User contributions
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- 10:36, 26 October 2012 (hist) (diff) User:Sean E. Lauber (→Technical)
- 14:40, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping (top)
- 09:38, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:36, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:36, 16 October 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 09:19, 16 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus (top)
- 14:50, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:20, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:18, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:16, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:12, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 11:09, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 10:58, 15 October 2012 (hist) (diff) Sean Lauber:Isotype ELISA against Adenovirus
- 10:58, 15 October 2012 (hist) (diff) N Sean Lauber:Isotype ELISA against Adenovirus (New page: Want to determine what antibody isotypes are directed against a specific antigen (in my case it was adenovirus). Wash buffer (10X): 800 ml dH2O 24.23 g Tris 87.66 g NaCl 3.73 g KC...)
- 10:54, 15 October 2012 (hist) (diff) Sean Lauber:Generating Adenovirus Lysate (top)
- 10:54, 15 October 2012 (hist) (diff) N Sean Lauber:Generating Adenovirus Lysate (New page: 1. Hela cells were plated onto a T150 (make 2 plates) 2. When confluent, determine the concentration of cells from the second vessel 3. Wash the cells 3x with PBS, remove the media 4. Infe...)
- 10:54, 15 October 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 10:36, 15 October 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 14:48, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:21, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:16, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:12, 21 September 2012 (hist) (diff) Sean Lauber:Kras G12D Genotyping
- 14:09, 21 September 2012 (hist) (diff) N Sean Lauber:Kras G12D Genotyping (New page: This protocol makes use of the DirectPCR mouse tail lysis reagent (Viagen, cat 102-T) to obtain template and the hot start Platinum Taq (Invitrogen, cat 10966-026). 1 mm tail ends are co...)
- 12:00, 18 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 13:07, 17 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 11:54, 13 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 12:15, 7 September 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:49, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:36, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:35, 13 August 2012 (hist) (diff) Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer
- 15:35, 13 August 2012 (hist) (diff) N Sean Lauber:Using the Lewis Lung Carcinoma cell Mouse Model for Lung Cancer (New page: For 5 animals: 1. Thaw a single vial of 1,000,000 cells 2. Add to 9 ml of warmed 10% FBS DMEM 3. Spin at 10,000 RPM for 3 min 4. Discard supernatant 5. Resuspend to 10 ml 6. Plate this vol...)
- 12:08, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development (top)
- 11:51, 11 July 2012 (hist) (diff) N Image:ELISA1.JPG (top)
- 11:51, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development
- 11:49, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development
- 11:49, 11 July 2012 (hist) (diff) Sean Lauber:ELISA Development
- 11:49, 11 July 2012 (hist) (diff) N Image:ELISA1.jpg (top)
- 11:45, 11 July 2012 (hist) (diff) N Sean Lauber:ELISA Development (New page: First you need to determine what reagents you will use for your ELISA. The protocol I use is based off of a generic R&D ELISA. The primary antibody binds to the plate (this should be monoc...)
- 11:20, 11 July 2012 (hist) (diff) Sean Lauber:PCR (top)
- 11:18, 11 July 2012 (hist) (diff) N Sean Lauber:PCR (New page: For each reaction: 18.15 μL nuclease free water 2.5 μL 10X PCR buffer 0.5 μL 10 μM dNTPs 0.75 μL 50 mM MgCl2 1 μL 25 μM primer A 1 μL 25 μM primer B 1 μL ...)
- 11:05, 11 July 2012 (hist) (diff) User:Sean E. Lauber (→Molecular Techniques)
- 10:37, 11 April 2012 (hist) (diff) Sean Lauber:Broncho-alveolar Lavage (top)
- 10:37, 11 April 2012 (hist) (diff) N Sean Lauber:Broncho-alveolar Lavage (New page: This procedure explains how to sacrifice the mouse (by bleeding/asphyxiation). Once the mouse is bled and the diaphragm is cut back the mouse is likely dead and the anasthetic nose tube ca...)
- 12:51, 3 April 2012 (hist) (diff) User:Sean E. Lauber (→Protocols)
- 12:51, 3 April 2012 (hist) (diff) Joyce: Preparing RNA from yeast (→Concept) (top)
- 12:49, 3 April 2012 (hist) (diff) Joyce: Extraction of DNA from an agarose gel (→Procedure) (top)
- 12:49, 3 April 2012 (hist) (diff) Joyce: Extraction of DNA from an agarose gel (→Concept)
- 12:46, 3 April 2012 (hist) (diff) N Sean Lauber:Freezing Mammalian Cells (New page: I like to freeze down enough cells to comfortably expand them in a T25. Depending on the cell line this number could vary. I always keep an additional flask with cells to ensure I still ha...) (top)
- 11:53, 3 April 2012 (hist) (diff) User:Sean E. Lauber (→Education & Research Projects)
- 23:08, 2 April 2012 (hist) (diff) Sean Lauber:ImageJ - Threshold Analysis (top)
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