Springer Lab:Protocols

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Protocols

S. cerevisiae Protocols

• Yeast Nomenclature
• Yeast Transformation (Gietz) - Our standard yeast transformation protocol.
• Yeast Transformation (Cold Spring Harbor?) - a variant of the above protocol that differs in final PEG and LiAc concentrations.
• Yeast Transformation 2 - short version of protocol
• Transformation Yeast 96 well
• Quick and easy isolation of genomic DNA from Yeast

Recipes

Growth media

• YNB media

For yeast transformations

• 50% PEG

Antibiotics

• Antibiotics

High Throughput Techniques

• Tips on handling plates

Isothermal Assembly

• Isothermal Assembly
• Isothermal Kits

Flow Cytometry

• FCS file format

Lab Maintenance

• Cleaning Glass Beads