Stanford/BIOE44:Module 1:Day1
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M1: Day 1 - Plasmid Extraction and Restriction Digest
Introduction
Before Class
- Please read:
In Class
Part 1: Plasmid extraction (aka miniprep)
At the beginning of class you will be given a 5mL culture of E. coli harboring a plasmid that contains a BioBrick part. We will extract the plasmid from the bacteria using a kit (QIAPrep Spin Miniprep kit). Then we'll measure the concentration of the plasmid DNA extracted using a nanodrop spectrophotometer.
Mini-Prep Protocol
- Spin down culture
- Resuspend
Part 2: Restriction Enzyme Digest
We will take the plasmid we just extracted and set up a restriction digest to cut the part out of the plasmid.