Stanford/BIOE44:Module 1:Day4

Module 1: Day 4

Before Class

Results

Troubleshooting The Results

Introduction to Arsenic Detoxification Genes in Bacteria

Building a New BioBrick Part

The part you are going to design is arsR gene and upstream regulation sequence. Recall that the part includes the nucleotide sequence for the arsR gene that encodes the ArsR protein. The ArsR protein is a repressor protein that binds to the operator sequence upstream of the arsR gene. In this way arsR is autoregulated. That is, the expression of arsR turns off the transcription of more arsR mRNA under normal conditions. However, when arsenic is present, the metal binds to the ArsR protein and prevents it from binding to the operator allowing transcription of both the arsR gene and the genes immediately downstream.

The part you are going to rebuild is Part: BBa_J33201 designed and added to the registry by the Edinburgh 2006 iGem team. You can view the part at [1]

[IMAGE:Part_BBa_JJ33201.JPG]

You could order the part from the registry, but for this class you need to make it from scratch. Indeed the Edinburgh team had could not rely on a catalog. How might you go about this? You should propose two distinct ways to recreate this part.

1. Where might you find this genetic material?
2. How would you be able to get at it?
3. How could you convert a gene to a easy to use biobrick part?

Brainstorm with your classmates and if you need help ask one of the TAs for some hints. Once you have proposed a way to build the part, come find a TA to get a sheet with some further guidelines.

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