Stanford/BIOE44:Module 1:Day2: Difference between revisions
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==Introduction== | ==Introduction== | ||
Last time we digested the color generator gene cassettes out of a plasmid. Now we need to separate the cassette fragment from the backbone fragment. To do this we will perform a gel extraction which has two steps. First we will run our digest reactions out on an agarose gel. This will separate the DNA fragments based on size. We'll cut out the band that corresponds the color generator genes. Then we'll use a kit to purify the DNA out of the the agarose piece. | |||
After we purify the color generator gene cassette, we will insert it into a new vector that has an inducible promoter. | |||
===Background on Methods=== | ===Background on Methods=== | ||
Revision as of 01:30, 4 April 2010
M1: Day 2 - Gel Extraction and Ligation
Introduction
Last time we digested the color generator gene cassettes out of a plasmid. Now we need to separate the cassette fragment from the backbone fragment. To do this we will perform a gel extraction which has two steps. First we will run our digest reactions out on an agarose gel. This will separate the DNA fragments based on size. We'll cut out the band that corresponds the color generator genes. Then we'll use a kit to purify the DNA out of the the agarose piece.
After we purify the color generator gene cassette, we will insert it into a new vector that has an inducible promoter.
