Stanford/BIOE44:Module 1:Day6: Difference between revisions
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#Search the [http://partsregistry.org parts registry]. | #Search the [http://partsregistry.org parts registry]. | ||
#When you find a part you want, click on the "get this part" link located on the right. | #When you find a part you want, click on the "get this part" link located on the right. | ||
#If the part is in the 2009 | #If the part is in the 2009 or 2007 distribution, we should have it (but there is a small chance we won't if it has already been used). | ||
#Send an email to Isis with the following information. ''All of this is listed on the 'Get Part' page!'': | #Send an email to Isis with the following information. ''All of this info is listed on the 'Get Part' page!'': | ||
#*Part number | #*Part number | ||
#*Distribution (ex. Spring | #*Distribution (ex. Spring 2007) | ||
#*Plate number | #*Plate number | ||
#*Well number | #*Well number | ||
#*Antibiotic resistance | #*Antibiotic resistance | ||
#Isis will send you a confirmation email containing which parts are available to you. | #Isis will send you a confirmation email containing which parts are available to you. | ||
#Isis will transform the parts into E. coli. You will receive a plate with colonies on it. | #Isis will transform the parts into E. coli. You will receive a plate with colonies on it. This means it will take at least a day to get your parts. | ||
==Some interesting results for those doing a colony PCR== | |||
[[Image:AnotatedAdministrator 2010-04-20 00hr 39min.jpg]] | |||
1% and 0.8% agarose (90V) | |||
'''What can you gather from this gel?''' | |||
Summary of Procedures | |||
# Colony PCR, arsR biobrick primers, MG1655 | |||
# PCR Cleanup Qiagen | |||
# Digestion with EcoRi and SpeI | |||
#Method A: PCR Cleanup Kit only | |||
#Method B: PCR Cleanup Kit + Gel Extraction |
Latest revision as of 11:01, 20 April 2010
M1: Day 6 - Arsenic!
How to find and get parts
- Search the parts registry.
- When you find a part you want, click on the "get this part" link located on the right.
- If the part is in the 2009 or 2007 distribution, we should have it (but there is a small chance we won't if it has already been used).
- Send an email to Isis with the following information. All of this info is listed on the 'Get Part' page!:
- Part number
- Distribution (ex. Spring 2007)
- Plate number
- Well number
- Antibiotic resistance
- Isis will send you a confirmation email containing which parts are available to you.
- Isis will transform the parts into E. coli. You will receive a plate with colonies on it. This means it will take at least a day to get your parts.
Some interesting results for those doing a colony PCR
1% and 0.8% agarose (90V)
What can you gather from this gel?
Summary of Procedures
- Colony PCR, arsR biobrick primers, MG1655
- PCR Cleanup Qiagen
- Digestion with EcoRi and SpeI
- Method A: PCR Cleanup Kit only
- Method B: PCR Cleanup Kit + Gel Extraction