Standard E. coli Strain for BioBricks

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Revision as of 11:15, 2 June 2005 by Josh K. Michener (Talk | contribs)
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This project intends to build a standard strain for use with BioBricks.

We will be making a series of mutations from wildtype K12 E.Coli.

  • Knockout natural lac...
  • etc

A paper that I ran across - "Growth-rate recovery of Escherichia coli cultures carrying a multicopy plasmid, by engineering of the pentose-phosphate pathway.", by Flores et al. Short version - they overexpressed G6PDH and largely restored the growth rate of a strain carrying a high copy plasmid. Doesn't solve the problem of protein load, but it might be useful in a BB strain, particularly for constructing (non-functional or non-induced) intermediates. -- Josh

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