Stanford/BIOE44:Module 2: Difference between revisions
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|Kosh & Isis | |Kosh & Isis | ||
| Purple Color generator | | Purple Color generator | ||
| cut at XbaI & PstI, gel extracted | | cut at XbaI & PstI, gel extracted (ready for ligation) | ||
| ~5uL | | ~5uL | ||
| | | | ||
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| | | | ||
|Dark Green Color generator | |Dark Green Color generator | ||
|cut at XbaI & PstI, gel extracted | |cut at XbaI & PstI, gel extracted (ready for ligation) | ||
|~5uL | |~5uL | ||
| | | | ||
Line 134: | Line 134: | ||
| | | | ||
|Light Green Color generator | |Light Green Color generator | ||
|cut at XbaI & PstI, gel extracted | |cut at XbaI & PstI, gel extracted (ready for ligation) | ||
| ~5uL | | ~5uL | ||
| | | |
Revision as of 18:34, 24 April 2010
Module 2: Genetic Devices
Inventory for Arsenic Sensor
Group Members | Part | Status | Volume | Concentration |
Chris and Karina | pSB2k3 (digested (E and P), extracted, purified) | Ready for Ligation | 25 uL | 80 ng/uL |
Daniel and Aaditya | arsR insert | Digested at E and S | 25 uL | 36.8 ng/uL |
arsR insert | Digested at X and S, extracted from gel | 2 gel bands | (hasn't been purified yet) | |
Travis and Scott | none | |||
Frank, Claire, and Sanjay | pSB2k3 vector (with K274111 insert) | undigested | a substantial amount | 203 ng/ul |
color 004 light green insert | digested X,P and running in gels | ~15uL | 110 ng/uL | |
arsR insert | digested E,S and running in gels | ~15 uL | 133 ng/uL | |
color 004 light green insert | digested X,P and not running in gels yet | 40 uL | 110 ng/uL | |
arsR | digested E,S and not running in gels yet | 40 uL | 133 ng/uL | |
MG1655 vector (low copy vector and red-orange insert) | digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | 25uL each | 273 ng/uL | |
Sunny, Alex | pSB2K3 vector (with K274111 insert) | undigested | 48 uL | 8.8 ng/ul |
color 002 purple insert | digested X,P, gel extracted | 0 ng/uL (no DNA retrieved from gels) | ||
arsR insert | digested E,S | 11.1 ng/uL | ||
S03511 vector (low-medium copy #, inducible) | undigested | 48uL | 41.2 ng/uL | |
I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 97.2 ng/uL | |
I3023 vector (low-medium copy #, inducible) | undigested | 48uL | 93.7 ng/uL | |
I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 103.9 ng/uL | |
I3002 vector (low-medium copy #, constitutive) | undigested | 48uL | 109.1 ng/uL | |
Kosh & Isis | Purple Color generator | cut at XbaI & PstI, gel extracted (ready for ligation) | ~5uL | |
Dark Green Color generator | cut at XbaI & PstI, gel extracted (ready for ligation) | ~5uL | ||
Light Green Color generator | cut at XbaI & PstI, gel extracted (ready for ligation) | ~5uL |
Introduction
TBA
Schedule
TBA