Springer Lab:Protocols: Difference between revisions
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==Protocols== | ==Protocols== | ||
===''S. cerevisiae'' Protocols=== | |||
*[http://dbb.urmc.rochester.edu/labs/sherman_f/yeast/6.html Yeast Nomenclature] | |||
*[[Springer Lab: TransformationYeast | Yeast Transformation (Gietz)]] - Our standard yeast transformation protocol. | |||
<!-- *[[Springer Lab: TransformationYeast | Yeast Transformation (Cold Spring Harbor?)]] - a variant of the above protocol that differs in final PEG and LiAc concentrations.--> | |||
<!-- *[[Springer Lab: TransformationYeast2 | Yeast Transformation 2]] - short version of protocol--> | |||
*[[Springer Lab: TransformationYeast96well | Transformation Yeast 96 well]] | |||
*[[Springer Lab: GenomicDNAIsolation | Quick and easy isolation of genomic DNA from Yeast]] | |||
*[[Springer Lab: Sporulation | Sporulation of diploid yeast]] | |||
*[[Springer Lab: Random Spore Analysis | Random Spore Analysis]] | |||
===Recipes=== | |||
Growth media | |||
**[[Springer Lab: | *[[Springer Lab: YNB media|YNB media]] | ||
==Antibiotics== | |||
*[[Springer Lab: Antibiotics|Antibiotics]] | |||
==High Throughput Techniques== | |||
*[[Springer Lab: Handlingplates|Tips on handling plates]] | |||
==Isothermal Assembly== | |||
*[[Springer Lab: Isothermal Assembly|Isothermal Assembly]] | |||
*[[Springer Lab: Isothermal Kits|Isothermal Kits]] | |||
==Flow Cytometry== | |||
*[http://www.isac-net.org/index.php?option=com_content&task=view&id=101&Itemid=150 FCS file format] | |||
==Lab Maintenance== | |||
*[[Springer Lab: CleaningGlassBeads|Cleaning Glass Beads]] | |||
Latest revision as of 05:48, 23 May 2012
Protocols
S. cerevisiae Protocols
- Yeast Nomenclature
- Yeast Transformation (Gietz) - Our standard yeast transformation protocol.
- Transformation Yeast 96 well
- Quick and easy isolation of genomic DNA from Yeast
- Sporulation of diploid yeast
- Random Spore Analysis
Recipes
Growth media
