Springer Lab:Microscopes

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objectives)
Current revision (10:13, 25 June 2011) (view source)
 
Line 13: Line 13:
*the Nikon Ti is equipped with a perfect focus unit (http://www.microscopyu.com/articles/livecellimaging/perfectfocus.html)
*the Nikon Ti is equipped with a perfect focus unit (http://www.microscopyu.com/articles/livecellimaging/perfectfocus.html)
*the microscope is controlled by Micro-Manager (http://micro-manager.org/)
*the microscope is controlled by Micro-Manager (http://micro-manager.org/)
-
*control via Matlab is also possible (https://valelab.ucsf.edu/~nico/MMwiki/index.php/Matlab_Configuration)
+
*control via Matlab is also possible (http://valelab.ucsf.edu/~MM/MMwiki/index.php/Matlab)
====Objectives====
====Objectives====

Current revision

Home        Contact        Internal        Lab Members        Publications        Research        Talks       



Contents

Nikon Ti microscope)

Sign-up list

User / Date / Duration

Configuration

General

Objectives

Coming soon...

Cameras

Filter cubes

  • 1. DAPI (Semrock LF405-A: excitation 390/40, dichroic 405, emission 452/45)
  • 2. CFP (excitation 438/24, dichroic 458, emission 483/32)
  • 3. GFP (excitation 472/30, dichroic 495, emission 520/35)
  • 4. YFP (excitation 500/24, dichroic 520, emission 542/27)
  • 5. RFP (excitation 562/40, dichroic 593, emission 641/75), this filter was designed for true RFPs like mCherry
  • 6. Bf/Phase



filters for the lasers

Coming soon....

Lasers

Maintenance log

  • Install new DAPI filter set. A new cfg file was genereated for micromanager.
  • 2011.05.06 Install new versions of micromanager 1.3.46, 1.3.41
  • 2011.05.06 Changed drivers versions from

Ti Control: Ver 1.0.54
Ti SDK: Ver 1.0.78
Eclipse Ti: Ver.V.2.10


To-do list:

  • Matlab problems with PSF. Cannot detect the state of the PSF.
  • The PSF does not engage when moving in several positions of the plate.



Personal tools