Stanford/BIOE44:Module 2

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(Module 2: Moss)
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(Inventory for Arsenic Sensor)
 
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=Module 2: Genetic Device Design & Engineering=
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=Module 2: Genetic Devices=
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==Inventory for Arsenic Sensor==
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{| style="width:95%" border=1px
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|'''Group Members'''
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|'''Part'''
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|'''Status'''
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|'''Volume'''
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|'''Concentration'''
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|-
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| Chris and Karina
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| pSB2k3 (digested (E and P), extracted, purified)
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| Ready for Ligation
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| 25 uL
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| 80 ng/uL
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|-
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| Daniel and Aaditya
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| arsR insert
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| Digested at E and S
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| 25 uL
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| 36.8 ng/uL
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|-
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|
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| arsR insert
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| Digested at X and S, extracted from gel
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| 2 gel bands
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| (hasn't been purified yet)
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|-
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| Travis and Scott
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| none
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|
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|
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|
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|-
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| Frank, Claire, and Sanjay
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| pSB2k3 vector (with K274111 insert)
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| undigested
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| a substantial amount
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| 203 ng/ul
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|-
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|
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| pSB2K3 vector (low copy vector and red-orange insert)
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| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet
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| 25uL each
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| 273 ng/uL
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|-
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|
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| color 004 light green insert
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| digested X,P and running in gels
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| ~15uL
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| 110 ng/uL
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|-
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|
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| arsR insert
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| digested E,S and running in gels
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| ~15 uL
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| 133 ng/uL
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|-
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|
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| color 004 light green insert
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| digested X,P and not running in gels yet
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| 40 uL
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| 110 ng/uL
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|-
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|
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| arsR
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| digested E,S and not running in gels yet
 +
| 40 uL
 +
| 133 ng/uL
 +
|-
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|Sunny, Alex
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| color 002 purple insert
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| digested X,P, gel extracted
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|
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| 0 ng/uL (no DNA retrieved from gels)
 +
|-
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|
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|arsR insert
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|digested E,S, gel extracted
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|
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|0 ng/uL (no DNA retrieved from gels)
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|-
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|
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| pSB2K3 vector (with K274111 insert)
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| undigested
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| 48 uL
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| 8.8 ng/ul
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|-
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|
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| S03511 vector (low-medium copy #, inducible)
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| undigested
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| 0uL
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| 41.2 ng/uL
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|-
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|
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| J13023-1 vector (low-medium copy #, inducible)
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| undigested
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| 27uL
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| 97.2 ng/uL
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|-
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|
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| J13023-2 vector (low-medium copy #, inducible)
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| undigested
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| 27uL
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| 93.7 ng/uL
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|-
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|
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| J13002-1 vector (low-medium copy #, constitutive)
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| undigested
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| 28uL
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| 103.9 ng/uL
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|-
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|
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| J13002-2 vector (low-medium copy #, constitutive)
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| undigested
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| 29uL
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| 109.1 ng/uL
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|-
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|Kosh & Isis
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| Purple Color generator
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| cut at XbaI & PstI, gel extracted (ready for ligation)
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| ~5uL
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|
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|-
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|
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|Dark Green Color generator
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|cut at XbaI & PstI, gel extracted (ready for ligation)
 +
|~5uL
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|
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|-
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|
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|Light Green Color generator
 +
|cut at XbaI & PstI, gel extracted (ready for ligation)
 +
| ~5uL
 +
|
 +
|-
 +
|}

Current revision

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Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members Part Status Volume Concentration
Chris and Karina pSB2k3 (digested (E and P), extracted, purified) Ready for Ligation 25 uL 80 ng/uL
Daniel and Aaditya arsR insert Digested at E and S 25 uL 36.8 ng/uL
arsR insert Digested at X and S, extracted from gel 2 gel bands (hasn't been purified yet)
Travis and Scott none
Frank, Claire, and Sanjay pSB2k3 vector (with K274111 insert) undigested a substantial amount 203 ng/ul
pSB2K3 vector (low copy vector and red-orange insert) digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet 25uL each 273 ng/uL
color 004 light green insert digested X,P and running in gels ~15uL 110 ng/uL
arsR insert digested E,S and running in gels ~15 uL 133 ng/uL
color 004 light green insert digested X,P and not running in gels yet 40 uL 110 ng/uL
arsR digested E,S and not running in gels yet 40 uL 133 ng/uL
Sunny, Alex color 002 purple insert digested X,P, gel extracted 0 ng/uL (no DNA retrieved from gels)
arsR insert digested E,S, gel extracted 0 ng/uL (no DNA retrieved from gels)
pSB2K3 vector (with K274111 insert) undigested 48 uL 8.8 ng/ul
S03511 vector (low-medium copy #, inducible) undigested 0uL 41.2 ng/uL
J13023-1 vector (low-medium copy #, inducible) undigested 27uL 97.2 ng/uL
J13023-2 vector (low-medium copy #, inducible) undigested 27uL 93.7 ng/uL
J13002-1 vector (low-medium copy #, constitutive) undigested 28uL 103.9 ng/uL
J13002-2 vector (low-medium copy #, constitutive) undigested 29uL 109.1 ng/uL
Kosh & Isis Purple Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
Dark Green Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
Light Green Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
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