Stanford/BIOE44:Module 2: Difference between revisions
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| Line 41: | Line 41: | ||
| a substantial amount | | a substantial amount | ||
| 203 ng/ul | | 203 ng/ul | ||
|- | |||
| | |||
| pSB2K3 vector (low copy vector and red-orange insert) | |||
| digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | |||
| 25uL each | |||
| 273 ng/uL | |||
|- | |- | ||
| | | | ||
| Line 65: | Line 71: | ||
| 40 uL | | 40 uL | ||
| 133 ng/uL | | 133 ng/uL | ||
|- | |- | ||
|Sunny, Alex | |Sunny, Alex | ||
| Line 89: | Line 89: | ||
| 48 uL | | 48 uL | ||
| 8.8 ng/ul | | 8.8 ng/ul | ||
|- | |- | ||
| | | | ||
| S03511 vector (low-medium copy #, inducible) | | S03511 vector (low-medium copy #, inducible) | ||
| undigested | | undigested | ||
| | | 0uL | ||
| 41.2 ng/uL | | 41.2 ng/uL | ||
|- | |- | ||
| | | | ||
| J13023 vector (low-medium copy #, inducible) | | J13023-1 vector (low-medium copy #, inducible) | ||
| undigested | | undigested | ||
| | | 27uL | ||
| 97.2 ng/uL | | 97.2 ng/uL | ||
|- | |- | ||
| | | | ||
| J13023 vector (low-medium copy #, inducible) | | J13023-2 vector (low-medium copy #, inducible) | ||
| undigested | | undigested | ||
| | | 27uL | ||
| 93.7 ng/uL | | 93.7 ng/uL | ||
|- | |- | ||
| | | | ||
| J13002 vector (low-medium copy #, constitutive) | | J13002-1 vector (low-medium copy #, constitutive) | ||
| undigested | | undigested | ||
| | | 28uL | ||
| 103.9 ng/uL | | 103.9 ng/uL | ||
|- | |- | ||
| | | | ||
| J13002 vector (low-medium copy #, constitutive) | | J13002-2 vector (low-medium copy #, constitutive) | ||
| undigested | | undigested | ||
| | | 29uL | ||
| 109.1 ng/uL | | 109.1 ng/uL | ||
|- | |- | ||
Latest revision as of 13:33, 6 May 2010
Module 2: Genetic Devices
Inventory for Arsenic Sensor
| Group Members | Part | Status | Volume | Concentration |
| Chris and Karina | pSB2k3 (digested (E and P), extracted, purified) | Ready for Ligation | 25 uL | 80 ng/uL |
| Daniel and Aaditya | arsR insert | Digested at E and S | 25 uL | 36.8 ng/uL |
| arsR insert | Digested at X and S, extracted from gel | 2 gel bands | (hasn't been purified yet) | |
| Travis and Scott | none | |||
| Frank, Claire, and Sanjay | pSB2k3 vector (with K274111 insert) | undigested | a substantial amount | 203 ng/ul |
| pSB2K3 vector (low copy vector and red-orange insert) | digested with E,P (for double ligation) and X,P (for single ligation); not running in gels yet | 25uL each | 273 ng/uL | |
| color 004 light green insert | digested X,P and running in gels | ~15uL | 110 ng/uL | |
| arsR insert | digested E,S and running in gels | ~15 uL | 133 ng/uL | |
| color 004 light green insert | digested X,P and not running in gels yet | 40 uL | 110 ng/uL | |
| arsR | digested E,S and not running in gels yet | 40 uL | 133 ng/uL | |
| Sunny, Alex | color 002 purple insert | digested X,P, gel extracted | 0 ng/uL (no DNA retrieved from gels) | |
| arsR insert | digested E,S, gel extracted | 0 ng/uL (no DNA retrieved from gels) | ||
| pSB2K3 vector (with K274111 insert) | undigested | 48 uL | 8.8 ng/ul | |
| S03511 vector (low-medium copy #, inducible) | undigested | 0uL | 41.2 ng/uL | |
| J13023-1 vector (low-medium copy #, inducible) | undigested | 27uL | 97.2 ng/uL | |
| J13023-2 vector (low-medium copy #, inducible) | undigested | 27uL | 93.7 ng/uL | |
| J13002-1 vector (low-medium copy #, constitutive) | undigested | 28uL | 103.9 ng/uL | |
| J13002-2 vector (low-medium copy #, constitutive) | undigested | 29uL | 109.1 ng/uL | |
| Kosh & Isis | Purple Color generator | cut at XbaI & PstI, gel extracted (ready for ligation) | ~5uL | |
| Dark Green Color generator | cut at XbaI & PstI, gel extracted (ready for ligation) | ~5uL | ||
| Light Green Color generator | cut at XbaI & PstI, gel extracted (ready for ligation) | ~5uL |
