Stanford/BIOE44:Module 2

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(Inventory for Arsenic Sensor)
Current revision (16:33, 6 May 2010) (view source)
(Inventory for Arsenic Sensor)
 
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|'''Volume'''
|'''Volume'''
|'''Concentration'''
|'''Concentration'''
 +
|-
 +
| Chris and Karina
 +
| pSB2k3 (digested (E and P), extracted, purified)
 +
| Ready for Ligation
 +
| 25 uL
 +
| 80 ng/uL
|-
|-
| Daniel and Aaditya
| Daniel and Aaditya
Line 35: Line 41:
| a substantial amount
| a substantial amount
| 203 ng/ul
| 203 ng/ul
 +
|-
 +
|
 +
| pSB2K3 vector (low copy vector and red-orange insert)
 +
| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet
 +
| 25uL each
 +
| 273 ng/uL
|-
|-
|  
|  
Line 60: Line 72:
| 133 ng/uL
| 133 ng/uL
|-
|-
 +
|Sunny, Alex
 +
| color 002 purple insert
 +
| digested X,P, gel extracted
 +
|
 +
| 0 ng/uL (no DNA retrieved from gels)
 +
|-
 +
|
 +
|arsR insert
 +
|digested E,S, gel extracted
|
|
-
| MG1655 vector (low copy vector and red-orange insert)
+
|0 ng/uL (no DNA retrieved from gels)
-
| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet
+
-
| 25uL each
+
-
| 273 ng/uL
+
|-
|-
-
|Sunny, Alex
+
|
| pSB2K3 vector (with K274111 insert)
| pSB2K3 vector (with K274111 insert)
| undigested
| undigested
| 48 uL
| 48 uL
| 8.8 ng/ul
| 8.8 ng/ul
-
|-
 
-
|
 
-
| color 002 purple insert
 
-
| digested X,P, gel extracted
 
-
|
 
-
| 0 ng/uL (no DNA retrieved from gels)
 
-
|-
 
-
|
 
-
| arsR insert
 
-
| digested E,S
 
-
|
 
-
| 11.1 ng/uL
 
|-
|-
|
|
| S03511 vector (low-medium copy #, inducible)
| S03511 vector (low-medium copy #, inducible)
| undigested
| undigested
-
| 48uL
+
| 0uL
| 41.2 ng/uL
| 41.2 ng/uL
|-
|-
|
|
-
| I3023 vector (low-medium copy #, inducible)
+
| J13023-1 vector (low-medium copy #, inducible)
| undigested
| undigested
-
| 48uL
+
| 27uL
| 97.2 ng/uL
| 97.2 ng/uL
|-
|-
|
|
-
| I3023 vector (low-medium copy #, inducible)
+
| J13023-2 vector (low-medium copy #, inducible)
| undigested
| undigested
-
| 48uL
+
| 27uL
| 93.7 ng/uL
| 93.7 ng/uL
|-
|-
|
|
-
| I3002 vector (low-medium copy #, constitutive)
+
| J13002-1 vector (low-medium copy #, constitutive)
| undigested
| undigested
-
| 48uL
+
| 28uL
| 103.9 ng/uL
| 103.9 ng/uL
|-
|-
|
|
-
| I3002 vector (low-medium copy #, constitutive)
+
| J13002-2 vector (low-medium copy #, constitutive)
| undigested
| undigested
-
| 48uL
+
| 29uL
| 109.1 ng/uL
| 109.1 ng/uL
|-
|-
-
|}
+
|Kosh & Isis
-
 
+
| Purple Color generator
-
{Sunny, Alex
+
| cut at XbaI & PstI, gel extracted (ready for ligation)
-
| pSB2K3 vector (with K274111 insert)
+
| ~5uL
-
| undigested
+
-
| 48 uL
+
-
| 8.8 ng/ul
+
-
|-
+
|  
|  
-
| color 002 purple insert
 
-
| digested X,P, gel extracted
 
-
|
 
-
| 0 ng/uL (no DNA retrieved from gels)
 
-
|-
 
-
|
 
-
| arsR insert
 
-
| digested E,S
 
-
|
 
-
| 11.1 ng/uL
 
|-
|-
|
|
-
| S03511 vector (low-medium copy #, inducible)
+
|Dark Green Color generator
-
| undigested
+
|cut at XbaI & PstI, gel extracted (ready for ligation)
-
| 48uL
+
|~5uL
-
| 41.2 ng/uL
+
-
|-
+
|
|
-
| I3023 vector (low-medium copy #, inducible)
 
-
| undigested
 
-
| 48uL
 
-
| 97.2 ng/uL
 
|-
|-
|
|
-
| I3023 vector (low-medium copy #, inducible)
+
|Light Green Color generator
-
| undigested
+
|cut at XbaI & PstI, gel extracted (ready for ligation)
-
| 48uL
+
| ~5uL
-
| 93.7 ng/uL
+
|
|-
|-
-
| I3002 vector (low-medium copy #, constitutive)
 
-
| undigested
 
-
| 48uL
 
-
| 103.9 ng/uL-
 
-
|
 
-
| -
 
-
| I3002 vector (low-medium copy #, constitutive)
 
-
| undigested
 
-
| 48uL
 
-
| 109.1 ng/uL-
 
|}
|}
-
 
-
==Introduction==
 
-
TBA
 
-
 
-
==Schedule==
 
-
 
-
TBA
 

Current revision

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Module 2: Genetic Devices

Inventory for Arsenic Sensor

Group Members Part Status Volume Concentration
Chris and Karina pSB2k3 (digested (E and P), extracted, purified) Ready for Ligation 25 uL 80 ng/uL
Daniel and Aaditya arsR insert Digested at E and S 25 uL 36.8 ng/uL
arsR insert Digested at X and S, extracted from gel 2 gel bands (hasn't been purified yet)
Travis and Scott none
Frank, Claire, and Sanjay pSB2k3 vector (with K274111 insert) undigested a substantial amount 203 ng/ul
pSB2K3 vector (low copy vector and red-orange insert) digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet 25uL each 273 ng/uL
color 004 light green insert digested X,P and running in gels ~15uL 110 ng/uL
arsR insert digested E,S and running in gels ~15 uL 133 ng/uL
color 004 light green insert digested X,P and not running in gels yet 40 uL 110 ng/uL
arsR digested E,S and not running in gels yet 40 uL 133 ng/uL
Sunny, Alex color 002 purple insert digested X,P, gel extracted 0 ng/uL (no DNA retrieved from gels)
arsR insert digested E,S, gel extracted 0 ng/uL (no DNA retrieved from gels)
pSB2K3 vector (with K274111 insert) undigested 48 uL 8.8 ng/ul
S03511 vector (low-medium copy #, inducible) undigested 0uL 41.2 ng/uL
J13023-1 vector (low-medium copy #, inducible) undigested 27uL 97.2 ng/uL
J13023-2 vector (low-medium copy #, inducible) undigested 27uL 93.7 ng/uL
J13002-1 vector (low-medium copy #, constitutive) undigested 28uL 103.9 ng/uL
J13002-2 vector (low-medium copy #, constitutive) undigested 29uL 109.1 ng/uL
Kosh & Isis Purple Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
Dark Green Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
Light Green Color generator cut at XbaI & PstI, gel extracted (ready for ligation) ~5uL
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