Stanford/BIOE44:Module3:Day2:nitrate II: Difference between revisions
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The promoter itself does not contain any BioBrick restriction enzyme sites, so this part is in accordance with BioBrick Standard 10. | The promoter itself does not contain any BioBrick restriction enzyme sites, so this part is in accordance with BioBrick Standard 10. | ||
fnr operator/promoter sequence: | |||
GAATTC GCGGCCGC T TCTAGAG | |||
TATATTTACATCTAATATCGAAATTCTCTGCTGTTAAGGTTTGCTTAGACTTAC | |||
TTGCTCCCTAAAAAGATGTTAAAATTGACAAATATCAATTACGGCTT | |||
T ACTAGT A GCGGCCG CTGCAG | |||
fnr ORF sequence: | |||
GAATTC GCGGCCGC T TCTAG | |||
ATGATCCCGGAAAAGCGAATTATACGGCGCATTCAGTCTGGCGGTTGTGCTATCCATTGCCAGGATTGC | |||
AGCATCAGCCAGCATTGCATCCCGTTCACACTCAACGAACATGAGCTTGATCAGCTTGATAATATCATT | |||
GAGCGGAAGAAGCCTATTCAGAAAGGCCAGACGCTGTTTAAGGCTGGTGATGAACTTAAATCGCTTTAT | |||
GCCATCCGCTCCGGTACGATTAAAAGTTATACCATCACTGAGCAAGGCGACGAGCAAATCACTGGTTTC | |||
CATTTAGCAGGCGACCTGGTGGGATTTGACGCCATCGGCAGCGGCCATCACCCGAGCTTCGCGCAGGCG | |||
CTGGAAACCTCGATGGTATGTGAAATCCCGTTCGAAACGCTGGACGATTTGTCCGGTAAAATGCCGAAT | |||
CTGCGTCAGCAGATGATGCGTCTGATGAGCGGTGAAATCAAAGGCGATCAGGACATGATCCTGCTGTTG | |||
TCGAAGAAAAATGCCGAGGAACGTCTGGCTGCATTCATCTACAACCTGTCCCGTCGTTTTGCCCAACGC | |||
GGCTTCTCCCCTCGTGAGTTCCGCCTGACGATGACTCGTGGCGATATCGGTAACTATCTGGGCCTGACG | |||
GTAGAAACCATCAGCCGTCTGCTGGGTCGCTTCCAGAAAAGCGGCATGCTGGCAGTCAAAGGTAAATAC | |||
ATCACCATCGAAAATAACGATGCGCTGGCCCAGCTTGCTGGTCATACGCGTAACGTTGCCTAATAA | |||
T ACTAGT A GCGGCCG CTGCAG | |||
Revision as of 15:18, 4 May 2010
Nitrate Sensor Project
Authors: Daniel Bui and Aaditya Shidham
Created BioBrick Parts: BBa_fnr001, BBa_narGp001
Long Description: We plan to develop a system that can sense a low level of nitrate in water by using a mutant of nitrate-inducible fnr coupled with the narG promoter. While normally only inducible under anaerobic conditions, a single silent mutation to convert leucine-28 to histidine can allow the fnr gene to operate under anaerobic conditions. The fnr gene acts as a promoter for narG, which can send a PoPs-out signal to a GFP reporter. Previous studies have shown that the mutated form of fnr can sense nitrate at as low as 0.1 uM.
BBa_narGp001 sequence:
GAATTCGCGGCCGCTTCTAGAGATCCTAAAGGGGTATCTTAGGAATTTACTTTATTTTTCATCCCC ATCACTCTTGATCGTTATCAATTCCCACGCTGTTTCAGAGCGTTACCTTGCCCTTAAACATTAGCA ATGTCGATTTATCAGAGGGCCGACAGGCTCCCACTACTAGTAGCGGCCGCTGCAG
The promoter itself does not contain any BioBrick restriction enzyme sites, so this part is in accordance with BioBrick Standard 10.
fnr operator/promoter sequence: GAATTC GCGGCCGC T TCTAGAG TATATTTACATCTAATATCGAAATTCTCTGCTGTTAAGGTTTGCTTAGACTTAC TTGCTCCCTAAAAAGATGTTAAAATTGACAAATATCAATTACGGCTT T ACTAGT A GCGGCCG CTGCAG
fnr ORF sequence: GAATTC GCGGCCGC T TCTAG ATGATCCCGGAAAAGCGAATTATACGGCGCATTCAGTCTGGCGGTTGTGCTATCCATTGCCAGGATTGC AGCATCAGCCAGCATTGCATCCCGTTCACACTCAACGAACATGAGCTTGATCAGCTTGATAATATCATT GAGCGGAAGAAGCCTATTCAGAAAGGCCAGACGCTGTTTAAGGCTGGTGATGAACTTAAATCGCTTTAT GCCATCCGCTCCGGTACGATTAAAAGTTATACCATCACTGAGCAAGGCGACGAGCAAATCACTGGTTTC CATTTAGCAGGCGACCTGGTGGGATTTGACGCCATCGGCAGCGGCCATCACCCGAGCTTCGCGCAGGCG CTGGAAACCTCGATGGTATGTGAAATCCCGTTCGAAACGCTGGACGATTTGTCCGGTAAAATGCCGAAT CTGCGTCAGCAGATGATGCGTCTGATGAGCGGTGAAATCAAAGGCGATCAGGACATGATCCTGCTGTTG TCGAAGAAAAATGCCGAGGAACGTCTGGCTGCATTCATCTACAACCTGTCCCGTCGTTTTGCCCAACGC GGCTTCTCCCCTCGTGAGTTCCGCCTGACGATGACTCGTGGCGATATCGGTAACTATCTGGGCCTGACG GTAGAAACCATCAGCCGTCTGCTGGGTCGCTTCCAGAAAAGCGGCATGCTGGCAGTCAAAGGTAAATAC ATCACCATCGAAAATAACGATGCGCTGGCCCAGCTTGCTGGTCATACGCGTAACGTTGCCTAATAA T ACTAGT A GCGGCCG CTGCAG
