Stanford/BIOE44:Module3:Day2:nitrate II: Difference between revisions
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Long Description: We plan to develop a system that can sense a low level of nitrate in water by using a mutant of nitrate-inducible fnr coupled with the narG promoter. While normally only inducible under anaerobic conditions, a single silent mutation to convert leucine-28 to histidine can allow the fnr gene to operate under anaerobic conditions. The fnr gene acts as a promoter for narG, which can send a PoPs-out signal to a GFP reporter. Previous studies have shown that the mutated form of fnr can sense nitrate at as low as 0.1 uM. | Long Description: We plan to develop a system that can sense a low level of nitrate in water by using a mutant of nitrate-inducible fnr coupled with the narG promoter. While normally only inducible under anaerobic conditions, a single silent mutation to convert leucine-28 to histidine can allow the fnr gene to operate under anaerobic conditions. The fnr gene acts as a promoter for narG, which can send a PoPs-out signal to a GFP reporter. Previous studies have shown that the mutated form of fnr can sense nitrate at as low as 0.1 uM. | ||
BBa_narGp001 sequence: | ''BBa_narGp001 sequence:'' | ||
GAATTC GCGGCCGC T TCTAGA G | GAATTC GCGGCCGC T TCTAGA G ATCCTAAAGGGGTATCTTAGGAATTTACTTTATTTTTCATCCCCATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCAC T ACTAGT A GCGGCCG CTGCAG | ||
ATCCTAAAGGGGTATCTTAGGAATTTACTTTATTTTTCATCCCCATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCAC | |||
T ACTAGT A GCGGCCG CTGCAG | |||
The promoter itself does not contain any BioBrick restriction enzyme sites, so this part is in accordance with BioBrick Standard 10. | The promoter itself does not contain any BioBrick restriction enzyme sites, so this part is in accordance with BioBrick Standard 10. | ||
Revision as of 15:15, 4 May 2010
Nitrate Sensor Project
Authors: Daniel Bui and Aaditya Shidham
Created BioBrick Parts: BBa_fnr001, BBa_narGp001
Long Description: We plan to develop a system that can sense a low level of nitrate in water by using a mutant of nitrate-inducible fnr coupled with the narG promoter. While normally only inducible under anaerobic conditions, a single silent mutation to convert leucine-28 to histidine can allow the fnr gene to operate under anaerobic conditions. The fnr gene acts as a promoter for narG, which can send a PoPs-out signal to a GFP reporter. Previous studies have shown that the mutated form of fnr can sense nitrate at as low as 0.1 uM.
BBa_narGp001 sequence:
GAATTC GCGGCCGC T TCTAGA G ATCCTAAAGGGGTATCTTAGGAATTTACTTTATTTTTCATCCCCATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCAATGTCGATTTATCAGAGGGCCGACAGGCTCCCAC T ACTAGT A GCGGCCG CTGCAG
The promoter itself does not contain any BioBrick restriction enzyme sites, so this part is in accordance with BioBrick Standard 10.
