Springer Lab:Protocols: Difference between revisions
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*[http://dbb.urmc.rochester.edu/labs/sherman_f/yeast/6.html Yeast Nomenclature] | *[http://dbb.urmc.rochester.edu/labs/sherman_f/yeast/6.html Yeast Nomenclature] | ||
*[[Springer Lab: TransformationYeast | Yeast Transformation (Gietz)]] - Our standard yeast transformation protocol. | *[[Springer Lab: TransformationYeast | Yeast Transformation (Gietz)]] - Our standard yeast transformation protocol. | ||
*[[Springer Lab: TransformationYeast | Yeast Transformation (Cold Spring Harbor?)]] - a variant of the above protocol that differs in final PEG and LiAc concentrations. | <!-- *[[Springer Lab: TransformationYeast | Yeast Transformation (Cold Spring Harbor?)]] - a variant of the above protocol that differs in final PEG and LiAc concentrations.--> | ||
*[[Springer Lab: TransformationYeast2 | Yeast Transformation 2]] - short version of protocol | <!-- *[[Springer Lab: TransformationYeast2 | Yeast Transformation 2]] - short version of protocol--> | ||
*[[Springer Lab: TransformationYeast96well | Transformation Yeast 96 well]] | *[[Springer Lab: TransformationYeast96well | Transformation Yeast 96 well]] | ||
*[[Springer Lab: GenomicDNAIsolation | Quick and easy isolation of genomic DNA from Yeast]] | *[[Springer Lab: GenomicDNAIsolation | Quick and easy isolation of genomic DNA from Yeast]] | ||
*[[Springer Lab: Sporulation | Sporulation of diploid yeast]] | |||
*[[Springer Lab: Random Spore Analysis | Random Spore Analysis]] | |||
===Recipes=== | ===Recipes=== | ||
Growth media | Growth media | ||
*[[Springer Lab: YNB media|YNB media]] | *[[Springer Lab: YNB media|YNB media]] | ||
==Antibiotics== | ==Antibiotics== | ||
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*[[Springer Lab: Isothermal Assembly|Isothermal Assembly]] | *[[Springer Lab: Isothermal Assembly|Isothermal Assembly]] | ||
*[[Springer Lab: Isothermal Kits|Isothermal Kits]] | *[[Springer Lab: Isothermal Kits|Isothermal Kits]] | ||
==Flow Cytometry== | |||
*[http://www.isac-net.org/index.php?option=com_content&task=view&id=101&Itemid=150 FCS file format] | |||
==Lab Maintenance== | ==Lab Maintenance== | ||
*[[Springer Lab: CleaningGlassBeads|Cleaning Glass Beads]] | *[[Springer Lab: CleaningGlassBeads|Cleaning Glass Beads]] | ||
Latest revision as of 05:48, 23 May 2012
Protocols
S. cerevisiae Protocols
- Yeast Nomenclature
- Yeast Transformation (Gietz) - Our standard yeast transformation protocol.
- Transformation Yeast 96 well
- Quick and easy isolation of genomic DNA from Yeast
- Sporulation of diploid yeast
- Random Spore Analysis
Recipes
Growth media
