {target strain, pKD46} electrocompetent)
- 17:48, 8 June 2009 (hist) (diff) NanoBio: Protocol for gene knockout (→PCR amplify linear fragment from pKD3 or pKD4)
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