Standard E. coli Strain for BioBricks: Difference between revisions
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*Knockout natural lac... | *Knockout natural lac... | ||
*etc | *etc | ||
A paper that I ran across - "Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway.", by Flores et al. | |||
Short version - they overexpressed G6PDH and largely restored the growth rate of a strain carrying a high copy plasmid. Doesn't solve the problem of protein load, but it might be useful in a BB strain, particularly for constructing (non-functional or non-induced) intermediates. -- Josh | |||
[[Category:Project]] | [[Category:Project]] | ||
Revision as of 08:15, 2 June 2005
This project intends to build a standard strain for use with BioBricks.
We will be making a series of mutations from wildtype K12 E.Coli.
- Knockout natural lac...
- etc
A paper that I ran across - "Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway.", by Flores et al. Short version - they overexpressed G6PDH and largely restored the growth rate of a strain carrying a high copy plasmid. Doesn't solve the problem of protein load, but it might be useful in a BB strain, particularly for constructing (non-functional or non-induced) intermediates. -- Josh
