Stanford/BIOE44:Module 2

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(Inventory for Arsenic Sensor)
(Inventory for Arsenic Sensor)
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| 25uL each
| 25uL each
| 273 ng/uL
| 273 ng/uL
-
|}
 
Sunny, Alex
Sunny, Alex
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| 93.7 ng/uL
| 93.7 ng/uL
|-
|-
 +
|
| I3002 vector (low-medium copy #, constitutive)
| I3002 vector (low-medium copy #, constitutive)
| undigested
| undigested
| 48uL
| 48uL
-
| 103.9 ng/uL-
+
| 103.9 ng/uL
 +
|-
|
|
-
| -
 
| I3002 vector (low-medium copy #, constitutive)
| I3002 vector (low-medium copy #, constitutive)
| undigested
| undigested
| 48uL
| 48uL
-
| 109.1 ng/uL-
+
| 109.1 ng/uL
-
|
+
|-
 +
|}
==Introduction==
==Introduction==

Revision as of 19:08, 22 April 2010

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DNA Engineering        Devices        Synthesis        Baking        Testing

Contents

Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members Part Status Volume Concentration
Daniel and Aaditya arsR insert Digested at E and S 25 uL 36.8 ng/uL
arsR insert Digested at X and S, extracted from gel 2 gel bands (hasn't been purified yet)
Travis and Scott none
Frank, Claire, and Sanjay pSB2k3 vector (with K274111 insert) undigested a substantial amount 203 ng/ul
color 004 light green insert digested X,P and running in gels ~15uL 110 ng/uL
arsR insert digested E,S and running in gels ~15 uL 133 ng/uL
color 004 light green insert digested X,P and not running in gels yet 40 uL 110 ng/uL
arsR digested E,S and not running in gels yet 40 uL 133 ng/uL
MG1655 vector (low copy vector and red-orange insert) digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet 25uL each 273 ng/uL

Sunny, Alex

pSB2K3 vector (with K274111 insert) undigested 48 uL 8.8 ng/ul
color 002 purple insert digested X,P, gel extracted 0 ng/uL (no DNA retrieved from gels)
arsR insert digested E,S 11.1 ng/uL
S03511 vector (low-medium copy #, inducible) undigested 48uL 41.2 ng/uL
I3023 vector (low-medium copy #, inducible) undigested 48uL 97.2 ng/uL
I3023 vector (low-medium copy #, inducible) undigested 48uL 93.7 ng/uL
I3002 vector (low-medium copy #, constitutive) undigested 48uL 103.9 ng/uL
I3002 vector (low-medium copy #, constitutive) undigested 48uL 109.1 ng/uL

Introduction

TBA

Schedule

TBA
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