Stanford/BIOE44:Module3:Day2:atrazine

STATUS

Atrazine Sensor Project

Authors: Frank Li, Claire Durkin, and Sanjay Saraf

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1. reverse complement of atzR (969 bp) (bp #99885..100853 on Psuedomonas sp. ADP http://www.ncbi.nlm.nih.gov/nuccore/13937422)

gaattcgcggccgcttctagagtcacgttgcattgtgggtcgtatcagcgagcttgccggcgacgaactccgcaaatgccgccgatgccacaggcagcaccctctcacgcaatgcgaccagtacga cgcgtccggaagccaggctgcgctcgtcgattggaattgcaacctcaccctccgtggaaccagcaccgatctcgatctgaaagctcaccccaccggtttcgcgggcaaaaccgcgcatcatttcgt aggaattactgaccagttgaggccgcggtcgagctgatgatttgtcgaataactcgtccaacacgttgcgcccggagagactgctgtccggtagcgcaagggggtagtccaggcaatctttcagac gcagactcgtacgcttcgcgagcggatggtcggaagcaacgacggcacagatgcgttgccgcgcttgtgcaatgacagtcaaaccgcgcaggtccgggggattgaagaccagcgccaggtcggccg cgaaatccgtcacggcgagaacagcacgctcgcggtccagcacctttacgtcgaaggcaatacctggacgttgggcctggaacgcgtgtattacgcggggcaggaactccggagccagcgcctggc tggctgccaatgtcacacggccgcgtttcatgccgctgagatcctggatctgtgactggacttgttccagatcggcattgcggcgacgagcgtaggcaacgaacagttcgcctgccgcagtcaggc gtacaccacgcggcagacgctcgaaaatcggcgtacctaactcgtattccagatcctggacgcggcgatttaccgctgatgcggcgacgtgcagctgttctgcggcggcccgaatggagccgcagc gagccacggcatcgatgtaatgaagaaagcgtaaatgttgcataggtggtcgaagtaaaaaggatggaatagagatggcctgtatcgctgacggccgctgtgcccgcattactagtagcggccgct gcag

EcoRI (3), NotI (1) removed

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2. reverse complement of constitutive promoter J23101 (35bp) (from http://partsregistry.org/Part:BBa_J23101:Design)

gaattcgcggccgcttctagaggctagcataatacctaggactgagctagctgtaaatactagtagcggccgctgcag

-no enzyme sites

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3. atzDEF operator region (96 bp) (bp #100851..100946 on Psuedomonas sp. ADP http://www.ncbi.nlm.nih.gov/nuccore/13937422)

Sequence: gaattcgcggccgcttctagagcatgcgagtcaaagcaagatcggtgccggatcggcaccagttaggtcggaaaaaggcggcagtcaagtgcgcagggcggcgttaagcttgaacgaatactagta gcggccgctgcag

Long Description: The atzR, atzDEF operon region is the regulatory region for the atzR gene and the atzDEF genes. This operon is regulated by AtzR, which binds to the atzR promoter and atzDEF promoter, blocking/repressing it. The presence of cyanuric acid “frees” AtzR from being bound to the atzDEF promoter so RNA polymerase can now bind and transcribe following gene. AtzR remains bound to the atzR promoter region even in the presence of cyanuric acid. No enzyme sites were altered in making the standardized BioBrick part.

Short Description: Coding sequence for the atzR, atzDEF operon region. Regulated by the binding of AtzR protein.

Sequence Information: Link to [1]

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4. glnK from Psuedomonas putida KT2440 (339 bp) (bp #5967730..5968070 on Psuedomonas putida KT2440 http://www.ncbi.nlm.nih.gov/nuccore/NC_009512)

gaattcgcggccgcttctagatgaagctagtcacagccatcatcaagccgttcaagctggacgacgtgcgcgagtcgctgtcggaaatcggcgtgcagggcatcaccgtcaccgaagtcaaaggttt cggtcggcagaagggccacaccgagctgtatcgcggtgctgaatatgtggtcgatttcctgcccaaggtgaagatcgatgtcgccatcgatgacaaagaccttgatcgggtaatcgaagccatcacc aaggcagccaacaccggcaagatcggtgacggcaagattttcgtggtgaatctggagcaggcgatccgcatccgtaccggcgaaaccgataccgacgcgatctaatactagtagcggccgctgcag

-no enzyme sites