Stanford/BIOE44:Module 2

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(Inventory for Arsenic Sensor)
(Inventory for Arsenic Sensor)
Line 66: Line 66:
| 273 ng/uL
| 273 ng/uL
|}
|}
 +
 +
Sunny, Alex
 +
| pSB2K3 vector (with K274111 insert)
 +
| undigested
 +
| 48 uL
 +
| 8.8 ng/ul
 +
|-
 +
|
 +
| color 002 purple insert
 +
| digested X,P, gel extracted
 +
|
 +
| 0 ng/uL (no DNA retrieved from gels)
 +
|-
 +
|
 +
| arsR insert
 +
| digested E,S
 +
|
 +
| 11.1 ng/uL
 +
|-
 +
|
 +
| S03511 vector (low-medium copy #, inducible)
 +
| undigested
 +
| 48uL
 +
| 41.2 ng/uL
 +
|-
 +
|
 +
| I3023 vector (low-medium copy #, inducible)
 +
| undigested
 +
| 48uL
 +
| 97.2 ng/uL
 +
|-
 +
|
 +
| I3023 vector (low-medium copy #, inducible)
 +
| undigested
 +
| 48uL
 +
| 93.7 ng/uL
 +
|-
 +
| I3002 vector (low-medium copy #, constitutive)
 +
| undigested
 +
| 48uL
 +
| 103.9 ng/uL-
 +
|
 +
| -
 +
| I3002 vector (low-medium copy #, constitutive)
 +
| undigested
 +
| 48uL
 +
| 109.1 ng/uL-
 +
|
==Introduction==
==Introduction==

Revision as of 19:06, 22 April 2010

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Contents

Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members Part Status Volume Concentration
Daniel and Aaditya arsR insert Digested at E and S 25 uL 36.8 ng/uL
arsR insert Digested at X and S, extracted from gel 2 gel bands (hasn't been purified yet)
Travis and Scott none
Frank, Claire, and Sanjay pSB2k3 vector (with K274111 insert) undigested a substantial amount 203 ng/ul
color 004 light green insert digested X,P and running in gels ~15uL 110 ng/uL
arsR insert digested E,S and running in gels ~15 uL 133 ng/uL
color 004 light green insert digested X,P and not running in gels yet 40 uL 110 ng/uL
arsR digested E,S and not running in gels yet 40 uL 133 ng/uL
MG1655 vector (low copy vector and red-orange insert) digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet 25uL each 273 ng/uL

Sunny, Alex | pSB2K3 vector (with K274111 insert) | undigested | 48 uL | 8.8 ng/ul |- | | color 002 purple insert | digested X,P, gel extracted | | 0 ng/uL (no DNA retrieved from gels) |- | | arsR insert | digested E,S | | 11.1 ng/uL |- | | S03511 vector (low-medium copy #, inducible) | undigested | 48uL | 41.2 ng/uL |- | | I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 97.2 ng/uL |- | | I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 93.7 ng/uL |- | I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 103.9 ng/uL- | | - | I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 109.1 ng/uL- |

Introduction

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Schedule

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