Stacie Stone

Stacie Stone, Ph.D.

Stacie received her Ph.D. in Human Genetics at the Vrije University, Amsterdam, The Netherlands in 2008 after earning her B.S. in Molecular Biology/Biotechnology from Portland State University in 2000. She arrived at OHSU in October of 1998.

Research Interest: Molecular Mechanism of Human Cancer Susceptibility

The Fanconi Anemia pathway

‘Caretaker’ type gene diseases, such as Fanconi Anemia (FA), provide an opportunity to understand the fundamental mechanisms of host susceptibility to cancer. Unlike many caretaker gene diseases, where the defects have been associated with specific molecular processes, the precise underlying molecular defect in FA is unknown.

Although recent developments in the FA field have furthered our understanding of the FA pathway and its role in genomic stability, there are still unidentified FA patient groups, which means there are still unidentified FA genes. Until all the FA proteins, their complexes, and other important functional cofactors that impact the FA pathway are identified we are missing necessary pieces to complete the FA pathway puzzle.

• Current focus: To identify functionally important proteins and protein complexes within the FA pathway.

• Long-term goal: Identify functionally important proteins within the FA pathway to aid in the development of cell-free screens for small molecules that could translate into therapeutics for FA patients.

Publications

1. Igor Landais*, Alexandra Sobeck*, Stacie Stone*, Alexis LaChapelle, Maureen E. Hoatlin. A novel cell-free screen identifies a potent inhibitor of the Fanconi anemia pathway. International Journal of Cancer 2008 (In press) PDF Preprint
2. Lily Chien Wang, Stacie Stone, Maureen E Hoatlin and Jean Gautier. Fanconi anemia proteins stabilize replication forks. DNA repair 2008 (In press) PDF Preprint
3. Stone, S., Sobeck, A., van Kogelenberg M., de Graaf B., Joenje H., Christian J., and Hoatlin ME. Identification, Developmental Expression, and Regulation of the Xenopus Ortholog of Human FANCG/XRCC9. Genes to Cells 2007 Jul;12(7):841–851. abstract
4. Sobeck, A, Stone, S., Hoatlin, ME. DNA Structure-Induced Recruitment and Activation of the Fanconi Anemia Pathway Protein, FANCD2. 2007 Jun;27(12):4283-92. abstract
5. Stone, S.*, Sobeck, A.*, Costanzo, V., de Graaf, B., Reuter, T. de Winter, J., Wallisch, M., Akkari, Y., Olson, S., Wang, W., Joenje, H., Christian, J., Lupardus, P. L., Cimprich, K.A., Gautier, J., and Hoatlin, M. E. Fanconi Anemia Proteins are Required to Prevent Accumulation of Replication-Associated DNA Double Strand Breaks. Mol Cell Biol. 2006 Jan;26(2):425-37. abstract PDF reprint
6. Meetei AR, Medhurst AL, Ling C, Xue Y, Singh TR, Bier P, Steltenpool J, Stone S , Dokal I, Mathew CG, Hoatlin M, Joenje H, de Winter JP, Wang W. A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M. Nat Genet. 2005, 37(9):958-63. abstract
7. Dai MS, Chevallier N, Stone S, Heinrich MC, McConnell M, Reuter T, Broxmeyer HE, Licht JD, Lu L, Hoatlin ME. The effects of the Fanconi anemia zinc finger (FAZF) on cell cycle, apoptosis, and proliferation are differentiation stage-specific. J Biol Chem. 2002 Jul 19;277(29):26327-34. abstract
8. van de Vrugt HJ, Koomen M, Berns MA, de Vries Y, Rooimans MA, van der Weel L, Blom E, de Groot J, Schepers RJ, Stone S, Hoatlin ME, Cheng NC, Joenje H, Arwert F. Characterization, expression and complex formation of the murine Fanconi anaemia gene product Fancg. Genes Cells. 2002 Mar;7(3):333-42. abstract
9. de Winter JP, van der Weel L, de Groot J, Stone S, Waisfisz Q, Arwert F, Scheper RJ, Kruyt FA, Hoatlin ME, Joenje H. The Fanconi anemia protein FANCF forms a nuclear complex with FANCA, FANCC and FANCG. Hum Mol Genet. 2000 Nov 1;9(18):2665-74. abstract
10. Heinrich MC, Silvey KV, Stone S, Zigler AJ, Griffith DJ, Montalto M, Chai L, Zhi Y, Hoatlin ME. Posttranscriptional cell cycle-dependent regulation of human FANCC expression. Blood. 2000 Jun 15;95(12):3970-7. abstract
11. Hoatlin ME, Zhi Y, Ball H, Silvey K, Melnick A, Stone S, Arai S, Hawe N, Owen G, Zelent A, Licht JD.A novel BTB/POZ transcriptional repressor protein interacts with the Fanconi anemia group C protein and PLZF. Blood. 1999 Dec 1;94(11):3737-47. abstract

Abstracts/Talks

FA Research Symposium, Houston, Texas 2003

• ASH conference, San Francisco 2008

• FA Research Symposium, Chicago, Illinois 2007

• FA Research Symposium, Bethesda, Maryland 2006

• FA Research Symposium, Geneva, Switzerland 2005

• FA Research Symposium, Cambridge, Massachusetts 2004

• Ventura, California 2003

• FA Research Symposium, Houston, Texas 2003

• Ash Conference 2003

• FA Research Symposium, Portland, Oregon 2001

Visiting Scholar

• 2008 Visiting Scientist, University of California, Berkeley, California Laboratory of Dr. Rebecca Heald

• 2005 Visiting Scientist, National Institute of Health, Baltimore, Maryland Laboratory of Dr. Weidong Wang

• 2003 Visiting Scientist, Free University of Amsterdam, The Netherlands Laboratory of Dr. Hans Joenje

Education

• 2003- April 24, 2008 Ph.D. candidate: Department of Clinical Genetics and Human Genetics, VU University Medical Center, Amsterdam, The Netherlands (under the supervision of Dr. Maureen Hoatlin and Dr. Hans Joenje)

• B.S. in Molecular Biology and Biotechnology, Portland State University 2000

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