Stanford/BIOE44:Module 2

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Revision as of 18:13, 27 April 2010

Home        People        Schedule        Key Info.        OWW Basics       
DNA Engineering        Devices        Synthesis        Baking        Testing

Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members Part Status Volume Concentration
Chris and Karina pSB2k3 (digested (E and P), extracted, purified) Ready for Ligation 25 uL 80 ng/uL
Daniel and Aaditya arsR insert Digested at E and S 25 uL 36.8 ng/uL
arsR insert Digested at X and S, extracted from gel 2 gel bands (hasn't been purified yet)
Travis and Scott none
Frank, Claire, and Sanjay pSB2k3 vector (with K274111 insert) undigested a substantial amount 203 ng/ul
color 004 light green insert digested X,P and running in gels ~15uL 110 ng/uL
arsR insert digested E,S and running in gels ~15 uL 133 ng/uL
color 004 light green insert digested X,P and not running in gels yet 40 uL 110 ng/uL
arsR digested E,S and not running in gels yet 40 uL 133 ng/uL
MG1655 vector (low copy vector and red-orange insert) digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet 25uL each 273 ng/uL
Sunny, Alex color 002 purple insert digested X,P, gel extracted 0 ng/uL (no DNA retrieved from gels)
arsR insert digested E,S, gel extracted 0 ng/uL (no DNA retrieved from gels)
pSB2K3 vector (with K274111 insert) undigested 48 uL 8.8 ng/ul
arsR insert digested E,S ??? 11.1 ng/uL
S03511 vector (low-medium copy #, inducible) undigested 48uL 41.2 ng/uL
J13023 vector (low-medium copy #, inducible) undigested 48uL 97.2 ng/uL
J13023 vector (low-medium copy #, inducible) undigested 48uL 93.7 ng/uL
J13002 vector (low-medium copy #, constitutive) undigested 48uL 103.9 ng/uL
J13002 vector (low-medium copy #, constitutive) undigested 48uL 109.1 ng/uL
Kosh & Isis Purple Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
Dark Green Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
Light Green Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
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